The research interests of the laboratory for Bioorganic Chemistry of Nucleic acids reside in the synthesis of activated building blocks of chemically modified nucleoside analogs for their use in selection experiments for the crafting of functional nucleic acids with enhanced activities. Particularly, we strive to generate modified aptamers to be used as tools for medical imaging applications and the detection of specific targets such as enzymes, peptides, cancer cells, and small molecules. In addition, we are developing catalytic nucleic acids for the detection of DNA lesions as well as for their use as synthetic tools in molecular biology.
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Equipments
NMR spectrometer • Bruker • Avance 400 MHZ
Equipped with an ATM-BBO probe and a BACS-60 auto sampler.
Mass spectrometer • Waters • Q-ToF micro
Equipped with an ElectroSpray ionisation probe and an Alliance HPLC system.
DNA synthesizer • K&A Laborgeräte • H-8 DNA synthesizer
The H-8 DNA synthesizer is a robust DNA synthesizer that allows for the parallel synthesis of up to 8 DNA, RNA, and modified nucleic acids. In addition, the synthesizer has a low consumption of […]
Akta HPLC • GE Healthcare • Akta Pure
Our HPLC system is dedicated to the purification of modified oligonucleotides and nucleoside triphosphates. The purifications are usually carried out by anion exchange chromatography (analytical column from GE Healthcare) and reversed-phase HPLC (analytical and […]
Fundings
Publications
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2019Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs, Methods Mol. Biol. 2019, 1973, 1-13.
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2019Chemical methods for the modification of RNA, Methods 2019, 161, 64-82.
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2019Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids, ChemBioChem 2019, 20, 860 – 871.
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2019Towards the enzymatic formation of artificial metal base pairs with a carboxy-imidazole-modified nucleotide, J. Inorg. Biochem. 2019, 191, 154-163.
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2018Incorporation of a minimal nucleotide into DNA, Tetrahedron Lett. 2018, 59, 4241-4244.
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2018Tetrahedral DNAzymes for enhanced intracellular gene-silencing activity, Chem. Commun., 2018,54, 9410-9413 .
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2018Shaping Rolling Circle Amplification Products into DNA Nanoparticles by Incorporation of Modified Nucleotides and Their Application to In Vitro and In Vivo Delivery of a Photosensitizer, Molecules 2018, 23, 1833.
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2018Applications of Ruthenium Complexes Covalently Linked to Nucleic Acid Derivatives, Molecules, 2018, 23, 1515.
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2018Aptamer chemistry, Adv. Drug Deliv. Rev. 2018, 134, 3–21.
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2018DNA Synthesis by Primer Exchange Reaction Cascades, ChemBioChem, 2018, 19, 422-424.
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Contact
25-28 Rue du Docteur Roux 75015, Paris France email: marcel.hollenstein@pasteur.fr Phone: +33 (0)1 44 38 94 66