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Services

Booking site

CB UTechS proposes various services to the community:

  • Training for autonomous use of the equipment
  • Assisted use for a selection of technologies
  • Advice and technical support
  • Development of new of applications and pipelines
  • Scientific collaborations
  • Access to different data analysis software and a project-tailored
    bioinformatics support for analysis of large-scale data generated by our technologies

Contact: CB_UTechS@pasteur.fr for more information

  • xxxx
  • xxxx

Training @ Teaching

TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]

Use Fees

Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below: Technology Autonomous Assisted Training* Academic / Industry Cytometers & […]

Équipements

Booking site

CB UTechS is an integrated technology platform for multidimensional cell phenotyping & molecular profiling. We propose single-cell cutting-edge technologies and data analysis pipelines. All technologies are bookable through the PPMS online equipment and training reservation site. The equipment is installed in six dedicated laboratories, with a significant part of the technologies situated within BSL2+ (biosafety level 2 +) environment allowing processing of human and infectious material. View a non-exhaustive list of pathogens currently manipulated in the laboratory.

Laboratories and equipment location

Cell phenotyping and sorting (Flow Cytometry Platform)

  • 3 Conventional cytometers (Symphony A5, LSRFortessa, Cytoflex for simultaneous detection of up to 28 parameters
  • Spectral cytometer (ID7000) for indept phenotyping (>40 parameters) and management of autofluorescence
  • Imaging cytometer MARKII (Luminex): spatial quantification of intra- and inter-cellular processes in cells in suspension
  • 7 Cell sorters (2 Astrios, 4 FACSAria Fusion, 1 FACSAria S6): simultaneous sorting of ≤ 6 cell populations, using ≤28 fluorophores in a single panel. 2 sorters  available in Bsl2+ environment for sorting of human and/or infectious material.
  • 2 Magnetic-based cell sorters (AutoMACS): pre-enrichment of rare cell populations.
  • MultiMACS: isolation of targeted cell populations from whole blood.

Cell culture and functional assays (in Bsl2+ environment)

  • Fully-equipped cell culture facility
  • Hypoxia cabinet XVivo (Biospherix): manipulation and cell culture under controlled O2, CO2, N2, temperature and humidity
  • Seahorse (Agilent): measuring cellular glycolysis and mitochondrial respiration under controlled O2, CO2, N2, temperature and humidity
  • Incucyte S6 (Sartorius): short- and long-term live-content imaging in any conventional cell culture support
  • Amaxa nucleofector (Lonza): nucleofection

Multiplex and ultrasensitive protein quantification (immunoassays)

  • Bioplex 200 (Biorad) coupled to DropArray (Curiox): quantification of ≤ 200 analytes in cell lysis, supernatants or body-fluids with pM sensitivity from reduced sample (5uL) and reagent volumes.
  • Simoa HD-x (Quanterix): digital ELISA for quantification of proteins with fM sensitivity
  • SP-X (Quanterix): ultrasensitive multiplex protein detection (10-plex)
  • Isolight  (Isoplexis): single -cell sectretome analysis (32-plex)

RNA/DNA profiling in bulk

  • Nanostring (Nanostring): direct multiplex RNA quantification (800-plex)
  • Bioanalyzer (Agilent): quality control of DNA, RNA, & proteins
  • Qubit: RNA-quantification

Multi-modal single cell profiling

  • 10xGenomics (3 instruments): single-cell transcriptomics, single-nuclei transcriptomics CITE-Seq, ATAC-Seq, TCR/BCR rearrangement. Available in Bsl2+ confinement for analysis of human and/or infectious cells.
    • Spatial transcriptomics (10xGenomics)
    • MARS-Seq
    • Biomark (Fluidigm): multiplex single cell real-time PCR (96-plex)

Data analysis

  • Schnapps –shiny application for analysis of scRNA-Seq data by biologists
  • FLowJo (BD) – a tool of choice for analysis of multidimensional flow cytometry data
  • Qlucore- user-friendly PCA tool for analysis of high-dimensional data

2 • Spectral Analysers • Sony •

ID 7000 6 lasers (355nm, 405nm, 488nm, 561nm and 637nm) which allow for full spectral detection of the resulting emission spectral. BSL2+ : P2+ pathogens and blood ID 7000 Optical Configuration SP 6800 3 […]

CytoFlex S – Cytometer • Beckman Coulter •

4  lasers (405nm, 488 nm, 561 nm, 638 nm) and 13 fluorescent detectors. This analyser is under a P2 PSM : P2 pathogens and Blood plate analysis Software : Summit CytoFlex S Optical Configuration  […]

LSR Fortessa – Cytometer • BD •

5 laser (355nm 405nm, 488nm, 561nm and 640nm) and 18 fluorescent detectors No pathogens – No Blood Software : Diva 8 LSR Fortessa Optical Configuration      BD LSR Fortessa cytometer technical poster showing optical […]

Symphony A5 – Cytometer • BD •

5 laser (355nm 405nm, 488nm, 561nm and 637nm) and 31 fluorescent detectors No pathogens – No Blood Software : Diva 8 Web site BD FACSymphony : Symphony A5 Flow Cytometer FACSymphony A5 Optical Configuration    […]

Symphony S6 – Cell sorter • BD •

5 lasers (355nm, 405nm, 488nm, 561, and 637nm) and 28 fluorescent detectors BSL2+ : P2+ pathogens and blood Nozzle sizes : 70um; 85um; 100um; 130um Sorting : 1 to 6 way for tubes and […]

2 • FACSAria Fusion – Cell sorters • BD •

  The BD FACSAria™ Fusion Flow Cytometer provides sensitivity for multicolor and sorting applications. The fusion of safety, performance and sorting The BD FACSAria™ Fusion Flow Cytometer combines sorting capabilities with best-in-class biosafety expertise […]

3 • FACSAria III – Cell sorters • BD •

BD FACSAriaIII™ sorters Family Those instruments can be used by independent researchers or as an assisted service. We propose access to 3 FACSAria III cell sorters for bulk or single-cell sorting. BD web site : Technical […]

2 • Astrios High Speed Sorter • Beckman Coulter • MoFlo EQ

The MoFlo Astrios is created by Beckman Coulter’s compagny. This is a jet-in-the-air sorter and has 5 or 7 lasers (UV, violet, red, blue and yellow-green) and two light scatter parameter can be measured […]

SP6800 – Spectral cytometer • Sony •

Spectral analyzer SP6800 – Sony Emerging technology that revolutionizes the cytometry by identifying specific fluorochrome “signatures”and by eliminating need for compensation. Equipped by 3 lasers (488nm, 635nm and 405nm), SP6800 collects the entire fluorescence spectrum […]

Seahorse – Cell metabolism • •

Seahorse Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- […]

Incucyte – Live cell imaging • •

Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]

BioPlex 200 – protein quantification • •

BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]

Amaxa – nucleofector • •

Nucleofection in 96 well shuttle or in cuvette

Ion Proton & PGM – Next Generation Sequencing • •

Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]

2 • nCounter NanoString – Gene expression profiling • •

nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]

Fluidigm C1 – single-cell profiling • •

Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]

BioMark – gene expression profiling • •

BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]

2100 Bioanalyzer – RNA and DNA quality/quantity • •

Bioanalyzer  Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.

Qubit 2.0 – DNA/RNA quantification • •

Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.

2 • AutoMACS Pro • Miltenyi Biotec •

Rapid magnetic enrichment or depletion of cell populations Easy cell separation  with the autoMACS® Pro Separator. Reduce your manual handling steps, save time, and count on greater reliability, reproducibility, and standardization. The autoMACS® Pro […]

ImageStreamX – Imaging cytometry • •

ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]

Membres

Cours

Single cell gene expression and beyond

Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]

2021-07-05 09:00:00 2021-07-09 17:00:00 Europe/Paris Single cell gene expression and beyond Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […] 28 Rue du Docteur Roux, Paris, France

10th HKU-Pasteur Immunology Course: December 2-15, 2018

  ‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]

2018-12-02 09:00:00 2018-12-15 18:00:00 Europe/Paris 10th HKU-Pasteur Immunology Course: December 2-15, 2018   ‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […] HKU-Pasteur Research Centre, Hong Kong

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Contact

Phone: 01.40.61.30.27. Email: crt-tc@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France