The Technical Core of the Center for Translational Science (CRT) of the Institut Pasteur provide access to cutting edge technologies selected to support translational research. Supported by the Centre d’Immunologie Humaine (CIH) and the Cytometry Platform (PFC), the Core permits cell sorting, cell phenotyping, single cell profiling & sequencing.
CB UTechS proposes to the community: (1) training for autonomous use of the equipment; (2) assisted use for a selection of technologies (cell sorters, cytometers); (3) advice and technical support; (4) development of new of applications and pipelines; (5) scientific collaborations. Through (5) training and access to different data analysis software, as well as through an adapted bioinformatics support we aim to facilitate the analysis of large-scale data generated by our technologies.
TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]
Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below: Technology Autonomous Assisted Training* IP + Academic / Industry […]
CB UTechS is an integrated technology platform for multidimensional cell phenotyping & molecular profiling. We propose single-cell cutting-edge technologies and data analysis pipelines. All technologies are bookable through the PPMS online equipment and training reservation site. The equipment is installed in six dedicated laboratories, with a significant part of the technologies situated within BSL2+ (biosafety level 2 +) environment allowing processing of human and infectious material. View a non-exhaustive list of pathogens currently manipulated in the laboratory.
Laboratories and equipment location
Customized solutions for high parameter cell analysis The BD FACSymphony™ system is a novel cell analyzer that leverages the inherent benefits of flow cytometry and enables the simultaneous measurement of up to 30 different characteristics […]
Seahorse Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- […]
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]
BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]
nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]
Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]
BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]
Bioanalyzer Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.
Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
Rapid magnetic enrichment or depletion of cell populations Easy cell separation with the autoMACS® Pro Separator. Reduce your manual handling steps, save time, and count on greater reliability, reproducibility, and standardization. The autoMACS® […]
We propose access to 3 FACSAria III cell sorters for bulk or single-cell sorting: Technical Specifications PINK PANTHER 4 lasers (405nm, 488nm, 561nm and 640nm), with “index sorting” (FACS DIVA 8 software) […]
Charlie Brown 5 lasers (355nm, 405nm, 488nm, 561nm and 640nm), with “index sorting” (software FACSDiva 7) Configuration Charlie Brown Build your panel with the instrument configuration: FACS ARIA II Charlie Brown This FACSAria sorter is located […]
Spectral analyzer SP6800 – Sony Emerging technology that revolutionizes the cytometry by identifying specific fluorochrome “signatures”and by eliminating need for compensation. Equipped by 3 lasers (488nm, 635nm and 405nm), SP6800 collects the entire fluorescence spectrum […]
Equipped with five laser lines (355nm 405nm, 488nm, 561nm and 640nm) and 18 fluorescent detectors BD FACS Diva 6.2 software Easy tool to build your panels with the configuration of your instrument See: BD Fortessa […]
Equipped with four laser lines (355nm, 405nm, 488nm and 640nm), 17 fluorescent detectors and an HTS unit for high-throughput screening in plates
ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
Plateforme de Cytométrie
Group : Milieu Intérieur
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
Milieu Intérieur: Deconvolution of the healthy immune response
Systems approaches for the study of immune signaling pathways have been traditionally based on selected purified cell types or cultured cell lines. However, in vivo responses involve the coordinated action of multiple cell types, […]
Projet de Recherche Clinique : Impact des mutations du gène APC (Adenomatous polyposis coli) dans les réponses immunes anti-tumorales
La polypose adénomateuse familiale est une maladie caractérisée par l’apparition dans l’intestin de nombreux polypes susceptibles d‘évoluer en cancer en l’absence de prise en charge. Il s’agit d’une maladie génétique rare, le plus souvent […]
1,000 healthy donors
The Milieu Intérieur Consortium has established a 1000-person healthy population-based study (stratified according to sex and age), creating an unparalleled opportunity for assessing the determinants of human immunologic variance. The Milieu Intérieur Consortium initiated […]
Understanding protective humoral immunity in asymptomatic dengue-infected individuals
Dengue is caused by dengue virus (DENV), a virus which can be transmitted by Aedes mosquitos. Half of the world’s population live in areas where the mosquito and the virus are circulating. Infection by […]
CoSImmGEn-F : constitution d’une cohorte de sujets atteints de polypose adénomateuse familiale et porteurs d’une mutation du gène APC afin d’étudier le rôle de la génétique du système immunitaire et de l’environnement dans la maladie
Rationnel La polypose adénomateuse familiale se manifeste par l’apparition dans l’intestin de nombreuses excroissances dénommées polypes adénomateux et susceptibles d‘évoluer en cancers en l’absence de prise en charge. Il s’agit d’une maladie génétique, le […]
Deciphering dormancy in Cryptocococcus neoformans
Cryptococcus neoformans is a sugar-coated yeast that is able to interact closely with numerous organisms in the environment including amebae, paramecium of nematodes. The interaction with these organisms probably shaped its virulence. The ability […]
SINGLE CELL RESOURCES AT INSTITUT PASTEUR
Désolé, cet article est seulement disponible en Anglais Américain.
2021Gut microbiome stability and dynamics in healthy donors and patients with non-gastrointestinal cancers., J Exp Med 2021 Jan; 218(1): .
2020Transcriptome and epigenome diversity and plasticity of muscle stem cells following transplantation., PLoS Genet 2020 Oct; 16(10): e1009022.
2020Angiopoietin-2 as a marker of endothelial activation is a good predictor factor for intensive care unit admission of COVID-19 patients., Angiogenesis 2020 Nov;23(4):611-620.
2019Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis, Nat Commun 2019 04;10(1):1929.
2019Histamine releasing factor and elongation factor 1 alpha secreted via malaria parasites extracellular vesicles promote immune evasion by inhibiting specific T cell responses., Cell. Microbiol. 2019 07; 21(7): e13021.
2018Human thymopoiesis is influenced by a common genetic variant within the TCRA-TCRD locus., Sci Transl Med 2018 09; 10(457): .
2018Identifying the etiology and pathophysiology underlying stunting and environmental enteropathy: study protocol of the AFRIBIOTA project, BMC Pediatr 2018 Jul;18(1):236.
2018Primary antiphospholipid syndrome and antiphospholipid syndrome associated to systemic lupus: Are they different entities?, Autoimmun Rev 2018 Aug;17(8):739-745.
2017Role of the IL-12/IL-35 balance in patients with Sjögren syndrome, J. Allergy Clin. Immunol. 2018 Jul;142(1):258-268.e5.
2017Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry, J Vis Exp 2017 May;(123).
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