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© Research
Publication : Bioorganic & medicinal chemistry letters

Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine

Team: Archived: Group : Chemistry and Biocatalysis
Team: Archived: Nuclear Magnetic Resonance Of Biomolecules
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Bioorganic & medicinal chemistry letters - 15 Feb 2005

Cadena-Amaro C, Delepierre M, Pochet S

Link to Pubmed [PMID] – 15686914

Bioorg. Med. Chem. Lett. 2005 Feb;15(4):1069-73

The nucleoside 2-amino-9-(2-deoxy-beta-d-ribofuranosyl)-7,8-dihydro-8-oxo-purine (dJ) was obtained in eight steps from 2′-deoxyguanosine. The appropriate protected phosphoramidite was synthesized and incorporated into DNA oligonucleotides. The thermal stability of heteroduplexes containing 2-amino-8-oxopurine (J) was investigated by UV-thermal denaturation experiments. The results obtained can be interpreted by the base pairing schemes involving the two edges of dJ depending on the anti and syn orientations.