The project of the Unit RNA Biology of Fungal Pathogens deals with the study of the structure and plasticity of the transcriptomes of human fungal pathogens. We are mainly using the basidiomycete yeast Cryptococcus neoformans as model, but we are also considering additional pathogenic or non-pathogenic fungi for comparison. We are mainly interested in three dynamic features of the transcriptome: the introns, the UTRs, and the lncRNAs. Part of the project will use global approaches and diverse omics technologies to create an exhaustive list of all the mRNA and lncRNA molecules in the cells in order to study their differential expression in response to environmental cues. We are also using the strength of yeast genetics to decipher the mechanisms regulating the diversity of these features and the level of gene expression in response to these signals. These basic results are of course applied toward more virulence-oriented questions in studying how the transcriptome structure is regulated during infection and the consequences of these modifications for the fungus and host.
CryptoVIEW: Unraveling pathogenesis of Cryptococcosis using optical and multimodal visualizaion of enigmatic events and virulence aspects in vivo.
Cryptococcus neoformans and C. gattii are encapsulated yeasts that can cause life-threatening disease; mainly in immune-suppressed patients, but also in immune-competent individuals. Cryptococcosis affects the lung and may spread to the brain, manifesting itself […]
lncRNAs in Cryptococcus neoformans
During the last decade, advances in the sequencing technologies have revealed a large set of new eukaryotic RNA molecules. For instance, whereas 75% of the human genome was recently reported to be transcribed only […]
Intron dependant gene expression regulation in Cryptococcus neoformans
Most Cryptococccus neoformans genes are interrupted by introns, and alternative splicing occurs very often. In order to gain deeper insights into the distribution of introns in the C. neoformans genome, we have used RNA-Seq […]
INCEPTION – Institut Convergence for the study of Emergence of Pathology Through Individuals and Populations
IINCEPTION Goal The Inception’s goal is to develop a core structure to mobilize data resources, numerical sciences, and fundamental experimental biology in a range of health issues. Inception program uses Integrative Biology, Social Science […]
Broyage de petits volumes de cellules Contact : Frédérique Moyrand (firstname.lastname@example.org or tel:8436 /8311) Location : Fernbach-68-04-13
Transformation de Cryptococcus Contact : Frédérique Moyrand (email@example.com or tel:8436 /8311) Location : Fernbach-68-04-37A
2018Cryptococcus neoformans can form titan-like cells in vitro in response to multiple signals, PLoS Pathog. 2018 May;14(5):e1007007.
2018Titan cells formation in Cryptococcus neoformans is finely tuned by environmental conditions and modulated by positive and negative genetic regulators, PLoS Pathog. 2018 05;14(5):e1006982.
2018Introns in Cryptococcus, Mem. Inst. Oswaldo Cruz 2018;113(7):e170519.
2018Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus, J Fungi (Basel) 2018 Feb;4(1).
2017Environmental Triazole Induces Cross-Resistance to Clinical Drugs and Affects Morphophysiology and Virulence of Cryptococcus gattii and C. neoformans, Antimicrob. Agents Chemother. 2018 Jan;62(1).
2017Introns Protect Eukaryotic Genomes from Transcription-Associated Genetic Instability, Mol. Cell 2017 Aug;67(4):608-621.e6.
2016Intron retention-dependent gene regulation in Cryptococcus neoformans, Sci Rep 2016 Aug;6:32252.
2015Biofilm formation in Candida glabrata: What have we learnt from functional genomics approaches?, FEMS Yeast Res. 2016 Feb;16(1):fov111.
2015Role of Cln1 during melanization of Cryptococcus neoformans, Front Microbiol 2015;6:798.
2015Systematic functional profiling of transcription factor networks in Cryptococcus neoformans, Nat Commun 2015;6:6757.
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