The research interests of the laboratory for Bioorganic Chemistry of Nucleic acids reside in the synthesis of activated building blocks of chemically modified nucleoside analogs for their use in selection experiments for the crafting of functional nucleic acids with enhanced activities. Particularly, we strive to generate modified aptamers to be used as tools for medical imaging applications and the detection of specific targets such as enzymes, peptides, cancer cells, and small molecules. In addition, we are developing catalytic nucleic acids for the detection of DNA lesions as well as for their use as synthetic tools in molecular biology.
Synthesis of chemically modified nucleoside triphosphates
Functional nucleic acids (aptamers, DNAzymes, ribozymes, and aptazymes) are generated by in vitro Darwinian evolution methods (SELEX and related methods of in vitro selection). We are interested to expand the capacity of these functional […]
Equipped with an ATM-BBO probe and a BACS-60 auto sampler.
Equipped with an ElectroSpray ionisation probe and an Alliance HPLC system.
The H-8 DNA synthesizer is a robust DNA synthesizer that allows for the parallel synthesis of up to 8 DNA, RNA, and modified nucleic acids. In addition, the synthesizer has a low consumption of […]
Our HPLC system is dedicated to the purification of modified oligonucleotides and nucleoside triphosphates. The purifications are usually carried out by anion exchange chromatography (analytical column from GE Healthcare) and reversed-phase HPLC (analytical and […]
Our bioanalyzer is dedicated to the quantification of PCR products stemming from in vitro selection pools for aptamers and DNA enzymes (DNAzymes) using natural and modified nucleic acids. For more information, see www.agilent.com
2017Nucleic Acid Aptamers: Emerging Applications in Medical Imaging, Nanotechnology, Neurosciences, and Drug Delivery, Int. J. Mol. Sci. 2017, 18, 2430.
2017Facile immobilization of DNA using an enzymatic his-tag mimic, Chem. Commun., 2017, 53, 13031-13034.
2017Enzymatic Synthesis of 7’,5’-Bicyclo-DNA Oligonucleotides, Chem. Asian J. 2017, 12, 1347-1352.
2017On the enzymatic incorporation of an imidazole nucleotide into DNA, Org. Biomol. Chem. 2017, 15, 4449-4455.
2017New synthetic route to ethynyl-dUTP: A means to avoid formation of acetyl and chloro vinyl base-modified triphosphates that could poison SELEX experiments, Bioorg. Med. Chem. Lett. 2017, 27, 897-900.
2016Rolling circle amplification with chemically modified nucleoside triphosphates, Curr. Protoc. Nucleic Acid Chem. 2016, 67, 7.26.1-7.26.15.
2015DNA Catalysis: The Chemical Repertoire of DNAzymes, Molecules 2015;20(11):20777-804.
2015Generation of long, fully modified, and serum-resistant oligonucleotides by rolling circle amplification, Org. Biomol. Chem. 2015 Oct;13(38):9820-4.
2015Generation of Aptamers with an Expanded Chemical Repertoire, Molecules 2015;20(9):16643-71.
2015Probing the effect of minor groove interactions on the catalytic efficiency of DNAzymes 8-17 and 10-23, Mol Biosyst 2015 May;11(5):1454-61.
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