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Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

Early steps of infection We have observed that chlamydia invasion of a host cell triggers a transient ubiquitination at the entry sites. Deubiquitination follows very rapidly (20 min post infection). We have identified a […]

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

   NASOBACT est une recherche scientifique menée grâce à l’utilisation de données et d’échantillons biologiques collectés dans le cadre de la cohorte COSIPOP. Quel est l’objectif de NASOBACT. L’objectif est de caractériser les modifications […]

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

Désolé, cet article est seulement disponible en EN.

The transmission of tick-borne pathogens increases with the duration an infected tick is allowed to remain attached to the host. However, a recent study conducted at Pasteur Institute, demonstrated that Borrelia-infected tick nymphs were […]

This project intends to share expertise, developments, equipement and bioinformatics tools to apply Oxford Nanopore Technology sequencing to the detection, diagnostic and characterization of pathogens of public health importance. It corresponds to a collaboration […]

Understanding the molecular basis of host-pathogen interactions is critical for the discovery of new therapeutic approaches to treat infectious diseases, which in the face of increasing antibiotic resistance and emerging pathogens, is a major […]

Infectious diseases are a major public health problem, and identification of novel potential therapeutic candidates is mandatory in a context of growing antibiotic resistance. As classical infection biology approaches are nearly exhausted in their […]

In the human colon, mucus protects the underlying epithelium from pathogens. During infection, pathogens and resident microbiota compete for nutritional metabolites and therefore changes may alter the equilibrium in the colon ecosystem contributing to […]

Structural and functional studies of Chikungunya virus entry into host cells Deciphering Chikungunya virus cell biology by high-throughput / high-content screening Chikungunya virus infection of fibroblasts Chikungunya virus and the central nervous system infection  

The idea that Chlamydiae have been important in the evolutionary history of Archaeplastida (Plants) was first hinted at by the discovery that a surprisingly large number of genes in the Chlamydia genomes have closely-related […]

The asyptomatic infection by Chlamydia leads to severe complications such as infertility and ectopic pregnancy. We will attempt to understand how chlamydia infection induces either functional or organic infertility in female genital tract and […]

Like cellular reprogramming to stem cell fates, tumorigenesis is characterized by dysregulated cellular plasticity which facilitates the emergence of cancer cells, their survival and metastatic escape from the tumoral environment. While the contribution of […]

To characterize histopathological images stained by different colorations, we have developed a system to analyse color images. Images are segmented by a split and merge approach and by color quantization, to reduce color classes. We have also defined a criterion to choose […]

We are investigating a new global framework for smart acquisition methods in biological imaging whereby it is possible with mathematical tools to recover a high quality image from very fewer samples than the full […]

The group of Jost Enninga studies the escape of intracellular bacteria from phagosomes using advanced imaging techniques. The aim is to study the process of phagosomal membrane rupture that mediates bacterial release into the […]

The maintenance of cell identity and how it is subverted in disease are central questions in biology and medicine. The interplay between transcription factor and chromatin configurations plays a key role in maintaining cellular […]

Maintenance of genome integrity is crucial for bacteria to survive under stressful conditions, such as host immune defenses and antibiotics. Exposure to DNA damage might enhance cell-to-cell heterogeneity, eventually leading to states of protracted […]

With the group of Anna-Bella Failloux we established a method to embed salivary glands of mosquitos infected with Chikungunya virus to study its replicative cycle in situ (Vega-Rua et al., 2016; viruses 7: pp. […]

Endocytosis is an essential process used to internalize a wide range of molecules, and involved in many processes such as cell migration, cell division, immunity and pathogen invasion. Several endocytic pathways exist, enabling the […]

This project aims to study the pathogenesis of EV71-associated encephalitis. It combines in vitro and in vivo approaches.

One of our important discoveries was the identification of a bacterial protein translocated into the host nucleus upon infection (Pennini et al, 2010). This protein, which we called NUE (NUclear Effector), shows histone methyl […]

One of the major challenges in bacterial infectious disease relates to infections taking place in the bloodstream (septicemia). Despite availability of antibiotics these types of infections lead to extremely severe conditions such as septic […]

DMP of the project : https://doi.org/10.5281/zenodo.7074306 A common strategy of bacterial pathogens is active or passive uptake into host cells. There, they can localize within a bacterial containing vacuole (BCV) or access the host […]

An infection is defined by the deleterious consequences of the interactions between a pathogen and a host. Thus, studying the biology of infection reveals critical properties of hosts and pathogens, and is a way […]

Infections remain a major cause of mortality in humans. New infections are emerging, and resistance to antimicrobial drugs is widespread and expanding. There is thus an urgent need to develop novel therapeutic strategies based on rationally-designed drugs […]

With the group of Philippe Bastin we have a long-standing collaboration to study the structure of protein complexes (or “trains”) that are moved by anterograde and retrograde transport in the flagellum of trypanosomes. Towards […]

High-throughput sequencing has revolutionized our abilities to track and characterize novel bacterial strains as they emerge and disseminate globally. Genome-based epidemiological typing systems provide ultimate resolution for strain discrimination. Genome sequences may also be […]

We study the impact of the portal of entry of microbes in the intestine on host responses and microbial dissemination. To this end, we combine in vivo, ex vivo and in vitro systems, as well […]

 Signals generated by the engagement of the T cell antigen receptor are decoded and integrated by molecular complexes that form at the immunological synapse upon T cell receptor engagement. Assembly of these complexes, as […]

Neurolisteriosis is a common and frequently fatal complication of Listeria monocytogenes infection in human and ruminants. It has a high impact on human and animal health, but only little is known about its pathogenesis. […]

Elucidating the mechanisms underlying cell deformation and motility is a topic of major interest in cell biology, for they are strongly involved in cell development, immune responses, cancer and infectious diseases. This projects focuses on the […]

The intestine, and in particular the colon, comprising a mucus blanket, an epithelial monolayer and the lamina propria rich in immune cells, is constantly subjected to shear stress (flow) and peristaltic motion (stretching), both […]

A deeper understanding of mycobacterial individuality entails looking at this phenomenon in the fluctuating host environment. Micro-fabrication of host-mimicking platforms (either cell- or tissue-based) by standard photolithography or 3D-printing techniques could allow us to […]

We aim to analyze the impact of substrates on Entamoeba histolytica mode of migration during colonic mucosa invasion using live imaging and quantitative image analysis. E. histolytica, the etiological agent of amoebiasis, is a […]

Chlamydia trachomatis  is the most common bacterial cause of sexually transmitted infection and is particularly prevalent in the young population. C. trachomatis  is an obligate intracellular bacterium infecting primarily epithelial cells of the genital […]

This project is a part of the international Infect-Era program, AMOEBAC: Entamoeba histolytica-bacterium interaction and the role of this interaction in intestinal pathogenesis (http://www.infect-era.eu/2nd-call-2014) The aim of this investigation is to understand the induced […]

Sumoylation of proteins has emerged as a central regulatory mechanism influencing a wide range of cellular processes and is notably a key pathway facilitating cellular response to stress. Gene expression appears to be particularly […]

We study the host and bacterial factors involved in Listeria monocytogenes invasion of the central nervous system. To this end, we combine in vivo, ex vivo and in vitro systems, as well as imaging, […]

We study the host and bacterial factors involved in Listeria monocytogenes invasion of the placenta. To this end, we combine in vivo, ex vivo and in vitro systems, as well as imaging, cell biology, […]

The LISTERIAGEN project aims to identify host genetic factors associated with susceptibility to listeriosis.

Mechanisms of GPI-anchored protein apical sorting and plasma membrane organization in polarized epithelial cells. Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are membrane proteins with a range of conserved functions (e.g. enzymes, receptors, adhesion molecules, and surface […]

The use of new technological approaches including microfluidics and organ-on-chip strategies has permitted the design of advanced in vitro assays to address a variety of questions including in the study of human infections.  These […]

We use intestinal organoids, live imaging and molecular/cell biology approaches to study Listeria monocytogenes translocation across the intestinal epithelium.

Hepatocellular carcinoma is the principal form of primary liver cancer, the fifth most common human cancer (with 800000 new cases every year) and the 2nd cause of mortality by cancer worldwide. Hepatocellular carcinoma affects […]

Fluorescent live cell imaging and single-cell analysis provide a clear glimpse into bacterial individuality. Discrete subpopulations can be separated either by conventional FACS or droplet sorting, and enriched subsets can be subjected to global […]

Cette page est destinée aux participants à l’étude MONALISA, afin d’y retrouver : les informations relatives à la recherche en cours la synthèse des résultats des études auxquelles ils ont participé dernière actualisation : […]

We have developed a method to perform the detection and the tracking of microscopic spots directly on four dimensional (3D+t) image data. It extends our previous work by being able to detect with high […]

What’s best to study humans and their infectious deseases than Human emulation system ? It’s now possible at Pasteur to develop real Organs-on-chips with cells from different sources, patient derived or cell line. At […]

Prion-like proteins spreading in neurodegenerative diseases (NDs): role of Tunneling Nanotubes (TNTs) NDs are protein conformational disorders linked to the propagation of protein misfolding in the brain in a prion-like manner. A seminal discovery […]

Mechanical forces trigger many cellular functions and change the geometry and physical properties of a tissue. Very few studies look at the biomechanisms of invasion of microbes at tissue level. – In this project […]

Our proposal aims to decipher novel subversion strategies used by Leishmania (i) to withstand, circumvent or inhibit anti-microbial activities of host macrophages and (ii) to interfere with the induction of Leishmania-specific immune responses. We […]

Role of TNTs in tumor networking, heterogeneity, and resistance to therapy. In cancers such as glioblastoma (GMB) and neuroblastoma (NB), intra-tumoral heterogeneity plays a major role in tumor proliferation, trans-differentiation and invasion, as well […]

One of the significant drivers of phenotypic heterogeneity is noise in gene expression, which in turn is greatly influenced by fluctuations in the number of RNA molecules. Subcellular visualization of RNA turnover and quality […]

Artificial Intelligence theory is intrinsically linked to our understanding of how a brain computes. Indeed, artificial neural networks, that have shown their capabilities in addressing a large class of classification and prediction problems, mimic […]

To understand protein functions and molecular network organisation, it is important to determine the localisation of proteins at subcellular level. The in cellulo probing of molecular assemblies is usually performed with microscopy techniques which […]

Listeriosis is a leading cause of death from food-transmitted bacterial pathogens. It can cause infections in the blood stream and brain, and lead to abortion, neonatal infection and fetal death. The causative organism, Listeria […]

An important research axis of the laboratory is to modify nucleic acids with chemical groups in order to improve their therapeutic usefulness. Particularly, modified aptamers (i.e. oligonucleotides capable of binding to targets with high […]

The Think tank Mucosal Immunology gathers several research units form the departments of immunology, microbiology, cellular biology and epidemilogy and infection, as well as from the Institut Imagine (Necker) and Hôpital Paul Brousse. It […]

Unraveling the structure/function of TNTs in vitro and in vivo. While TNTs may be involved in cell-to-cell communication in many physiological processes as well as diseases, their physiological relevance is unclear as their existence […]

We are interested in deciphering the relationships that link gut microbiota with the immune system and the brain. So far, the mechanisms underlying these interplay, and most importantly their consequences, remain largely unknown. Therefore […]