The research interests of the laboratory for Bioorganic Chemistry of Nucleic acids reside in the synthesis of activated building blocks of chemically modified nucleoside analogs for their use in selection experiments for the crafting of functional nucleic acids with enhanced activities. Particularly, we strive to generate modified aptamers to be used as tools for medical imaging applications and the detection of specific targets such as enzymes, peptides, cancer cells, and small molecules. In addition, we are developing catalytic nucleic acids for the detection of DNA lesions as well as for their use as synthetic tools in molecular biology.
Expansion of the genetic alphabet with metal base pairs
Expanding the genetic code beyond the A-T/G-C Watson-Crick canonical base pairs and 20 amino acids of natural organisms is a long standing goal in synthetic biology. Reprogrammation of the genetic code can lead to […]
Therapeutic tools based on nucleic acids
An important research axis of the laboratory is to modify nucleic acids with chemical groups in order to improve their therapeutic usefulness. Particularly, modified aptamers (i.e. oligonucleotides capable of binding to targets with high […]
Synthesis of chemically modified nucleoside triphosphates
Functional nucleic acids (aptamers, DNAzymes, ribozymes, and aptazymes) are generated by in vitro Darwinian evolution methods (SELEX and related methods of in vitro selection). We are interested to expand the capacity of these functional […]
Equipped with an ATM-BBO probe and a BACS-60 auto sampler.
Equipped with an ElectroSpray ionisation probe and an Alliance HPLC system.
The H-8 DNA synthesizer is a robust DNA synthesizer that allows for the parallel synthesis of up to 8 DNA, RNA, and modified nucleic acids. In addition, the synthesizer has a low consumption of […]
Our HPLC system is dedicated to the purification of modified oligonucleotides and nucleoside triphosphates. The purifications are usually carried out by anion exchange chromatography (analytical column from GE Healthcare) and reversed-phase HPLC (analytical and […]
2019Compatibility of 5-ethynyl-2’F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers, Org. Biomol. Chem. 2019, 17, 8083 - 8087.
2019Nucleic acid enzymes based on functionalized nucleosides, Curr. Opin. Chem. Biol. 2019, 52, 93-101.
2019Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs, Methods Mol. Biol. 2019, 1973, 1-13.
2019Chemical methods for the modification of RNA, Methods 2019, 161, 64-82.
2019Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids, ChemBioChem 2019, 20, 860 – 871.
2019Towards the enzymatic formation of artificial metal base pairs with a carboxy-imidazole-modified nucleotide, J. Inorg. Biochem. 2019, 191, 154-163.
2018Incorporation of a minimal nucleotide into DNA, Tetrahedron Lett. 2018, 59, 4241-4244.
2018Tetrahedral DNAzymes for enhanced intracellular gene-silencing activity, Chem. Commun., 2018,54, 9410-9413 .
2018Shaping Rolling Circle Amplification Products into DNA Nanoparticles by Incorporation of Modified Nucleotides and Their Application to In Vitro and In Vivo Delivery of a Photosensitizer, Molecules 2018, 23, 1833.
2018Applications of Ruthenium Complexes Covalently Linked to Nucleic Acid Derivatives, Molecules, 2018, 23, 1515.
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