Eukaryotic cells contain extensive internal membranes defining many compartments having each specific functions. However these organelles are constantly moving and reshaping, still retaining their identity. The maintenance of cell compartmentalization in eukaryotes is thus very complex and tightly controlled. Nathalie Sauvonnet’s team deals with intracellular trafficking of eukaryotic cells and how it regulates cell and tissue organization. In particular we study endocytosis and exocytosis pathways and their linked to lipid distribution, cell compartmentalization and host-pathogen interactions. For this purpose we used an array of techniques from high resolution microscopy, single molecules tracking, robust image analysis, statistical classification, crispR-cas9 edition to organ-on-a-chip.
Endocytosis: the clathrin-independent uptake of cytokine receptors
Endocytosis is an essential process used to internalize a wide range of molecules, and involved in many processes such as cell migration, cell division, immunity and pathogen invasion. Several endocytic pathways exist, enabling the […]
Exocytosis: inhibition of secretion/recycling by the pathogen Shigella
The distribution of cholesterol – in a gradient – is essential for compartments organization. Cholesterol distribution within the organelles should be tightly controlled but so far little is known on the dynamics its intracellular […]
2018Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics, Nat Commun 2018 Feb;9(1):698.
2018Revisiting absorption and electronic circular dichroism spectra of cholesterol in solution: a joint experimental and theoretical study, Phys Chem Chem Phys 2018 Feb;20(7):5274-5284.
2018Quantitative and Statistical Study of the Dynamics of Clathrin-Dependent and -Independent Endocytosis Reveal a Differential Role of EndophilinA2, Cell Rep 2018 Feb;22(6):1574-1588.
2015Membrane protrusion powers clathrin-independent endocytosis of interleukin-2 receptor, EMBO J. 2015 Aug;34(16):2147-61.
2015Statistical analysis of molecule colocalization in bioimaging, Cytometry A 2015 Jun;87(6):568-79.
2014Endophilin marks and controls a clathrin-independent endocytic pathway, Nature 2015 Jan;517(7535):460-5.
2014Membrane trafficking. Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling, Science 2014 Jun;344(6191):1510-5.
2014Targeting spare CC chemokine receptor 5 (CCR5) as a principle to inhibit HIV-1 entry, J. Biol. Chem. 2014 Jul;289(27):19042-52.
2013Analysis of the spatial organization of molecules with robust statistics, PLoS ONE 2013;8(12):e80914.
2013Phosphoinositide 3-kinase at the crossroad between endocytosis and signaling of cytokine receptors, Commun Integr Biol 2013 Jul;6(4):e24243.
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