Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.
Scientific Fields
Diseases
Organisms
Applications
Technique

Optical Biosystems • StellarVision

Target-based, cell-based and phenotypic/microscopy screening assay have numerous advantages and disadvantages and an important challenge in the near future will be to effectively incorporate new screening technologies into phenotypic screening approaches since the traditional limitation of some phenotypic assays is a considerably lower throughput than target-based assays. A significant impediment to performing microscopy/phenotypic-based screens with small living organisms is the inability to develop microscopy-based assay with high resolution imaging over hundreds if not thousands of cells simultaneously. This hurdle has been recently overcome by the acquisition of the “StellarVision” instrument (Optical biosystems Santa Clara USA, ex Light speed genomics, Novato, USA) in Institut Pasteur.

This automated microscope relies on a unique image acquisition system, which based on a synthetic aperture optical system (ODS). Briefly, all conventional microscopes are limited in resolution by their numerical aperture (NA = n.sinΘ). By the SAO system, it is possible by illuminating the sample by powerful DS4 lasers at different angles to collect spatial frequencies, not accessible with a conventional numerical aperture. After processing the signals using an appropriate algorithm, a “sub-pixel” resolution (64 SAO for each physical pixel of the CCD camera) can be achieved, and thus the resolution of the image can be increased for a low objective, while keeping the field of view (FOV). It is possible for example with a 20x low objective without immersion and the SAO technology to obtain an image resolution equivalent to that obtained with a conventional microscope with a 100x oil immersion objective. The implementation of this SAO technology therefore increases the speed of acquisition of high resolution images with a FOV which is 25x higher than with a conventional microscope, allowing a simultaneous analysis of very large number of cells at a high resolution.

For more information, please contact Alix Boucharlat (alix.boucharlat@pasteur.fr)