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© Service photographie
Vue sur les capillaires d'un séquenceur d'ADN de la Plate Forme Génomique.
Scientific Fields
Diseases
Organisms
Applications
Technique

About

The Center for Human Immunology provides access to cutting-edge technologies that allow single cell profiling & sequencing as well as imaging flow cytometry and live cell imaging. Most of the technologies are located within a BSL2+ confined space to allow working with human samples and pathogens.

Access protocol: Connect to the electronic project submission  and the PPMS account to obtain access to the CRT technologies. Contact us at CRT-tc@pasteur.fr

labo

Software

Services

Open desk CRT

The Center for Translational Science (CRT) offers assistance for scientists in the performance of their research by providing: support for the preparation of clinical operations, access to human bioresources (including from healthy volunteers) cutting-edge […]

Training

The team provides access to and training for the technologies and data-analysis software available in the Center and ensures proper functioning of the systems. In addition, the research engineers propose scientific collaborations using these […]

CRT Technologies – Use Fees

Cost for the use of technologies

Equipments

Cell metabolism • Seahorse • XFp Analyzer - BSL2/P2+

Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- or […]

Live cell imaging • Essen Bioscience • Incucyte - BSL2/P2+

Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]

Medium-throughput Protein quantification • Luminex • Magpix

Bead-based multiplex ELISA technology that utilizes LED technology and digital photography for simultaneous quantification of up to 50 analytes in a single microplate well. 

Next generation sequencing • Life technologies • Ion Proton & Personal Genome Machine

Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity.The technologies are completed with the Ion Chef System to enable automated, high-throughput template preparation and reproducible chip loading. […]

Gene expression profiling • nCounter Dx Analysis System • NanoString Technologies

A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows hundreds of mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and single molecule imaging, with […]

High-throughput gene expression and genotyping • Illumina • Iscan

A high-precision scanner for SNP genotyping, CNV analysis, DNA methylation, and gene expression profiling.  

Single-cell cDNA preparation • Fluidigm • C1 - BSL2/P2+

Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell applications, allowing […]

Medium-throughput gene expression profiling • Fluidigm • BioMark

Microfluidic-based technology  for gene expression analysis (96 genes*96 samples), SNP genotyping, digital PCR, mutant detection and real-time PCR to qualify and quantitate samples prior to next-generation sequencing.

RNA and DNA quality/quantity • Agilent • 2100 Bioanalyzer

Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA quality control measurements before downstream experiments.

DNA/RNA quantification • Life Technologies • Qubit ® 2.0 Fluorometer

Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.

Cell sorter • BD • Aria II (BSL2+)

Equipped with 5 lasers (355nm, 405nm, 488nm, 561nm and 640nm), they allow bulk- or single-cell sorting into 96-well plates with “index sorting”   Easy tool to build your panels: BD FACS ARIA II Charlie Brown […]

Flow cytometry analyzer • BD • LSRII (Bsl2+)

Equipped with four laser lines (355nm, 405nm, 488nm and 640nm), 17 fluorescent detectors and an HTS unit for high-throughput screening in plates

Imaging cytometry, ImageStreamX • Amnis • ISX

Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]

Publications