Animation / Soutien

Le SPAIS vient en soutien aux référents pour les aider à promouvoir leur Action Technologique Ciblée “Single Cell Resources Initiative” au travers de la mise en place de différentes actions:

  • Animations
  • Ressourcements
  • Formations

Personnes contact :

Mallory Perrin-Wolff & Sylvie Gratepanche

New technologies

New droplet-microfluidic technologies at CB UTechS

CB UTechS is glad to announce the incorporation of two droplet-based microfluidic systems into our technological portfolio, available within the P2+ environment in collaboration with the labs of J.Enninga, C.Baroud, ESPCI and the start-up Okomera.

Microfluidics is a powerful tool that integrates miniaturization (decreased reagent use), automation (decreased bias due to manipulation) and high throughput. Droplet-based microfluidics has become one of the most widely used microfluidics techniques. It is highly versatile and can be used in conjunction with single-cell omics, cell-cell interaction studies, drug screenings and it is adaptable for analysis of the different biological samples used by the Pasteur community – bacteria, fungi and eukaryotic cells.  The two novel technologies are:

  • Multiscreen for establishment and studies of 3D-cultures in 50nL or 450nL droplets. Seed up to 1200 spheroids in parallel, and control timely addition of liquid droplets, containing cells, drugs or gels. Quantify growth, viability, and other bright-field and fluorescent biomarkers thanks to automated epifluorescence microscopy and Okomera’s image analysis software.

  • MicDrop for fluorescence-based single cell / single microorganism analysis and sorting of droplet-enclosed samples for downstream RNA-Seq, DNA-Seq or single-cell secretion assays. Currently available for analysis and sorting of P2-level microorganisms, it will be open for experimentation on P2+ pathogens starting from autumn 2021.