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© Sandrine Etienne-Manneville
Photo prise à l'avant (dans la protrusion) d'astrocytes primaires de rat en migration. Marquage par immunofluorescence montrant en rouge, p150 Glued, une protéine associée aux extrémités 'plus' des microtubules et en vert la tubuline des microtubules. La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaires.
Publication : Toxicon : official journal of the International Society on Toxinology

Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Toxicon : official journal of the International Society on Toxinology - 04 Aug 2015

Babon A, Wurceldorf T, Almunia C, Pichard S, Chenal A, Buhot C, Beaumelle B, Gillet D

Link to Pubmed [PMID] – 26253725

Toxicon 2016 Jun;116:56-62

We showed that bee venom phospholipase A2 can be used as a membrane-binding vector to anchor to the surface of cells a soluble protein fused to its C-terminus. ZZ, a two-domain derivative of staphylococcal protein A capable of binding constant regions of antibodies was fused to the C-terminus of the phospholipase or to a mutant devoid of enzymatic activity. The fusion proteins bound to the surface of cells and could themselves bind IgGs. Their fate depended on the cell type to which they bound. On the A431 carcinoma cell line the proteins remained exposed on the cell surface. In contrast, on human dendritic cells the proteins were internalized into early endosomes.

https://www.ncbi.nlm.nih.gov/pubmed/26253725