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Présentation
Services
Booking siteCB UTechS proposes various services to the community :
- Training for autonomous use of the equipment
- Assisted use for a selection of technologies
- Advice and technical support
- Development of new of applications and pipelines
- Scientific collaborations
- Access to different data analysis software and project-tailored bioinformatics support for analysis of large-scale data generated by our technologies
Contact : CB_UTechS@pasteur.fr for more information.
Training @ Teaching
TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]
Use Fees
Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below : Technology Autonomous Assisted Training* Academic / Industry Cytometers […]
Équipements
Booking siteCB UTechS is an integrated technology platform for multidimensional cell phenotyping & molecular profiling. We propose single-cell cutting-edge technologies and data analysis pipelines. All technologies are bookable through the PPMS online equipment and training reservation site. The equipment is installed in six dedicated laboratories, with a significant part of the technologies situated within BSL2+ (biosafety level 2 +) environment allowing processing of human and infectious material. View a non-exhaustive list of pathogens currently manipulated in the laboratory.
Laboratories and equipment location
Cell phenotyping and sorting (Flow Cytometry Platform)
- – 3 Conventional cytometers (Symphony A5, LSRFortessa, Cytoflex for simultaneous detection of up to 28 parameters
- – Spectral cytometer (ID7000) for indept phenotyping (>40 parameters) and management of autofluorescence
- – Imaging cytometer MARKII (Luminex): spatial quantification of intra- and inter-cellular processes in cells in suspension
- – 7 Cell sorters (2 Astrios, 4 FACSAria Fusion, 1 FACSAria S6): simultaneous sorting of ≤ 6 cell populations, using ≤28 fluorophores in a single panel. 2 sorters available in Bsl2+ environment for sorting of human and/or infectious material.
- – 2 Magnetic-based cell sorters (AutoMACS): pre-enrichment of rare cell populations.
- – MultiMACS: isolation of targeted cell populations from whole blood.
Cell culture and functional assays in Bsl2+ environment (Biomarkers Platform)
- Fully-equipped cell culture facility
- Hypoxia cabinet XVivo (Biospherix): manipulation and cell culture under controlled O2, CO2, N2, temperature and humidity
- Seahorse (Agilent): measuring cellular glycolysis and mitochondrial respiration under controlled O2, CO2, N2, temperature and humidity
- Incucyte S6 (Sartorius): short- and long-term live-content imaging in any conventional cell culture support
- Amaxa nucleofector (Lonza): nucleofection
Multiplex and ultrasensitive protein quantification (immunoassays) (Biomarkers Platform)
- Bioplex 200 (Biorad) coupled to DropArray (Curiox): quantification of ≤ 200 analytes in cell lysis, supernatants or body-fluids with pM sensitivity from reduced sample (5uL) and reagent volumes.
- Simoa HD-x (Quanterix): digital ELISA for quantification of proteins with fM sensitivity
- SP-X (Quanterix): ultrasensitive multiplex protein detection (10-plex)
- Isolight (Isoplexis): single -cell sectretome analysis (32-plex)
RNA/DNA profiling in bulk (Single Cell Platform)
- Nanostring (Nanostring): direct multiplex RNA quantification (800-plex)
- Bioanalyzer (Agilent): quality control of DNA, RNA, & proteins
- Qubit: RNA-quantification
Multi-modal single cell profiling (Single Cell Platform)
- 10xGenomics (3 instruments): single-cell transcriptomics, single-nuclei transcriptomics CITE-Seq, ATAC-Seq, TCR/BCR rearrangement. Available in Bsl2+ confinement for analysis of human and/or infectious cells.
- Spatial transcriptomics (10xGenomics)
- MARS-Seq
- Biomark (Fluidigm): multiplex single cell real-time PCR (96-plex)
Data analysis
- Schnapps –shiny application for analysis of scRNA-Seq data by biologists
- FLowJo (BD) – a tool of choice for analysis of multidimensional flow cytometry data
- Qlucore- user-friendly PCA tool for analysis of high-dimensional data
NanoFCM • NanoFCM Co. Ltd •
The NanoAnalyzer is a dedicated flow cytometer built specifically for analyzing nano-sized particles such as extracellular vesicles (EVs), viruses, nanoparticles, based on light scattering and fluorescence for the detection of single virions or EVs […]
2 • Spectral Analysers • Sony •
ID 7000 6 lasers (325nm, 355nm, 405nm, 488nm, 561nm and 637nm) which allow for full spectral detection of the resulting emission spectral. BSL2+ : P2+ pathogens and blood ID 7000 Optical Configuration
CytoFlex S – Cytometer • Beckman Coulter •
4 lasers (405nm, 488nm, 561nm, 638nm) and 13 fluorescent detectors. This analyser is under a P2 PSM : P2 pathogens and Blood plate analysis Software : CytExpert CytoFlex S Optical Configuration
LSR Fortessa – Cytometer • BD •
5 laser (355nm, 405nm, 488nm, 561nm and 640nm) and 18 fluorescent detectors No pathogens – No Blood Software : Diva 8 LSR Fortessa Optical Configuration BD LSR Fortessa cytometer technical poster showing optical […]
Symphony A5 – Cytometer • BD •
5 laser (355nm, 405nm, 488nm, 561nm and 637nm) and 31 fluorescent detectors No pathogens – No Blood Software : Diva 8 Web site BD FACSymphony : Symphony A5 Flow Cytometer FACSymphony A5 Optical Configuration […]
Symphony S6 – Cell sorter • BD •
5 lasers (355nm, 405nm, 488nm, 561nm, and 637nm) and 29 fluorescent detectors BSL2+ : P2+ pathogens and blood Nozzle sizes : 70um; 85um; 100um; 130um Sorting : 1 to 6 way for tubes and […]
FACSAria Fusion – Cell sorters • BD •
The BD FACSAria™ Fusion Flow Cytometer provides sensitivity for multicolor and sorting applications. The fusion of safety, performance and sorting The BD FACSAria™ Fusion Flow Cytometer combines sorting capabilities with best-in-class biosafety expertise […]
3 • FACSAria III – Cell sorters • BD •
BD FACSAriaIII™ sorters Family Those instruments can be used by independent researchers or as an assisted service. We propose access to 3 FACSAria III cell sorters for bulk or single-cell sorting. BD web site : Technical […]
2 • Astrios High Speed Sorter • Beckman Coulter • MoFlo EQ
The MoFlo Astrios is created by Beckman Coulter’s compagny. This is a jet-in-the-air sorter and has 5 or 7 lasers (UV, violet, red, blue and yellow-green) and two light scatter parameter can be measured […]
Seahorse – Cell metabolism • •
Seahorse Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- […]
Incucyte – Live cell imaging • •
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]
BioPlex 200 – protein quantification • •
BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]
Ion Proton & PGM – Next Generation Sequencing • •
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]
2 • nCounter NanoString – Gene expression profiling • •
nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]
Fluidigm C1 – single-cell profiling • •
Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]
BioMark – gene expression profiling • •
BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]
2100 Bioanalyzer – RNA and DNA quality/quantity • •
Bioanalyzer Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.
Qubit 2.0 – DNA/RNA quantification • •
Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
2 • AutoMACS Pro • Miltenyi Biotec •
Rapid magnetic enrichment or depletion of cell populations Easy cell separation with the autoMACS® Pro Separator. Reduce your manual handling steps, save time, and count on greater reliability, reproducibility, and standardization. The autoMACS® Pro […]
ImageStreamX – Imaging cytometry • •
ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
Membres
Milena Hasan
Responsable de Structure
Responsable
Groupes
Platform
Biomarqueurs
Milena Hasan
Platform
Plateforme de Cytométrie
Sophie Novault
Group
Group : Milieu Intérieur
Darragh Duffy
Platform
Plate-Forme d’Immuno-monitoring
Milena Hasan
Platform
Plateforme Single Cell
Valentina Libri
Cours
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
2022-06-30 09:00:00
2022-07-06 18:00:00
Europe/Paris
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
28 Rue du Docteur Roux, Paris, France
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
2018-12-02 09:00:00
2018-12-15 18:00:00
Europe/Paris
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
HKU-Pasteur Research Centre, Hong Kong
Projets
Projet Transversal
Outils
Financements
Publications
Télécharger-
2023Standardized high-dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies., Cytometry A 2023 Sep; (): .
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2023Dissecting human population variation in single-cell responses to SARS-CoV-2, Nature, 2023, 53 (8), pp.e2250307. ⟨10.1038/s41586-023-06422-9⟩.
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2023T cell migration and effector function differences in familial adenomatous polyposis patients with APC gene mutations, Frontiers in Immunology, 2023, 14, pp.1163466. ⟨10.3389/fimmu.2023.1163466⟩.
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2023Single-cell transcriptomic profiling of the mouse cochlea: An atlas for targeted therapies., Proc Natl Acad Sci U S A 2023 Jun; 120(26): e2221744120.
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2023Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans., mBio 2023 Jun; (): e0087023.
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2023Biological differences between FIM2 and FIM3 fimbriae of Bordetella pertussis: not just the serotype, Microbes and Infection, 2023, pp.105152. ⟨10.1016/j.micinf.2023.105152⟩.
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2023In vitro cellular reprogramming to model gonad development and its disorders., Sci Adv 2023 Jan; 9(1): eabn9793.
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2022Defective activation and regulation of type I interferon immunity is associated with increasing COVID-19 severity., Nat Commun 2022 Nov; 13(1): 7254.
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2022IRF8 regulates efficacy of therapeutic anti-CD20 monoclonal antibodies., Eur J Immunol 2022 Aug; (): .
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2022Putative Biomarkers of Environmental Enteric Disease Fail to Correlate in a Cross-Sectional Study in Two Study Sites in Sub-Saharan Africa., Nutrients 2022 Aug; 14(16): .
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+Voir la liste complète de publications
Contact
Phone: 01.40.61.30.27. Email: crt-tc@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France