The research interests of the laboratory for Bioorganic Chemistry of Nucleic acids reside in the synthesis of activated building blocks of chemically modified nucleoside analogs for their use in selection experiments for the crafting of functional nucleic acids with enhanced activities. Particularly, we strive to generate modified aptamers to be used as tools for medical imaging applications and the detection of specific targets such as enzymes, peptides, cancer cells, and small molecules. In addition, we are developing catalytic nucleic acids for the detection of DNA lesions as well as for their use as synthetic tools in molecular biology.
Field Test for Rabies Diagnostic (FiTeRaD)
The FiTeRaD (Field Tests for Rabies Diagnostic) project aims to develop and validate, in laboratory and in the field, the first point of care tests (POCT) for the rapid detection of the etiological agent […]
Expansion of the genetic alphabet with metal base pairs
Expanding the genetic code beyond the A-T/G-C Watson-Crick canonical base pairs and 20 amino acids of natural organisms is a long standing goal in synthetic biology. Reprogrammation of the genetic code can lead to […]
Therapeutic tools based on nucleic acids
An important research axis of the laboratory is to modify nucleic acids with chemical groups in order to improve their therapeutic usefulness. Particularly, modified aptamers (i.e. oligonucleotides capable of binding to targets with high […]
Synthesis of chemically modified nucleoside triphosphates
Functional nucleic acids (aptamers, DNAzymes, ribozymes, and aptazymes) are generated by in vitro Darwinian evolution methods (SELEX and related methods of in vitro selection). We are interested to expand the capacity of these functional […]
Equipped with an ATM-BBO probe and a BACS-60 auto sampler.
Equipped with an ElectroSpray ionisation probe and an Alliance HPLC system.
The H-8 DNA synthesizer is a robust DNA synthesizer that allows for the parallel synthesis of up to 8 DNA, RNA, and modified nucleic acids. In addition, the synthesizer has a low consumption of […]
Our HPLC system is dedicated to the purification of modified oligonucleotides and nucleoside triphosphates. The purifications are usually carried out by anion exchange chromatography (analytical column from GE Healthcare) and reversed-phase HPLC (analytical and […]
2020Enzymatic Formation of an Artificial Base Pair Using a Modified Purine Nucleoside Triphosphate, ACS Chem. Biol. 2020, 15, 2872-2884.
2020Enzymatic Construction of Artificial Base Pairs: The Effect of Metal Shielding, ChemBioChem 2020, 21, 3398-3409.
2020Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker., Proc. Natl. Acad. Sci. U.S.A. 2020 Jul; (): .
2020Stealth fluorescence labeling for live microscopy imaging of mRNA delivery, BioxRiv, 2020, 172767 (doi: 10.1101/2020.07.01.172767).
2020Orthogonal Genetic Systems, ChemBioChem 2020, 21, 1408-1411.
2020Enzymatic synthesis of biphenyl-DNA oligonucleotides, Biorg. Med. Chem. 2020, 28, 115487.
2020Self‐Assembly of DNA and RNA Building Blocks Explored by Nitrogen‐14 NMR Crystallography: Structure and Dynamics, ChemPhysChem 2020, 21, 1044-1051.
2020Ruthenium-initiated polymerization of lactide: a route to remarkable cellular uptake for photodynamic therapy of cancer, Chem. Sci., 2020, 11, 2657-2663.
2019Enzymatic Formation of an Artificial Base Pair Using a Modified Adenine Nucleoside Triphosphate, ChemRxiv 2019, doi: 10.26434/chemrxiv.11427459.v1.
2019On the Enzymatic Formation of Metal Base Pairs with Thiolated and pKa-Perturbed Nucleotides, ChemBioChem 2019, 20, 3032 – 3040.
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