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© Institut Pasteur
Cristaux de cellulase, enzyme purifiée de Clostridium thermocellum permettant la digestion de la cellulose. Image colorisée.
Publication : Nucleic acids research

NMR, CD and IR spectroscopies of a tridecanucleotide containing a no-base residue: coexistence of B and Z conformations

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 24 Jan 1986

Pochet S, Huynh-Dinh T, Neumann JM, Tran-Dinh S, Adam S, Taboury J, Taillandier E, Igolen J

Link to Pubmed [PMID] – 3945553

Nucleic Acids Res. 1986 Jan;14(2):1107-26

The synthesis of the tridecadeoxynucleotide d(CGm5CGCGxACATGT), where x is the 1-cyano-2-deoxy-beta-D-erythropentofuranose, is described. The NMR, IR, CD studies at various salt concentrations and temperatures of this oligomer show that the B and Z conformations are simultaneously present in the same short DNA fragment. A single apurinic residue is sufficient for the coexistence of the B and Z helices on this oligomer.

http://www.ncbi.nlm.nih.gov/pubmed/3945553