Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share

About

EpiChBio Team

Projects

1/ Epigenetics chemical targeting to reprogram cancer cells and human infections

DNA methylation and epigenetic modifications, in general, are reversible and can be modulated by chemical agents. It is in this context we have developed an innovative interdisciplinary approach aiming at the development of new inhibitors of DNA methylation to modulate gene expression. We have validated their use to demethylate hypermethylated gene promoters and reactivate genes in cancer cells. We are investigating the effect of this demethylation on the reprogramming of cancer cells and its consequences. In collaboration we are extending the use of the compounds to other human diseases, as parasite and bacterial infection. The rational is based on the fact that epigenetic changes confer to parasites the phenotypic plasticity, which characterise their progression through the different stages and environments of their life cycle, and bacterial pathogens and viruses alter the epigenetic modifications of the host.

 2/ Design and synthesis of novel chemical libraries for epigenetic targets

Recent genome-wide data have shown that the key actors of the epigenetic regulation are interlinked and cooperate to induce and maintain aberrant patterns in cancer cells and their microenvironment. An interesting model is thus to address both DNA and histone methylation regulation. We are thus interested in the design of novel inhibitors of HMT (histone methylatransferases). Two are the hurdles identified today: (i) find new chemical scaffolds and (ii) design selective inhibitors when targeting the co-factor pocket, since all share the same co-factor, S-adenosyl-L-methionine (AdoMet). Since the hit rate in screening campaigns against DNMTs and HMTs is particularly low (<0.01%), we are designing an original focused chemical library containing hydrophilic molecules, based on the chemical scaffolds we have developed for DNMTs, that will be tested on a large panel of DNA and histone methyltransferase.

 

 3/ Use of chemical probes to dissect the molecular Epigenetic mechanisms in diseases

The chemical tools we design and synthetize will bring an alternative approach to address the biological questions in the understanding of human diseases. For example, an important open question in cancer is what specifically hypermethylates tumor suppressor genes in cancer cells when a global hypomethylation is observed? DNMT inhibitors can be used as chemical probes to address this question. These investigations involve Chemistry and Biology steps: 1) conception and synthesis of chemical probes; 2) trapping experiments for purification of protein as well as for nucleic acid partners; 3) identification of the partners and 4) validation of the identified proteins and nucleic acids as DNMT partners.

 

Major Collaborations

2010 – 2014 Melanoma field – Dr. G. Favre, ICR, France; Dr L. Lamant, IUC, France; Dr. Luisa Lanfrancone, IEO, Italy; Dr. D. Marzese, JWCI, USA, Prof Carmen Jeronimo, ICBAS, Portugal.

2008 – 2018 Epigenetic fieldfor chemistry: Dr. D. Guianvarc’h, UPMC, France; Prof. C. Ferroud, CNAM, France; Prof. A. Mai, Università La Sapienza, Italy; Prof. G. Sbardella, Università di Salerno, Italy; Prof. A. Gagnon, UQAM, Canada; Dr. A. Kanamura, U. Oxford, UK; Prof. Ganesan, UEA, UK; – for biology: Prof. A. Jeltsch, Suttgart University, Germany; Dr. J. Tost, CEA, France; Dr L. Mourey, IPBS, France; Prof. P. Jones, Van Andel Institut, USA; Dr M. Frye, U. Cambridge, UK; Prof. B. Huntly, U. Cambridge, UK; Dr A Scherf, I. Pasteur, France.

1999 – 2010 Topoisomerase field  for chemistry: Drs. C. Monneret & D. Dauzonne, I. Curie, France; Drs. A. Garbesi & M. Capobianco, ISOF, Italy; Prof. S. Hecht, Institut, USA; Prof. L. Merlini, Università di Milano, Italy; for biology: Dr. Y. Pommier, NIH, USA; Prof. N. Osheroff, Vanderbilt University, USA; Dr. N. Dekker, Kalvi Institut, TU Delft, The Netherlands

Events

Early Career Unvestigators Meeting on Epigenetic Chemical Biology

Building: François Jacob – Room: Auditorium – Address: 28 Rue du Docteur Roux, Paris, France
2018-02-15 09:30:00 2018-02-15 17:00:00 Europe/Paris Early Career Unvestigators Meeting on Epigenetic Chemical Biology Cost Action CM1406 “Epigenetic Chemical Biology (EPICHEM) 28 Rue du Docteur Roux, Paris, France

Scientific fraud as an industry – Enrico M. Bucci Phd

Building: François Jacob – Room: Auditorium – Address: 28 Rue du Docteur Roux, Paris, France
2018-02-15 09:30:00 2018-02-15 11:00:00 Europe/Paris Scientific fraud as an industry – Enrico M. Bucci Phd Sbarro Health Research Organization, c/o Temple University – 1900 N 12th St – 19122 Philadelphia, PA – US Until recently, the existence of scientific fraud has been related to the misbehaviour of single researchers […] 28 Rue du Docteur Roux, Paris, France

Bisubstrate analogues as N-methyltransferase inhibitors and tool compounds for structural studies – Nathaniel I. Martin Phd

Building: Auditorium Centre J. Jacob – Room: Rdc 17C – Address: 28 Rue du Docteur Roux, 75015 Paris, France
2018-04-03 14:00:00 2018-04-03 19:00:00 Europe/Paris Bisubstrate analogues as N-methyltransferase inhibitors and tool compounds for structural studies – Nathaniel I. Martin Phd Nathaniel I. Martin Phd – Utrecht Institute for Pharmaceutical Sciences, Utrecht University The N-methyltransferases represent a large enzyme family that functions by specifically transferring a methyl group from the cofactor S-adenosyl-l-methionine (AdoMet) to a nitrogen […] 28 Rue du Docteur Roux, 75015 Paris, France

DNA bases beyond Watson & Crock – Pr. Thomas CARELL

Building: Auditorium Centre F. JACOB – Room: RDC 17C – Address: 28 Rue du Docteur Roux, Paris, France
2018-06-05 14:00:00 2018-06-05 17:00:00 Europe/Paris DNA bases beyond Watson & Crock – Pr. Thomas CARELL Pr. Thomas CARELL – Department für Chemie, LMU Müchen www.carellgroup.de<http://www.carellgroup.de/     28 Rue du Docteur Roux, Paris, France

Fundings

Contact

Phone:0140613291 Email: paola.arimondo@pasteur.fr Address 28 Rue du Docteur Roux 75015, Paris France