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  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
09
Feb 2017
Status
Ongoing
Members
1
Structures
1

About

We provide users with expertise in routine and advanced sample preparation and imaging techniques.

Depending on the question we embed samples by routine room temperature embedding and thin sectioning. We provide expertise in preservation and embedding by high-pressure freezing and freeze substitution. More advanced techniques include cryo-EM of vitreous sections. To localize proteins (or certain lipids) we use EM-immuno-labeling of thawed cryo-sections according to Tokuyasu.

Imaging includes routine scanning- and transmission EM, 3D EM by electron tomography or focused ion beam-SEM as well as cryo-EM of particles. We provide assistance in reconstructions and rendering for tomography and FIB-SEM. Together with the imagopole we provide support in developing work-flows for correlated light- and electron microscopy adapted to the questions asked. Finally, we provide help in quantitative analyses of the observations.

Our team will assist you in choosing the appropriate methods for sample preparation, imaging and data analyses.

Access Protocol

New projects should be requested via our online system PPMS. You will be asked to present your question briefly in our team.

All samples with a biosafety level P2/P3 require a ‘protocol de recherche’ to document their procedure of inactivation. Be prepared that the acceptance of this can take some time, depending on the complexity of the samples.

Equipments

Transmission Electron Cryo Microscope • FEI • Tecnai F20

The Tecnai F20 is a 200 kV Transmission Electron Microscope (TEM) specifically designed for high resolution data collection and tomography. The Field Emission Gun (FEG) provides high beam intensity and stability. Further structural analysis […]

Focused Ion Beam Scanning Electron Microscope • Zeiss • Auriga

  Focused ion beam scanning EM is typically applied to analyze whole cells in 3D. In 2016 Atlas-5 was installed on our Auriga with the goal to establish the automated imaging of serial sections, […]

Scanning Electron Microscope • Jeol • 6700F

SEM is typically applied to image the surface of samples. The Jeol 6700F is a routine SEM that can readily be handled by our users after training. It is also equipped with a cryo-transfer […]

CryoCLEM Microscope • Leica • Leica EM Cryo CLEM

Correlative light and electron microscopy (CLEM) can provide localisation and identification solutions of rare events/structures in crowded environments. Electron microscopy grids can be mounted on the Leica EM Cryo CLEM microscope. Full grid fluorescent […]