The project of the Unit RNA Biology of Fungal Pathogens deals with the study of the structure and plasticity of the transcriptomes of human fungal pathogens. We are mainly using the basidiomycete yeast Cryptococcus neoformans as model, but we are also considering additional pathogenic or non-pathogenic fungi for comparison. We are mainly interested in three dynamic features of the transcriptome: the introns, the UTRs, and the lncRNAs. Part of the project will use global approaches and diverse omics technologies to create an exhaustive list of all the mRNA and lncRNA molecules in the cells in order to study their differential expression in response to environmental cues. We are also using the strength of yeast genetics to decipher the mechanisms regulating the diversity of these features and the level of gene expression in response to these signals. These basic results are of course applied toward more virulence-oriented questions in studying how the transcriptome structure is regulated during infection and the consequences of these modifications for the fungus and host.
CryptoVIEW: Unraveling pathogenesis of Cryptococcosis using optical and multimodal visualizaion of enigmatic events and virulence aspects in vivo.
Cryptococcus neoformans and C. gattii are encapsulated yeasts that can cause life-threatening disease; mainly in immune-suppressed patients, but also in immune-competent individuals. Cryptococcosis affects the lung and may spread to the brain, manifesting itself […]
lncRNAs in Cryptococcus neoformans
During the last decade, advances in the sequencing technologies have revealed a large set of new eukaryotic RNA molecules. For instance, whereas 75% of the human genome was recently reported to be transcribed only […]
Intron dependant gene expression regulation in Cryptococcus neoformans
Most Cryptococccus neoformans genes are interrupted by introns, and alternative splicing occurs very often. In order to gain deeper insights into the distribution of introns in the C. neoformans genome, we have used RNA-Seq […]
Emerging Infectious Diseases
Ever since its very early days, the Institut Pasteur has been committed to tackling emerging infections, and its work has left an extraordinary legacy. Many emerging infectious diseases are zoonoses, in which an animal […]
Antimicrobial resistance is dramatically increasing worldwide and is becoming one of the most urgent public health threats. There is an urgent need for actions to reverse the curve of antimicrobial resistance dissemination and to […]
INCEPTION – Institut Convergence for the study of Emergence of Pathology Through Individuals and Populations
IINCEPTION Goal The Inception’s goal is to develop a core structure to mobilize data resources, numerical sciences, and fundamental experimental biology in a range of health issues (Official website here : https://www.inception-program.fr/en). Inception program […]
Broyage de petits volumes de cellules Contact : Frédérique Moyrand (email@example.com or tel:8436 /8311) Location : Fernbach-68-04-13
Transformation de Cryptococcus Contact : Frédérique Moyrand (firstname.lastname@example.org or tel:8436 /8311) Location : Fernbach-68-04-37A
2021Host cell membrane microdomains and fungal infection., Cell Microbiol 2021 Aug; (): e13385.
2021Cryptococcus extracellular vesicles properties and their use as vaccine platforms., J Extracell Vesicles 2021 Aug; 10(10): e12129.
2021Insights in the molecular mechanisms of an azole stress adapted laboratory-generated Aspergillus fumigatus strain., Med Mycol 2021 Jul; 59(8): 763-772.
2021Structure, composition and biological properties of fungal extracellular vesicles, microLife, Volume 2, 2021, uqab009.
2021Commensalism instead of sex? Adapting mating to live in the gut., Cell Host Microbe 2021 06; 29(6): 856-858.
2021Population genomic analysis of Cryptococcus Brazilian isolates reveals an African type subclade distribution., G3 (Bethesda) 2021 Apr; (): .
2021Application of an optimized annotation pipeline to the Cryptococcus deuterogattii genome reveals dynamic primary metabolic gene clusters and genomic impact of RNAi loss., G3 (Bethesda) 2021 Feb; 11(2): .
2020Characterization of Extracellular Vesicles Produced by Aspergillus fumigatus Protoplasts., mSphere 2020 08; 5(4): .
2020Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives., Front Cell Infect Microbiol 2020 ; 10(): 346.
2020The Added Value of Longitudinal Imaging for Preclinical In Vivo Efficacy Testing of Therapeutic Compounds against Cerebral Cryptococcosis., Antimicrob. Agents Chemother. 2020 06; 64(7): .
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