• 2025
  Mathilde Briday, Nicolas Carvalho, Natalia Oganesyan, Min-Ju Chang, Andrew Lees, Sébastien Brier, Alexandre Chenal, Comparative analysis of the structural dynamics of diphtheria toxin and CRM197 carrier proteins used in the development of conjugate vaccines, International Journal of Pharmaceutics, 2025, 675, pp.125535. ⟨10.1016/j.ijpharm.2025.125535⟩.
• 2025
  Fermon L, Germain-Amiot N, Oriol C, Rouillon A, Augagneur Y, Dréano S, Nicolas I, Chenal A, Bondon A, Chevance S, Pinel-Marie ML, Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin-antitoxin systems., FEBS J 2025 Mar; (): .
• 2024
  Chenal A, Minton AP, The effect of Ficoll 70 on thermally-induced and chemically-induced conformational transitions of an RTX protein is quantitatively accounted for by a unified excluded volume model., Phys Chem Chem Phys 2024 Sep; 26(37): 24461-24469.
• 2024
  Kleij L, Bruder E, Raoux-Barbot D, Lejal N, Nevers Q, Deloizy C, Da Costa B, Legrand L, Barrey E, Chenal A, Pronost S, Delmas B, Dhorne-Pollet S, Genomic characterization of equine influenza A subtype H3N8 viruses by long read sequencing and functional analyses of the PB1-F2 virulence factor of A/equine/Paris/1/2018., Vet Res 2024 Mar; 55(1): 36.
• 2024
  Abettan A, Nguyen MH, Ladant D, Monticelli L, Chenal A, CyaA translocation across eukaryotic cell membranes., Front Mol Biosci 2024 ; 11(): 1359408.
• 2023
  Sadi M, Carvalho N, Léger C, Vitorge B, Ladant D, Guijarro JI, Chenal A, B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles., Cell Rep Methods 2023 Nov; 3(11): 100624.
• 2023
  Proutière A, du Merle L, Garcia-Lopez M, Léger C, Voegele A, Chenal A, Harrington A, Tal-Gan Y, Cokelaer T, Trieu-Cuot P, Dramsi S, , Gallocin A, an Atypical Two-Peptide Bacteriocin with Intramolecular Disulfide Bonds Required for Activity., Microbiol Spectr 2023 Mar; (): e0508522.
• 2022
  Davi M, Sadi M, Pitard I, Chenal A, Ladant D, , A Robust and Sensitive Spectrophotometric Assay for the Enzymatic Activity of Bacterial Adenylate Cyclase Toxins., Toxins (Basel) 2022 Oct; 14(10): .
• 2022
  Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A, , Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium., BMC Biol 2022 08; 20(1): 176.
• 2021
  Voegele A, Sadi M, O'Brien DP, Gehan P, Raoux-Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A, , A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells., Adv Sci (Weinh) 2021 05; 8(9): 2003630.
• 2020
  Gonneaud A, Fakhir FZ, Landas E, Le Tallec E, Chartier-Garcia E, Almunia C, Chenal A, Forge V, Marquette C, , Development of Conformational Antibodies to Detect Bcl-xL’s Amyloid Aggregates in Metal-Induced Apoptotic Neuroblastoma Cells., Int J Mol Sci 2020 Oct; 21(20): .
• 2020
  Rodrigo-Unzueta A, Ghirardello M, Urresti S, Delso I, Giganti D, Anso I, Trastoy B, Comino N, Tersa M, D'Angelo C, Cifuente JO, Marina A, Liebau J, Mäler L, Chenal A, Albesa-Jové D, Merino P, Guerin ME, , Dissecting the Structural and Chemical Determinants of the “Open-to-Closed” Motion in the Mannosyltransferase PimA from Mycobacteria., Biochemistry 2020 Aug; 59(32): 2934-2945.
• 2020
  Sogues A, Martinez M, Gaday Q, Ben Assaya M, Graña M, Voegele A, VanNieuwenhze M, England P, Haouz A, Chenal A, Trépout S, Duran R, Wehenkel AM, Alzari PM, , Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum., Nat Commun 2020 Apr; 11(1): 1641.
• 2020
  O'Brien DP, Hourdel V, Chenal A, Brier S, Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins., Methods Mol Biol 2020 ; 2127(): 339-358.
• 2020
  Angely C, Ladant D, Planus E, Louis B, Filoche M, Chenal A, Isabey D, , Functional and structural consequences of epithelial cell invasion by Bordetella pertussis adenylate cyclase toxin., PLoS ONE 2020 ; 15(5): e0228606.
• 2019
  O'Brien DP, Cannella SE, Voegele A, Raoux-Barbot D, Davi M, Douché T, Matondo M, Brier S, Ladant D, Chenal A, , Post-translational acylation controls the folding and functions of the CyaA RTX toxin., FASEB J 2019 09; 33(9): 10065-10076.
• 2019
  Voegele A, Sadi M, Raoux-Barbot D, Douché T, Matondo M, Ladant D, Chenal A, , The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro., Toxins (Basel) 2019 02; 11(2): .
• 2018
  Voegele A, O'Brien DP, Subrini O, Sapay N, Cannella SE, Enguéné VYN, Hessel A, Karst J, Hourdel V, Perez ACS, Davi M, Veneziano R, Chopineau J, Vachette P, Durand D, Brier S, Ladant D, Chenal A, Translocation and calmodulin-activation of the adenylate cyclase toxin (CyaA) of Bordetella pertussis., Pathog Dis 2018 Nov; 76(8): .
• 2018
  Chenal A, Ladant D, Bioengineering of Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy, Toxins (Basel) 2018 Jul;10(7).
• 2017
  Hamdi K, Salladini E, O'Brien DP, Brier S, Chenal A, Yacoubi I, Longhi S, Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins, Sci Rep 2017 Nov;7(1):15544.
• 2017
  Voegele A, Subrini O, Sapay N, Ladant D, Chenal A, Membrane-Active Properties of an Amphitropic Peptide from the CyaA Toxin Translocation Region, Toxins (Basel) 2017 Nov;9(11).
• 2017
  Veneziano R, Rossi C, Chenal A, Brenner C, Ladant D, Chopineau J, Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution (Review), Biointerphases 2017 Sep;12(4):04E301.
• 2017
  Angely C, Nguyen NM, Andre Dias S, Planus E, Pelle G, Louis B, Filoche M, Chenal A, Ladant D, Isabey D, Exposure to Bordetella pertussis adenylate cyclase toxin affects integrin-mediated adhesion and mechanics in alveolar epithelial cells, Biol. Cell 2017 Aug;109(8):293-311.
• 2017
  Cannella SE, Ntsogo Enguéné VY, Davi M, Malosse C, Sotomayor Pérez AC, Chamot-Rooke J, Vachette P, Durand D, Ladant D, Chenal A, Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis, Sci Rep 2017 Feb;7:42065.
• 2017
  O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A, Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis., PLoS Biol 2017 Dec; 15(12): e2004486.
• 2016
  Hernández B, López-Tobar E, Sanchez-Cortes S, Coïc YM, Baron B, Chenal A, Kruglik SG, Pflüger F, Cohen R, Ghomi M, From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide, Phys Chem Chem Phys 2016 Sep;18(35):24437-50.
• 2016
  Hourdel V, Volant S, O'Brien DP, Chenal A, Chamot-Rooke J, Dillies MA, Brier S, MEMHDX: an interactive tool to expedite the statistical validation and visualization of large HDX-MS datasets., Bioinformatics 2016 Nov; 32(22): 3413-3419.
• 2016
  Rodrigo-Unzueta A, Martínez MA, Comino N, Alzari PM, Chenal A, Guerin ME, , Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria., J Biol Chem 2016 Jul; 291(27): 13955-13963.
• 2016
  Varela Chavez C, Haustant GM, Baron B, England P, Chenal A, Pauillac S, Blondel A, Popoff MR, The Tip of the Four N-Terminal α-Helices of Clostridium sordellii Lethal Toxin Contains the Interaction Site with Membrane Phosphatidylserine Facilitating Small GTPases Glucosylation, Toxins (Basel) 2016 Mar;8(4):90.
• 2015
  Eduardo Lopez Tobar, Belen Hernandez, Johanna Gomez, Alexandre Chenal, Jose Vicente Garcia-Ramos, Mahmoud Ghomi and Santiago Sanchez-Cortes, Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles., J. Phys. Chem. C. 2015.
• 2015
  O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A, Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion., Sci Rep 2015 Sep; 5(): 14223.
• 2015
  A. Chenal, A.C. Sotomayor-Perez and Daniel Ladant., Structure and function of RTX toxins., The Comprehensive Sourcebook of Bacterial Protein Toxins, Fourth Edition..
• 2015
  Babon A, Wurceldorf T, Almunia C, Pichard S, Chenal A, Buhot C, Beaumelle B, Gillet D, Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins, Toxicon 2016 Jun;116:56-62.
• 2015
  Varela Chavez C, Hoos S, Haustant GM, Chenal A, England P, Blondel A, Pauillac S, Lacy DB, Popoff MR, The catalytic domains of Clostridium sordellii lethal toxin and related large clostridial glucosylating toxins specifically recognize the negatively charged phospholipids phosphatidylserine and phosphatidic acid, Cell. Microbiol. 2015 Oct;17(10):1477-93.
• 2014
  Sotomayor-Pérez AC, Ladant D, Chenal A, Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion, Toxins (Basel) 2014 Dec;7(1):1-20.
• 2014
  Giganti D, Albesa-Jové D, Urresti S, Rodrigo-Unzueta A, Martínez MA, Comino N, Barilone N, Bellinzoni M, Chenal A, Guerin ME, Alzari PM, , Secondary structure reshuffling modulates glycosyltransferase function at the membrane., Nat Chem Biol 2015 Jan; 11(1): 16-8.
• 2014
  Karst JC, Ntsogo Enguéné VY, Cannella SE, Subrini O, Hessel A, Debard S, Ladant D, Chenal A, Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form, J. Biol. Chem. 2014 Oct;289(44):30702-16.
• 2014
  Selwa E, Davi M, Chenal A, Sotomayor-Pérez AC, Ladant D, Malliavin TE, Allosteric activation of Bordetella pertussis adenylyl cyclase by calmodulin: molecular dynamics and mutagenesis studies, J. Biol. Chem. 2014 Jul;289(30):21131-41.
• 2013
  Veneziano R, Rossi C, Chenal A, Devoisselle JM, Ladant D, Chopineau J, Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer, Proc. Natl. Acad. Sci. U.S.A. 2013 Dec;110(51):20473-8.
• 2013
  Subrini O, Sotomayor-Pérez AC, Hessel A, Spiaczka-Karst J, Selwa E, Sapay N, Veneziano R, Pansieri J, Chopineau J, Ladant D, Chenal A, Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin, J. Biol. Chem. 2013 Nov;288(45):32585-98.
• 2013
  Sotomayor-Pérez AC, Subrini O, Hessel A, Ladant D, Chenal A, Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein, J. Am. Chem. Soc. 2013 Aug;135(32):11929-34.
• 2012
  Karst JC, Barker R, Devi U, Swann MJ, Davi M, Roser SJ, Ladant D, Chenal A, Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin, J. Biol. Chem. 2012 Mar;287(12):9200-12.
• 2012
  Sotomayor-Pérez AC, Karst JC, Ladant D, Chenal A, Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements, Methods Mol. Biol. 2012;896:331-49.
• 2012
  Karst JC, Sotomayor-Pérez AC, Ladant D, Chenal A, Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods, Methods Mol. Biol. 2012;896:163-77.
• 2011
  Chenal A, Vendrely C, Vitrac H, Karst JC, Gonneaud A, Blanchet CE, Pichard S, Garcia E, Salin B, Catty P, Gillet D, Hussy N, Marquette C, Almunia C, Forge V, Amyloid fibrils formed by the programmed cell death regulator Bcl-xL, J. Mol. Biol. 2012 Jan;415(3):584-99.
• 2011
  Sotomayor-Pérez AC, Ladant D, Chenal A, Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states, J. Biol. Chem. 2011 May;286(19):16997-7004.
• 2010
  Chenal A, Karst JC, Sotomayor Pérez AC, Wozniak AK, Baron B, England P, Ladant D, Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin, Biophys. J. 2010 Dec;99(11):3744-53.
• 2010
  Sotomayor Pérez AC, Karst JC, Davi M, Guijarro JI, Ladant D, Chenal A, Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin, J. Mol. Biol. 2010 Mar;397(2):534-49.
• 2010
  Karst JC, Sotomayor Pérez AC, Guijarro JI, Raynal B, Chenal A, Ladant D, Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin, Biochemistry 2010 Jan;49(2):318-28.
• 2009
  Knapp O, Maier E, Mkaddem SB, Benz R, Bens M, Chenal A, Geny B, Vandewalle A, Popoff MR, Clostridium septicum alpha-toxin forms pores and induces rapid cell necrosis, Toxicon 2010 Jan;55(1):61-72.
• 2009
  Chenal A, Prongidi-Fix L, Perier A, Aisenbrey C, Vernier G, Lambotte S, Haertlein M, Dauvergne MT, Fragneto G, Bechinger B, Gillet D, Forge V, Ferrand M, Deciphering membrane insertion of the diphtheria toxin T domain by specular neutron reflectometry and solid-state NMR spectroscopy, J. Mol. Biol. 2009 Sep;391(5):872-83.
• 2009
  Aisenbrey C, Prongidi-Fix L, Chenal A, Gillet D, Bechinger B, Side chain resonances in static oriented proton-decoupled 15N solid-state NMR spectra of membrane proteins, J. Am. Chem. Soc. 2009 May;131(18):6340-1.
• 2009
  Bourdeau RW, Malito E, Chenal A, Bishop BL, Musch MW, Villereal ML, Chang EB, Mosser EM, Rest RF, Tang WJ, Cellular functions and X-ray structure of anthrolysin O, a cholesterol-dependent cytolysin secreted by Bacillus anthracis, J. Biol. Chem. 2009 May;284(21):14645-56.
• 2008
  Chenal A, Guijarro JI, Raynal B, Delepierre M, Ladant D, RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion, J. Biol. Chem. 2009 Jan;284(3):1781-9.
• 2008
  Galloux M, Vitrac H, Montagner C, Raffestin S, Popoff MR, Chenal A, Forge V, Gillet D, Membrane Interaction of botulinum neurotoxin A translocation (T) domain. The belt region is a regulatory loop for membrane interaction, J. Biol. Chem. 2008 Oct;283(41):27668-76.
• 2007
  Perier A, Chassaing A, Raffestin S, Pichard S, Masella M, Ménez A, Forge V, Chenal A, Gillet D, Concerted protonation of key histidines triggers membrane interaction of the diphtheria toxin T domain, J. Biol. Chem. 2007 Aug;282(33):24239-45.
• 2007
  Rohde JR, Breitkreutz A, Chenal A, Sansonetti PJ, Parsot C, Type III secretion effectors of the IpaH family are E3 ubiquitin ligases, Cell Host Microbe 2007 Mar;1(1):77-83.
• 2007
  Man P, Montagner C, Vernier G, Dublet B, Chenal A, Forest E, Forge V, Defining the interacting regions between apomyoglobin and lipid membrane by hydrogen/deuterium exchange coupled to mass spectrometry, J. Mol. Biol. 2007 Apr;368(2):464-72.
• 2007
  Montagner C, Perier A, Pichard S, Vernier G, Ménez A, Gillet D, Forge V, Chenal A, Behavior of the N-terminal helices of the diphtheria toxin T domain during the successive steps of membrane interaction, Biochemistry 2007 Feb;46(7):1878-87.
• 2007
  Vernier G, Chenal A, Vitrac H, Barumandzadhe R, Montagner C, Forge V, Interactions of apomyoglobin with membranes: mechanisms and effects on heme uptake, Protein Sci. 2007 Mar;16(3):391-400.
• 2006
  Bauche C, Chenal A, Knapp O, Bodenreider C, Benz R, Chaffotte A, Ladant D, Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin, J. Biol. Chem. 2006 Jun;281(25):16914-26.
• 2005
  Chenal A, Vernier G, Savarin P, Bushmarina NA, Gèze A, Guillain F, Gillet D, Forge V, Conformational states and thermodynamics of alpha-lactalbumin bound to membranes: a case study of the effects of pH, calcium, lipid membrane curvature and charge, J. Mol. Biol. 2005 Jun;349(4):890-905.
• 2005
  Batard T, Didierlaurent A, Chabre H, Mothes N, Bussières L, Bohle B, Couret MN, Ball T, Lemoine P, Focks Tejkl M, Chenal A, Clément G, Dupont F, Valent P, Krauth MT, André C, Valenta R, Moingeon P, Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy, Int. Arch. Allergy Immunol. 2005 Mar;136(3):239-49.
• 2004
  Buhot C, Chenal A, Sanson A, Pouvelle-Moratille S, Gelb MH, Ménez A, Gillet D, Maillère B, Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity, Protein Sci. 2004 Nov;13(11):2970-8.
• 2004
  Tavallaie M, Chenal A, Gillet D, Pereira Y, Manich M, Gibert M, Raffestin S, Popoff MR, Marvaud JC, Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies, FEBS Lett. 2004 Aug;572(1-3):299-306.
• 2004
  Mahmood Tavallaie, Alexandre Chenal, Daniel Gillet, Yannik Pereira, Maria Manich, Maryse Gibert, Stephanie Raffestin, Michel R Popoff, Jean Christophe Marvaud, Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies, FEBS Lett. 2004 Aug 13;572(1-3):299-306.
• 2003
  Nizard P, Gross DA, Babon A, Chenal A, Beaumelle B, Kosmatopoulos K, Gillet D, Anchoring cytokines to tumor cells for the preparation of anticancer vaccines without gene transfection in mice, J. Immunother. 2003 Jan-Feb;26(1):63-71.
• 2002
  Marvaud JC, Stiles BG, Chenal A, Gillet D, Gibert M, Smith LA, Popoff MR, Clostridium perfringens iota toxin. Mapping of the Ia domain involved in docking with Ib and cellular internalization, J. Biol. Chem. 2002 Nov;277(46):43659-66.
• 2002
  Chenal A, Savarin P, Nizard P, Guillain F, Gillet D, Forge V, Membrane protein insertion regulated by bringing electrostatic and hydrophobic interactions into play. A case study with the translocation domain of diphtheria toxin, J. Biol. Chem. 2002 Nov;277(45):43425-32.
• 2002
  Chenal A, Nizard P, Forge V, Pugnière M, Roy MO, Mani JC, Guillain F, Gillet D, Does fusion of domains from unrelated proteins affect their folding pathways and the structural changes involved in their function? A case study with the diphtheria toxin T domain, Protein Eng. 2002 May;15(5):383-91.
• 2001
  Nizard P, Chenal A, Beaumelle B, Fourcade A, Gillet D, Prolonged display or rapid internalization of the IgG-binding protein ZZ anchored to the surface of cells using the diphtheria toxin T domain, Protein Eng. 2001 Jun;14(6):439-46.
• 2001
  Nizard P, Gross DA, Chenal A, Beaumelle B, Kosmatopoulos K, Gillet D, [Anchoring cytokines to cancer cells using diphtheria toxin: better than immunotherapy by gene transfer?], J. Soc. Biol. 2001;195(3):229-34.