Expanding the toolbox for genetic manipulation of Leptospira spp.
In comparison to other bacterial species, genetics and molecular basis of the pathogenesis of leptospires are in their infancy. An improved understanding of leptospiral pathogenesis requires reliable genetic tools for functional genetic analysis of […]
Phenotypic characterization of transposon mutants in the pathogen L. interrogans
Site-directed homologous recombination has not been successful in pathogenic Leptospira and there exist only a few examples of mutants obtained by targeted mutagenesis. We have generated a library of random mutants in the pathogen […]
Cell morphology and motility in Leptospira spp.
Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape and the presence of periplasmic flagella. We showed that peptidoglycan sacculi […]
Exoproteins and Leucin-Rich Repeat (LRR) proteins in Leptospira spp.
The roles of leptospiral exoproteins in the mechanisms of disease manifestation in the host have not been determined. We assessed the composition and quantity of exoproteins of pathogenic L. interrogans and their contribution to […]
Development and evaluation of rapid diagnostic tests on leptospirosis
Early laboratory diagnosis of leptospirosis is important to provide appropriate treatment of patients and to take rapid measures in case of an outbreak. Unfortunately, few tests possess both high sensitivity and specificity; this is […]
Molecular and serological investigations on leptospirosis
The genus Leptospira includes 35 Leptospira species, including L. mayottensis (see below), and more than 300 serovars have been described. Little is known about the circulating etiological agents of leptospirosis in most of the […]
2019Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens, Front Microbiol 2019;10:1691.
2019Leptospirosis under the bibliometrics radar: evidence for a vicious circle of neglect, J Glob Health 2019 Jun;9(1):010302.
2019Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics, PLoS Negl Trop Dis 2019 May;13(5):e0007270.
2019Genus-wide Leptospira core genome multilocus sequence typing for strain taxonomy and global surveillance, PLoS Negl Trop Dis 2019 Apr;13(4):e0007374.
2019Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira, Sci Rep 2019 Feb;9(1):1839.
2019A public database for the new MLST scheme for subsp. : surveillance and epidemiology of the causative agent of syphilis, PeerJ 2019;6:e6182.
2018An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins, Cell. Microbiol. 2019 02;21(2):e12949.
2018Isolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis, PLoS Negl Trop Dis 2018 Sep;12(9):e0006694.
2018An extracellular Leptospira interrogans leucine‐rich repeat protein binds human E‐ and VE‐cadherins, Eshghi, A, Gaultney, RA, England, P, et al. An extracellular Leptospira interrogans leucine‐rich repeat protein binds human E‐ and VE‐cadherins. Cellular Microbiology. 2019; 21:e12949. .
2018International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Leptospiraceae. Minutes of the closed meeting, 28 November 2017, Palmerston North, New Zealand, Int. J. Syst. Evol. Microbiol. 2018 Oct;68(10):3362.
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