Infectious diseases are a major public health problem, and identification of novel potential
therapeutic candidates is mandatory in a context of growing antibiotic resistance. As classical infection biology approaches are nearly exhausted in their current capacity to address the emergence of multi-drug resistance, new conceptual approaches are required. Targeting common cellular pathways hijacked by pathogens during their encounter with host cells represents a novel strategy to inhibit infection. The implementation of comprehensive system biology approaches, together with robust assays from front edge technologies, would be the most efficient method for identifying critical cellular hubs to be targeted as novel therapeutic candidates. The objective of our proposal is to identify such cellular hubs by obtaining unprecedented details of the infection strategies of major model bacterial pathogen.