Désolé, cet article est seulement disponible en anglais américain.
Cliquez pour voir le graph
Connexions
Présentation
Services
Booking siteCB UTechS proposes various services to the community :
- Training for autonomous use of the equipment
- Assisted use for a selection of technologies
- Advice and technical support
- Development of new of applications and pipelines
- Scientific collaborations
- Access to different data analysis software and project-tailored bioinformatics support for analysis of large-scale data generated by our technologies
Contact : CB_UTechS@pasteur.fr for more information.
Guichet unique CRT
A new process for translational research project support is available. The CRT Opendesk, which was set up in 2017, is being replaced by the “Guichet Unique CRT”. This ensures optimal evaluation of projects according […]
Training @ Teaching
TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]
Use Fees
Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below : Technology Autonomous Assisted Training* Academic / Industry Cytometers […]
Équipements
Booking siteCB UTechS is an integrated technology platform for multidimensional cell phenotyping & molecular profiling. We propose single-cell cutting-edge technologies and data analysis pipelines. All technologies are bookable through the PPMS online equipment and training reservation site. The equipment is installed in six dedicated laboratories, with a significant part of the technologies situated within BSL2+ (biosafety level 2 +) environment allowing processing of human and infectious material. View a non-exhaustive list of pathogens currently manipulated in the laboratory.
Laboratories and equipment location
Cell phenotyping and sorting (Flow Cytometry Platform)
- – 3 Conventional cytometers (Symphony A5, LSRFortessa, Cytoflex for simultaneous detection of up to 28 parameters
- – Spectral cytometer (ID7000) for indept phenotyping (>40 parameters) and management of autofluorescence
- – Imaging cytometer MARKII (Luminex): spatial quantification of intra- and inter-cellular processes in cells in suspension
- – 7 Cell sorters (2 Astrios, 4 FACSAria Fusion, 1 FACSAria S6): simultaneous sorting of ≤ 6 cell populations, using ≤28 fluorophores in a single panel. 2 sorters available in Bsl2+ environment for sorting of human and/or infectious material.
- – 2 Magnetic-based cell sorters (AutoMACS): pre-enrichment of rare cell populations.
- – MultiMACS: isolation of targeted cell populations from whole blood.
Cell culture and functional assays in Bsl2+ environment (Biomarkers Platform)
- Fully-equipped cell culture facility
- Hypoxia cabinet XVivo (Biospherix): manipulation and cell culture under controlled O2, CO2, N2, temperature and humidity
- Seahorse (Agilent): measuring cellular glycolysis and mitochondrial respiration under controlled O2, CO2, N2, temperature and humidity
- Incucyte S6 (Sartorius): short- and long-term live-content imaging in any conventional cell culture support
- Amaxa nucleofector (Lonza): nucleofection
Multiplex and ultrasensitive protein quantification (immunoassays) (Biomarkers Platform)
- Bioplex 200 (Biorad) coupled to DropArray (Curiox): quantification of ≤ 200 analytes in cell lysis, supernatants or body-fluids with pM sensitivity from reduced sample (5uL) and reagent volumes.
- Simoa HD-x (Quanterix): digital ELISA for quantification of proteins with fM sensitivity
- SP-X (Quanterix): ultrasensitive multiplex protein detection (10-plex)
- Isolight (Isoplexis): single -cell sectretome analysis (32-plex)
RNA/DNA profiling in bulk (Single Cell Platform)
- Nanostring (Nanostring): direct multiplex RNA quantification (800-plex)
- Bioanalyzer (Agilent): quality control of DNA, RNA, & proteins
- Qubit: RNA-quantification
Multi-modal single cell profiling (Single Cell Platform)
- 10xGenomics (3 instruments): single-cell transcriptomics, single-nuclei transcriptomics CITE-Seq, ATAC-Seq, TCR/BCR rearrangement. Available in Bsl2+ confinement for analysis of human and/or infectious cells.
- Spatial transcriptomics (10xGenomics)
- MARS-Seq
- Biomark (Fluidigm): multiplex single cell real-time PCR (96-plex)
Data analysis
- Schnapps –shiny application for analysis of scRNA-Seq data by biologists
- FLowJo (BD) – a tool of choice for analysis of multidimensional flow cytometry data
- Qlucore- user-friendly PCA tool for analysis of high-dimensional data
NanoFCM • NanoFCM Co. Ltd •
The NanoAnalyzer is a dedicated flow cytometer built specifically for analyzing nano-sized particles such as extracellular vesicles (EVs), viruses, nanoparticles, based on light scattering and fluorescence for the detection of single virions or EVs […]
2 • Spectral Analysers • Sony •
ID 7000 6 lasers (325nm, 355nm, 405nm, 488nm, 561nm and 637nm) which allow for full spectral detection of the resulting emission spectral. BSL2+ : P2+ pathogens and blood ID 7000 Optical Configuration
CytoFlex S – Cytometer • Beckman Coulter •
4 lasers (405nm, 488nm, 561nm, 638nm) and 13 fluorescent detectors. This analyser is under a P2 PSM : P2 pathogens and Blood plate analysis Software : CytExpert CytoFlex S Optical Configuration
LSR Fortessa – Cytometer • BD •
5 laser (355nm, 405nm, 488nm, 561nm and 640nm) and 18 fluorescent detectors No pathogens – No Blood Software : Diva 8 LSR Fortessa Optical Configuration BD LSR Fortessa cytometer technical poster showing optical […]
Symphony A5 – Cytometer • BD •
5 laser (355nm, 405nm, 488nm, 561nm and 637nm) and 31 fluorescent detectors No pathogens – No Blood Software : Diva 8 Web site BD FACSymphony : Symphony A5 Flow Cytometer FACSymphony A5 Optical Configuration […]
Symphony S6 – Cell sorter • BD •
5 lasers (355nm, 405nm, 488nm, 561nm, and 637nm) and 29 fluorescent detectors BSL2+ : P2+ pathogens and blood Nozzle sizes : 70um; 85um; 100um; 130um Sorting : 1 to 6 way for tubes and […]
FACSAria Fusion – Cell sorters • BD •
The BD FACSAria™ Fusion Flow Cytometer provides sensitivity for multicolor and sorting applications. The fusion of safety, performance and sorting The BD FACSAria™ Fusion Flow Cytometer combines sorting capabilities with best-in-class biosafety expertise […]
3 • FACSAria III – Cell sorters • BD •
BD FACSAriaIII™ sorters Family Those instruments can be used by independent researchers or as an assisted service. We propose access to 3 FACSAria III cell sorters for bulk or single-cell sorting. BD web site : Technical […]
2 • Astrios High Speed Sorter • Beckman Coulter • MoFlo EQ
The MoFlo Astrios is created by Beckman Coulter’s compagny. This is a jet-in-the-air sorter and has 5 or 7 lasers (UV, violet, red, blue and yellow-green) and two light scatter parameter can be measured […]
Seahorse – Cell metabolism • •
Seahorse Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- […]
Incucyte – Live cell imaging • •
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]
BioPlex 200 – protein quantification • •
BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]
Ion Proton & PGM – Next Generation Sequencing • •
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]
2 • nCounter NanoString – Gene expression profiling • •
nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]
Fluidigm C1 – single-cell profiling • •
Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]
BioMark – gene expression profiling • •
BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]
2100 Bioanalyzer – RNA and DNA quality/quantity • •
Bioanalyzer Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.
Qubit 2.0 – DNA/RNA quantification • •
Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
2 • AutoMACS Pro • Miltenyi Biotec •
Rapid magnetic enrichment or depletion of cell populations Easy cell separation with the autoMACS® Pro Separator. Reduce your manual handling steps, save time, and count on greater reliability, reproducibility, and standardization. The autoMACS® Pro […]
ImageStreamX – Imaging cytometry • •
ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
Membres
Milena Hasan
Responsable de Structure
Responsable
Groupes
Platform
Biomarqueurs
Milena Hasan
Platform
Plateforme de Cytométrie
Sophie Novault
Group
Group : Milieu Intérieur
Darragh Duffy
Platform
Plate-Forme d’Immuno-monitoring
Milena Hasan
Platform
Plateforme Single Cell
Valentina Libri
Cours
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
2022-06-30 09:00:00
2022-07-06 18:00:00
Europe/Paris
Single cell gene expression and beyond
Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]
28 Rue du Docteur Roux, Paris, France
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
2018-12-02 09:00:00
2018-12-15 18:00:00
Europe/Paris
10th HKU-Pasteur Immunology Course: December 2-15, 2018
‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]
HKU-Pasteur Research Centre, Hong Kong
Projets
Projet Transversal
Outils
Financements
Publications
Télécharger-
2023In vitro cellular reprogramming to model gonad development and its disorders., Sci Adv 2023 Jan; 9(1): eabn9793.
-
2022Defective activation and regulation of type I interferon immunity is associated with increasing COVID-19 severity., Nat Commun 2022 Nov; 13(1): 7254.
-
2022IRF8 regulates efficacy of therapeutic anti-CD20 monoclonal antibodies., Eur J Immunol 2022 Aug; (): .
-
2022Putative Biomarkers of Environmental Enteric Disease Fail to Correlate in a Cross-Sectional Study in Two Study Sites in Sub-Saharan Africa., Nutrients 2022 Aug; 14(16): .
-
2022Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission., Nat Commun 2022 Jul; 13(1): 4123.
-
2022Variability of Primary Sjögren’s Syndrome Is Driven by Interferon α and Interferon α Blood Levels Are Associated With the Class II HLA-DQ Locus., Arthritis Rheumatol 2022 Jun; (): .
-
2022Dengue virus NS1 protein conveys pro-inflammatory signals by docking onto high-density lipoproteins., EMBO Rep 2022 May; (): e53600.
-
2022Targeting chalcone binding sites in living Leishmania using a reversible fluorogenic benzochalcone probe., 2022 Mars.
-
2022Integrative genetic and immune cell analysis of plasma proteins in healthy donors identifies novel associations involving primary immune deficiency genes., Genome Med 2022 03; 14(1): 28.
-
2022Identification of bipotent progenitors that give rise to myogenic and connective tissues in mouse., Elife 2022 Feb; 11(): .
-
+Voir la liste complète de publications
Contact
Phone: 01.40.61.30.27. Email: crt-tc@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France