Free-living bacteria commonly cope with and adapt to changing environments. We have shown that various families of antibiotics are capable, at concentrations as low as 1% of the MIC, to activate stress responses in pathogenic Gram-negative bacteria from different genera, and that such responses can lead to bacterial adaptation and emergence of resistance to antibiotics.
The goal of this project is to study the role of RNA modifications and variations in translation fidelity and efficiency in mediating the response to sub-MIC antibiotic stress. We to explore the link between sub-lethal antibiotic stress, t/rRNA modifications and modulation of translation fidelity/codon decoding, and contribution to antibiotic resistance. We are especially interested in the role of queuosine modification in tRNA (phenotypically uncharacterized in prokaryotes) in the response to sub-lethal antibiotic stress, as well as dihydrouridine and pseudouridine.
This project is in collaboration with Céline Fabret & Olivier Namy at I2BC, Saclay (https://www.i2bc.paris-saclay.fr/equipe-genomics-structure-and-translation/) and Virginie Marchand & Youri Motorine at Université de Lorraine, IMoPÄ (https://umsibslor.univ-lorraine.fr/fr/plateforme/epitranscriptomique-sequencage-epirna-seq)