1) Decipher the role of human antibodies, human antibody receptors (FcRs) and the cells expressing them (neutrophils, monocytes/macrophages, platelets, mast cells, basophils), and human complement during therapy and in the induction of pathologies (severe allergy & autoimmunity), using primarily mouse models “humanized” for these components.
2) Establish high-throughput plasma cell screening, analysis and/or sorting using droplet microfluidics technologies to understand the antibody response, repertoire and affinities, and demonstrate the pathogenic nature of antibodies in specific diseases .
3) Develop clinical studies (AUTOIMMUNI-B; NASA; WaspPenIP; ENDOPHEN) to understand how antibodies and their effector functions induce/regulate autoimmune and allergic diseases
4) Develop novel antibody-based therapeutics
Altogether, our research integrating fundamental, clinical and industry-driven approaches, aims at elucidating the role of antibodies, their receptors and the cells expressing them in major disease and therapy models and, hopefully, propose novel therapeutic solutions in antibody-based therapies.
- RECENT HIGHLIGHTS
- High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics. Gérard A, et al. Nat Biotechnol. 2020 Jun;38(6):715-721
- The anti-IgE mAb omalizumab induces adverse reactions by engaging Fcγ receptors. Balbino B, et al. J Clin Invest. 2020 Mar 2;130(3):1330-1335
- An engineered human Fc domain that behaves like a pH-toggle switch for ultra-long circulation persistence. Lee CH, et al. Nat Commun. 2019 Nov 26.
- An IgG-induced neutrophil activation pathway contributes to human drug-induced anaphylaxis. Jönsson F & de Chaisemartin L, et al. Sci Transl Med. 2019
- Evidence that neutrophils do not promote Echis carinatus venom-induced tissue destruction. Stackowicz J, et al. Nat Commun. 2018
- Platelets expressing IgG receptor FcγRIIA/CD32A determine the severity of experimental anaphylaxis. Beutier H, et al. Sci Immunol. 2018
- Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring. Eyer K, et al. Nat Biotechnol 2017
- IgG Fc domains that bind C1q but not effector Fcγ receptors delineate the importance of complement-mediated effector functions. Lee CH, et al. Nat Immunol 2017
- Neutrophil myeloperoxidase diminishes the toxic effects and mortality induced by lipopolysaccharide. Reber LL & Gillis CM, et al. J Exp Med 2017
