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© Institut Pasteur
Cristaux de cellulase, enzyme purifiée de Clostridium thermocellum permettant la digestion de la cellulose. Image colorisée.
Publication : Nucleosides, nucleotides & nucleic acids

Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification

Team: Group : Chemistry and Biocatalysis
Member: Untitled
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleosides, nucleotides & nucleic acids - 01 May 2003

Pochet S, Dugué L, Strobel H

Link to Pubmed [PMID] – 14565490

Nucleosides Nucleotides Nucleic Acids 2003 May-Aug;22(5-8):1665-7

The synthesis of 1-(2-deoxy-beta-D-erythro-pentofuranosyl)imidazole-4-hydrazide having the features of an ambigous base is reported. The recognition of the analogue by DNA polymerases as an incoming triphosphate as well as a template base was investigated. The mutagenic properties was evaluated by PCR. The potential of this new monomer for DNA diversification is illustrated by the reactivity of the nucleobase towards various aldehydes.