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  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
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  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
12
Nov 2019
Status
Ongoing
Members
2
Structures
1

About

Successful extension of mass spectrometry-based (MS) proteomics to single cells is very challenging, mainly due to low analysis throughput and insufficient proteome coverage in single cells. To address these challenges, new pipelines have emerged that combine microfluidic nanodroplet (nanoPoTS: Zhu Y, Nat Commun, 2018) or flow cytometry (SCOPED-MS: Budnik B, Gen Biol, 2018; Specht H, BiorXiv, 2019) with recent quantitative proteomics approaches, such as Tandem Mass TagTM (TMT) isobaric labeling.

In an ongoing collaborative project with the Proteomics platform of the Institut Curie, the Institut Pasteur Proteomics Platform works on implementing recently established high-throughput methods for single cell proteomic analysis to the campus. Moreover, new pipelines developed in the framework of the “Emerging and Future Proteomics Technologies” Joined Research Activity (JRA) coordinated by the UTechS MSBio within of the EPIC-XS consortium will be transferred to the Institut Pasteur. EPIC-XS is a European infrastructure gathering 18 European leading groups in proteomics, including the MSBio Unit at the Institut Pasteur for France. The Proteomics Platform is open to collaborations with the scientific community to establish and apply innovative single-cell proteomics approaches.

In addition, ultrasensitive immuno-assay-based technologies are available at the CB UTechS to identify single-cell protein signatures of stimulated cells.

Technologies

SiMoA (Quanterix). Based on ultrasensitive, digital ELISA, the instrument enables quantification of 1-9 analytes in biological liquids or cell lysates with pM sensitivity, from low sample volumes

 

Image result for sp-x quanterixSP-X (Quanterix). Based on ultrasensitive, digital ELISA, like SiMoA, with a higher multiplexing capacity.