Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
22
Nov 2019
Status
Ongoing
Members
6
Structures
5

About

The intestine, and in particular the colon, comprising a mucus blanket, an epithelial monolayer and the lamina propria rich in immune cells, is constantly subjected to shear stress (flow) and peristaltic motion (stretching), both forces being important for the tissue organization and renewal. Altogether these cells layers cooperate to form a homogeneous and protective barrier facing the external side (from the lumen). This complex and heterogeneous microenvironment generates a wealth of “ecological niches” permanently exposed to microorganisms that can be beneficial (microbiota) or damageable (enteric pathogens). As a result, intestinal homeostasis is very complex and often invalidated, leading to a long list of enteric diseases, ranging from inflammatory, cancer, to infectious diseases, which are still far from being understood at the tissue level. In this project, we aim to decipher the impact of mechanical forces on the integrity of the gut barrier.

-Gut-on-chip to recapitulate human intestine physiology (3D and mechanical stimulation).

-Use of gut-on-chips to investigate host-pathogen interactions with several microbes: Shigella, Amoeba, Norovirus, Cryptosporidium to better chacarteirze infectious process at the tissue level and replace animal experimentation.

-Building a computational framework able to follow the dynamics (in space and time) of intestinal infections in 3D tissues with Intestine-Chips and  characterizing the effect of mechanical forces on plasma membrane stiffness or on intercellular junction integrity (PTR232-2019, collaboration with E. Labruyère-JC Olivo-Marin).

-Human colon tissues to generate colon-on-chip (from colonoscopy, personalised medicine, Collaboration with G. Nigro).

-Use of colon-on-chip to unravel colorectal cancer (CRC) with Gold NanoParticules (GNPs): in collaboration with Torskal that produces GNPs we aim at recapitulating a colon having healthy and cancers cells from patients to study the endocytosis of GNPs in cancer cells and then destroy specifically cancer cells by photothermal therapy or irradiation.

Associated Publication:

Grassart A, Malardé V, Gobaa S, Sartori-Rupp A, Kerns J, Karalis K, Marteyn B, Sansonetti PJ and N Sauvonnet. Bioengineered Human Organ-on-Chip Reveals Intestinal Microenvironment and Mechanical Forces Impacting Shigella Infection. Cell Host and Microbe. 2019, Sep 11;26(3):435-444.

Fundings