Timeline

Sylvie Pochet

Group Leader

15 Jan 2023

publication

Synthesis and structure-activity relationship studies of original cyclic diadenosine derivatives as nanomolar inhibitors of NAD kinase from pathogenic bacteria.

29 Aug 2022

publication

Structure-based design, synthesis and biological evaluation of a NAD+ analogue targeting Pseudomonas aeruginosa NAD kinase.

30 Apr 2021

publication

Noncanonical DNA polymerization by aminoadenine-based siphoviruses.

22 Oct 2020

publication

New Chemical Probe Targeting Bacterial NAD Kinase.

13 Mar 2020

publication

From Substrate to Fragments to Inhibitor Active In Vivo against Staphylococcus aureus.

03 Jan 2020

publication

An expedient synthesis of flexible nucleosides through enzymatic glycosylation of proximal and distal fleximer bases

20 Jun 2019

publication

Crystal structure of the Trypanosoma cruzi EIF4E5 translation factor homologue in complex with mRNA cap-4

02 Jan 2019

publication

2′-Deoxyribonucleoside 5′-triphosphates bearing 4-phenyl and 4-pyrimidinyl imidazoles as DNA polymerase substrates

06 Jul 2018

publication

Enzymatic synthesis of random sequences of RNA and RNA analogues by DNA polymerase theta mutants for the generation of aptamer libraries

09 Mar 2018

project

Protein synthesis machinery of Trypanosomatid as a therapeutic target

12 Sep 2017

publication

An expedient synthesis of flexible nucleosides via a regiocontrolled enzymatic glycosylation of functionalized imidazoles

29 Nov 2016

publication

8-Thioalkyl-adenosine derivatives inhibit Listeria monocytogenes NAD kinase through a novel binding mode.

28 Apr 2016

event

2016 XXII IRT

11 Mar 2016

publication

Expedient and generic synthesis of imidazole nucleosides by enzymatic transglycosylation

07 Sep 2015

project

FP7 Targeting the Leishmania kinome for the development of novel anti-parasitic strategies (LeishDrug)

03 Sep 2015

project

Inhibitors of Pseudomonas aeruginosa IMPDH

20 Aug 2015

project

Inhibitors of TMPK from Mycobacterium tuberculosis

03 Aug 2015

patent

NOVEL ANTIBACTERIAL COMPOUNDS

03 Aug 2015

project

Inhibitors of the 2’-deoxyribonucleotide N-hydrolase DNPH1, an enzyme involved in cellular growth and angiogenesis

28 Jul 2015

project

Inhibitors of bacterial NAD kinases

29 Apr 2015

publication

Unprecedented formation of 8(R),5′-O-cycloribonucleosides through a triflation reaction of purine ribonucleosides

12 Nov 2014

publication

A convenient synthesis of 4(5)-(hetero)aryl-1H-imidazoles via microwave-assisted Suzuki-Miyaura cross-coupling reaction

01 Aug 2014

publication

6-(Hetero)Arylpurine nucleotides as inhibitors of the oncogenic target DNPH1: synthesis, structural studies and cytotoxic activities

26 Feb 2014

publication

Synthesis of novel 9-aryl and heteroarylpurine derivatives via copper mediated coupling reaction

19 Nov 2013

publication

N (6)-substituted AMPs inhibit mammalian deoxynucleotide N-hydrolase DNPH1

19 Jan 2013

publication

Structure of the oncoprotein Rcl bound to three nucleotide analogues

06 Jun 2012

publication

Screening and in situ synthesis using crystals of a NAD kinase lead to a potent antistaphylococcal compound.

01 Jan 2012

publication

Drug design and identification of potent leads against mycobacterium tuberculosis thymidine monophosphate kinase

09 Aug 2011

publication

In-plate protein crystallization, in situ ligand soaking and X-ray diffraction

20 Oct 2010

publication

Probing the active site of the deoxynucleotide N-hydrolase Rcl encoded by the rat gene c6orf108

29 Oct 2008

publication

Crystal structure of poxvirus thymidylate kinase: an unexpected dimerization has implications for antiviral therapy

16 May 2008

publication

In vivo reshaping the catalytic site of nucleoside 2′-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution

25 Apr 2008

publication

Substituted benzyl-pyrimidines targeting thymidine monophosphate kinase of Mycobacterium tuberculosis: synthesis and in vitro anti-mycobacterial activity

01 Jan 2008

publication

Expanding substrate specificity of nucleoside 2′-deoxyribosyltransferase

08 Aug 2007

publication

NAD kinases use substrate-assisted catalysis for specific recognition of NAD

10 Jul 2007

publication

Enantioselectivity of human AMP, dTMP and UMP-CMP kinases

02 Jul 2007

publication

Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives — a screening based on affinity for the UMP-CMP binding site

01 Jan 2007

publication

Looking for new pyrimidine acyclic nucleotide analogues designed for phosphorylation by human UMP-CMP kinase

01 Jan 2007

publication

A new family of inhibitors of Mycobacterium tuberculosis thymidine monophosphate kinase

01 Jan 2007

publication

Crystal structure and photochemical behavior in solution of the 3′-N-sulfamate analogue of thymidylyl(3′-5′)thymidine

27 Oct 2006

publication

Recognition of nucleotide analogs containing the 7,8-dihydro-8-oxo structure by the human MTH1 protein

01 Dec 2005

publication

Substrate specificity of vaccinia virus thymidylate kinase

01 Apr 2005

publication

Mechanism of the antiviral action of 1-beta-D-arabinofuranosylcytosine on Borna disease virus

01 Apr 2005

publication

LEA3D: a computer-aided ligand design for structure-based drug design

15 Feb 2005

publication

Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine

30 Apr 2004

publication

APOBEC3G is a single-stranded DNA cytidine deaminase and functions independently of HIV reverse transcriptase

01 Apr 2004

publication

2′-fluoro-2′-deoxycytidine inhibits Borna disease virus replication and spread

27 Feb 2004

publication

Probing the substrate recognition mechanism of the human MTH1 protein by nucleotide analogs

01 Dec 2003

publication

Replication of hexitol oligonucleotides as a prelude to the propagation of a third type of nucleic acid in vivo

15 Sep 2003

publication

Thymidine and thymidine-5′-O-monophosphate analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase

28 Aug 2003

publication

3′-C-branched-chain-substituted nucleosides and nucleotides as potent inhibitors of Mycobacterium tuberculosis thymidine monophosphate kinase

01 Aug 2003

publication

Comparative study of purine and pyrimidine nucleoside analogues acting on the thymidylate kinases of Mycobacterium tuberculosis and of humans

01 May 2003

publication

Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification

01 May 2003

publication

Design of Mycobacterium tuberculosis thymidine monophosphate kinase inhibitors

01 Mar 2003

publication

A short path synthesis of [13C/15N] multilabeled pyrimidine nucleosides starting from glucopyranose nucleosides

01 Dec 2002

publication

A parallel synthesis scheme for generating libraries of DNA polymerase substrates and inhibitors

01 Oct 2002

publication

Synthesis and evaluation of thymidine-5′-O-monophosphate analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase

01 May 2002

publication

Synthesis and recognition by DNA polymerases of a reactive nucleoside, 1-(2-deoxy-beta-D-erythro-pentofuranosyl)-imidazole-4-hydrazide

01 Jan 2002

publication

Nucleoside analogues as inhibitors of thymidylate kinases: possible therapeutic applications

01 Jan 2002

publication

Substrate recognition by the human MTH1 protein

01 Aug 2001

publication

Reverse transcriptase incorporation of 1,5-anhydrohexitol nucleotides

01 Jun 2001

publication

Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes

24 Oct 2000

publication

Enzymatic incorporation in DNA of 1,5-anhydrohexitol nucleotides

01 Jul 1999

publication

Assay of nucleoside 2-deoxyribosyltransferase activity with pyruvate kinase/lactate dehydrogenase coupling system

01 Sep 1996

publication

Ambiguous base pairing of the purine analogue 1-(2-deoxy-beta-D-ribofuranosyl)-imidazole-4-carboxamide during PCR

01 Jan 1996

publication

Construction of a self-complementary nucleoside from deoxyguanosine

15 Feb 1994

publication

Genetic characterization of polypeptide deformylase, a distinctive enzyme of eubacterial translation

25 Dec 1993

publication

Directed evolution of biosynthetic pathways. Recruitment of cysteine thioethers for constructing the cell wall of Escherichia coli

11 Dec 1990

publication

Synthesis and enzymatic polymerisation of 5-amino-1-(2′-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide-5′- triphosphate

01 Feb 1989

publication

Preparation and use of a universal primed Sepharose for the purification of DNA-binding proteins

01 Aug 1988

publication

IR and UV studies on stability and conformations of short DNA duplexes containing a no-base residue: coexistence of B and Z conformations

25 Feb 1988

publication

Solid-supported ligation primer

24 Jan 1986

publication

A little guide for advanced search:

Synthesis and structure-activity relationship studies of original cyclic diadenosine derivatives as nanomolar inhibitors of NAD kinase from pathogenic bacteria.

Structure-based design, synthesis and biological evaluation of a NAD+ analogue targeting Pseudomonas aeruginosa NAD kinase.

Noncanonical DNA polymerization by aminoadenine-based siphoviruses.

New Chemical Probe Targeting Bacterial NAD Kinase.

From Substrate to Fragments to Inhibitor Active In Vivo against Staphylococcus aureus.

An expedient synthesis of flexible nucleosides through enzymatic glycosylation of proximal and distal fleximer bases

Crystal structure of the Trypanosoma cruzi EIF4E5 translation factor homologue in complex with mRNA cap-4

2′-Deoxyribonucleoside 5′-triphosphates bearing 4-phenyl and 4-pyrimidinyl imidazoles as DNA polymerase substrates

Enzymatic synthesis of random sequences of RNA and RNA analogues by DNA polymerase theta mutants for the generation of aptamer libraries

Protein synthesis machinery of Trypanosomatid as a therapeutic target

An expedient synthesis of flexible nucleosides via a regiocontrolled enzymatic glycosylation of functionalized imidazoles

8-Thioalkyl-adenosine derivatives inhibit Listeria monocytogenes NAD kinase through a novel binding mode.

2016 XXII IRT

Expedient and generic synthesis of imidazole nucleosides by enzymatic transglycosylation

FP7 Targeting the Leishmania kinome for the development of novel anti-parasitic strategies (LeishDrug)

Inhibitors of Pseudomonas aeruginosa IMPDH

Inhibitors of TMPK from Mycobacterium tuberculosis

NOVEL ANTIBACTERIAL COMPOUNDS

Inhibitors of the 2’-deoxyribonucleotide N-hydrolase DNPH1, an enzyme involved in cellular growth and angiogenesis

Inhibitors of bacterial NAD kinases

Unprecedented formation of 8(R),5′-O-cycloribonucleosides through a triflation reaction of purine ribonucleosides

A convenient synthesis of 4(5)-(hetero)aryl-1H-imidazoles via microwave-assisted Suzuki-Miyaura cross-coupling reaction

6-(Hetero)Arylpurine nucleotides as inhibitors of the oncogenic target DNPH1: synthesis, structural studies and cytotoxic activities

Synthesis of novel 9-aryl and heteroarylpurine derivatives via copper mediated coupling reaction

N (6)-substituted AMPs inhibit mammalian deoxynucleotide N-hydrolase DNPH1

Structure of the oncoprotein Rcl bound to three nucleotide analogues

Screening and in situ synthesis using crystals of a NAD kinase lead to a potent antistaphylococcal compound.

Drug design and identification of potent leads against mycobacterium tuberculosis thymidine monophosphate kinase

In-plate protein crystallization, in situ ligand soaking and X-ray diffraction

Probing the active site of the deoxynucleotide N-hydrolase Rcl encoded by the rat gene c6orf108

Crystal structure of poxvirus thymidylate kinase: an unexpected dimerization has implications for antiviral therapy

In vivo reshaping the catalytic site of nucleoside 2′-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution

Substituted benzyl-pyrimidines targeting thymidine monophosphate kinase of Mycobacterium tuberculosis: synthesis and in vitro anti-mycobacterial activity

Expanding substrate specificity of nucleoside 2′-deoxyribosyltransferase

NAD kinases use substrate-assisted catalysis for specific recognition of NAD

Enantioselectivity of human AMP, dTMP and UMP-CMP kinases

Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives — a screening based on affinity for the UMP-CMP binding site

Looking for new pyrimidine acyclic nucleotide analogues designed for phosphorylation by human UMP-CMP kinase

A new family of inhibitors of Mycobacterium tuberculosis thymidine monophosphate kinase

Crystal structure and photochemical behavior in solution of the 3′-N-sulfamate analogue of thymidylyl(3′-5′)thymidine

Recognition of nucleotide analogs containing the 7,8-dihydro-8-oxo structure by the human MTH1 protein

Substrate specificity of vaccinia virus thymidylate kinase

Mechanism of the antiviral action of 1-beta-D-arabinofuranosylcytosine on Borna disease virus

LEA3D: a computer-aided ligand design for structure-based drug design

Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine

APOBEC3G is a single-stranded DNA cytidine deaminase and functions independently of HIV reverse transcriptase

2′-fluoro-2′-deoxycytidine inhibits Borna disease virus replication and spread

Probing the substrate recognition mechanism of the human MTH1 protein by nucleotide analogs

Replication of hexitol oligonucleotides as a prelude to the propagation of a third type of nucleic acid in vivo

Thymidine and thymidine-5′-O-monophosphate analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase

3′-C-branched-chain-substituted nucleosides and nucleotides as potent inhibitors of Mycobacterium tuberculosis thymidine monophosphate kinase

Comparative study of purine and pyrimidine nucleoside analogues acting on the thymidylate kinases of Mycobacterium tuberculosis and of humans

Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification

Design of Mycobacterium tuberculosis thymidine monophosphate kinase inhibitors

A short path synthesis of [13C/15N] multilabeled pyrimidine nucleosides starting from glucopyranose nucleosides

A parallel synthesis scheme for generating libraries of DNA polymerase substrates and inhibitors

Synthesis and evaluation of thymidine-5′-O-monophosphate analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase

Synthesis and recognition by DNA polymerases of a reactive nucleoside, 1-(2-deoxy-beta-D-erythro-pentofuranosyl)-imidazole-4-hydrazide

Nucleoside analogues as inhibitors of thymidylate kinases: possible therapeutic applications

Substrate recognition by the human MTH1 protein

Reverse transcriptase incorporation of 1,5-anhydrohexitol nucleotides

Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes

Enzymatic incorporation in DNA of 1,5-anhydrohexitol nucleotides

Assay of nucleoside 2-deoxyribosyltransferase activity with pyruvate kinase/lactate dehydrogenase coupling system

Ambiguous base pairing of the purine analogue 1-(2-deoxy-beta-D-ribofuranosyl)-imidazole-4-carboxamide during PCR

Construction of a self-complementary nucleoside from deoxyguanosine

Genetic characterization of polypeptide deformylase, a distinctive enzyme of eubacterial translation

Directed evolution of biosynthetic pathways. Recruitment of cysteine thioethers for constructing the cell wall of Escherichia coli

Synthesis and enzymatic polymerisation of 5-amino-1-(2′-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide-5′- triphosphate

Preparation and use of a universal primed Sepharose for the purification of DNA-binding proteins

IR and UV studies on stability and conformations of short DNA duplexes containing a no-base residue: coexistence of B and Z conformations

Solid-supported ligation primer

NMR, CD and IR spectroscopies of a tridecanucleotide containing a no-base residue: coexistence of B and Z conformations