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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes

Team: Dynamics of Immune Responses
Member: Philippe Bousso
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2008

Celli S, Breart B, Bousso P

Link to Pubmed [PMID] – 18370151

Methods Mol. Biol. 2008;415:119-26

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.