Marcel Hollenstein, hailing from Lutry (Switzerland), studied chemistry at the University of Lausanne (Switzerland) where he obtained a diploma in 1999. He then moved to the group led by Prof. Christian Leumann at the University of Bern (Switzerland) for his PhD, obtained in 2004. During his PhD, he synthesized fluorinated peptide nucleic acids (PNA) with the aim of investigating structural key features of these important DNA analogs. In 2005, he moved to the group of Prof. David Perrin at the University of British Columbia (Vancouver, Canada) for a postdoctoral stay. During this time, he was involved in Darwinian selection experiments (SELEX) to generate chemically modified DNA enzymes capable of cleaving RNA targets in the absence of divalent metal co-factors. In 2009, Marcel moved back to Bern (in the group of Prof. Christian Leumann) with a grant from the Swiss National Science Foundation (Ambizione fellowship) to work on independent research projects that dealt with the synthesis and biochemical characterization of modified nucleoside triphosphates for their futher use in the development of aptamers, DNA enzymes, and bionanomaterials. In January 2016, he was offered a position at the Institut Pasteur (Paris, France) to lead a 5-year junior group ‘bioorganic chemistry of nucleic acids’ in the department of structural biology and chemistry.
INCEPTION – Institut Convergence for the study of Emergence of Pathology Through Individuals and Populations
IINCEPTION Goal The Inception’s goal is to develop a core structure to mobilize data resources, numerical sciences, and fundamental experimental biology in a range of health issues (Official website here : https://www.inception-program.fr/en). Inception program […]
Field Test for Rabies Diagnostic (FiTeRaD)
The FiTeRaD (Field Tests for Rabies Diagnostic) project aims to develop and validate, in laboratory and in the field, the first point of care tests (POCT) for the rapid detection of the etiological agent […]
Expansion of the genetic alphabet with metal base pairs
Expanding the genetic code beyond the A-T/G-C Watson-Crick canonical base pairs and 20 amino acids of natural organisms is a long standing goal in synthetic biology. Reprogrammation of the genetic code can lead to […]
Therapeutic tools based on nucleic acids
An important research axis of the laboratory is to modify nucleic acids with chemical groups in order to improve their therapeutic usefulness. Particularly, modified aptamers (i.e. oligonucleotides capable of binding to targets with high […]
Synthesis of chemically modified nucleoside triphosphates
Functional nucleic acids (aptamers, DNAzymes, ribozymes, and aptazymes) are generated by in vitro Darwinian evolution methods (SELEX and related methods of in vitro selection). We are interested to expand the capacity of these functional […]
2021Stealth Fluorescence Labeling for Live Microscopy Imaging of mRNA Delivery, J. Am. Chem. Soc. 2021, 143, 5413−5424.
2020Enzymatic Formation of an Artificial Base Pair Using a Modified Purine Nucleoside Triphosphate, ACS Chem. Biol. 2020, 15, 2872-2884.
2020Enzymatic Construction of Artificial Base Pairs: The Effect of Metal Shielding, ChemBioChem 2020, 21, 3398-3409.
2020Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker., Proc. Natl. Acad. Sci. U.S.A. 2020 Jul; (): .
2020Orthogonal Genetic Systems, ChemBioChem 2020, 21, 1408-1411.
2020Enzymatic synthesis of biphenyl-DNA oligonucleotides, Biorg. Med. Chem. 2020, 28, 115487.
2020Self‐Assembly of DNA and RNA Building Blocks Explored by Nitrogen‐14 NMR Crystallography: Structure and Dynamics, ChemPhysChem 2020, 21, 1044-1051.
2020Ruthenium-initiated polymerization of lactide: a route to remarkable cellular uptake for photodynamic therapy of cancer, Chem. Sci., 2020, 11, 2657-2663.
2019Enzymatic Formation of an Artificial Base Pair Using a Modified Adenine Nucleoside Triphosphate, ChemRxiv 2019, doi: 10.26434/chemrxiv.11427459.v1.
2019On the Enzymatic Formation of Metal Base Pairs with Thiolated and pKa-Perturbed Nucleotides, ChemBioChem 2019, 20, 3032 – 3040.
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