High-throughput sequencing has revolutionized our abilities to track and characterize novel bacterial strains as they emerge and disseminate globally. Genome-based epidemiological typing systems provide ultimate resolution for strain discrimination. Genome sequences may also be used to extract medically important features such as virulence and resistance genes in real time. In this project, we set-up and maintain genomic libraries and strain nomenclatures for bacterial pathogens of public health importance, including Klebsiella pneumoniae and Listeria monocytogenes. The genomic databases are accessible at https://bigsdb.pasteur.fr.