Course Overview
The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions.
Program
This 5-days course will cover the following topics:
Overview of technologies for single cells omics and fundamentals of NGS (0.5 day); Separation of single cells by FACS (0.5 day); Single cell RNA prep by droplet-based assays (10xGenomics) (0.5 day); Preparation and quantification of single cell RNA-Seq libraries (1 day); Introduction to microfluidics-based approaches for single cell profiling (0.5 day); Basics of single cell transcriptome data analysis using state-of-the-art data analysis pipelines (2 days).
Quality Controls, best practices and “tips and tricks” will be discussed.
Audience
Scientists, students and Core Facility staff interested in applying advanced single-cell transcriptome sequencing in their research are welcome. Basic knowledge of molecular biology and basics in “R” are desirable
Online registration: webcampus.pasteur.fr/ip/single-cel
Co-directors:
Valentina LIBRI
Milena HASAN
Deadline for application: 30th April 2022
Attendees: 12 participants
Contact: formation@pasteur.fr
Localisation
Localisation: 28 Rue du Docteur Roux, Paris, France
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* This function will render a Google Map onto the selected jQuery element
*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
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* This function will add a marker to the selected Google Map
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* @type function
* @date 8/11/2013
* @since 4.3.0
*
* @param $marker (jQuery element)
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function add_marker( $marker, map ) {
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* @date 8/11/2013
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*
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