The Center for Human Immunology provides access to cutting-edge technologies that allow single cell profiling & sequencing as well as imaging flow cytometry and live cell imaging. Most of the technologies are located within a BSL2+ confined space to allow working with human samples and pathogens.
INCEPTION – Institut Convergence for the study of Emergence of Pathology Through Individuals and Populations
Inception Goal The Inception’s goal is to develop a core structure to mobilize data resources, numerical sciences, and fundamental experimental biology in a range of health issues. Inception program uses Integrative Biology, Social Science […]
intuitive web-based applications for quickly analyzing and accurately interpreting the biological meaning in real-time PCR, sequencing or microarray data.
software developed for the analysis of ImageStreamX data; it evaluates over 35 features for each image and over 200 features per cell and integrates population statistics and imagery – every dot on a scatter […]
The Center for Translational Science (CRT) offers assistance for scientists in the performance of their research by providing: support for the preparation of clinical operations, access to human bioresources (including from healthy volunteers) cutting-edge […]
The team provides access to and training for the technologies and data-analysis software available in the Center and ensures proper functioning of the systems. In addition, the research engineers propose scientific collaborations using these […]
Cost for the use of technologies
Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- or […]
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]
Bead-based multiplex ELISA technology that utilizes LED technology and digital photography for simultaneous quantification of up to 50 analytes in a single microplate well.
Nucleofection in 96 well shuttle
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity.The technologies are completed with the Ion Chef System to enable automated, high-throughput template preparation and reproducible chip loading. […]
A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows hundreds of mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and single molecule imaging, with […]
A high-precision scanner for SNP genotyping, CNV analysis, DNA methylation, and gene expression profiling.
Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell applications, allowing […]
Microfluidic-based technology for gene expression analysis (96 genes*96 samples), SNP genotyping, digital PCR, mutant detection and real-time PCR to qualify and quantitate samples prior to next-generation sequencing.
Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA quality control measurements before downstream experiments.
Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
Equipped with 5 lasers (355nm, 405nm, 488nm, 561nm and 640nm), they allow bulk- or single-cell sorting into 96-well plates with “index sorting” Easy tool to build your panels: BD FACS ARIA II Charlie Brown […]
Equipped with four laser lines (355nm, 405nm, 488nm and 640nm), 17 fluorescent detectors and an HTS unit for high-throughput screening in plates
Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
2017Single-cell gene expression using multiplex RT-qPCR to characterize heterogeneity of rare lymphoid populations, J. Vis. Exp. JoVE 2017 Jan 19; (119).
2016Standardized Whole-Blood Transcriptional Profiling Enables the Deconvolution of Complex Induced Immune Responses, DOI: http://dx.doi.org/10.1016/j.celrep.2016.08.011 show.
2016Inhibition of DPP4 activity in humans establishes its in vivo role in CXCL10 post-translational modification: prospective placebo-controlled clinical studies, EMBO Mol Med 2016 Jun;8(6):679-83.
2016Protein biomarkers discriminate Leishmania major-infected and non-infected individuals in areas endemic for cutaneous leishmaniasis, BMC Infect. Dis. 2016;16:138.
2014Baseline sensitivity of T cells to alpha-IFN correlates with sustained virological response to IFN-based triple therapy in HCV infection, J. Viral Hepat. 2015 Jun;22(6):524-34.
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