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© Research
Publication : Nature communications

The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature communications - 22 Apr 2020

Addi C, Presle A, Frémont S, Cuvelier F, Rocancourt M, Milin F, Schmutz S, Chamot-Rooke J, Douché T, Duchateau M, Giai Gianetto Q, Salles A, Ménager H, Matondo M, Zimmermann P, Gupta-Rossi N, Echard A,

Link to Pubmed [PMID] – 32321914

Link to DOI [DOI] – 10.1038/s41467-020-15205-z

Nat Commun 2020 Apr; 11(1): 1941

Cytokinesis requires the constriction of ESCRT-III filaments on the side of the midbody, where abscission occurs. After ESCRT recruitment at the midbody, it is not known how the ESCRT-III machinery localizes to the abscission site. To reveal actors involved in abscission, we obtained the proteome of intact, post-abscission midbodies (Flemmingsome) and identified 489 proteins enriched in this organelle. Among these proteins, we further characterized a plasma membrane-to-ESCRT module composed of the transmembrane proteoglycan syndecan-4, ALIX and syntenin, a protein that bridges ESCRT-III/ALIX to syndecans. The three proteins are highly recruited first at the midbody then at the abscission site, and their depletion delays abscission. Mechanistically, direct interactions between ALIX, syntenin and syndecan-4 are essential for proper enrichment of the ESCRT-III machinery at the abscission site, but not at the midbody. We propose that the ESCRT-III machinery must be physically coupled to a membrane protein at the cytokinetic abscission site for efficient scission, uncovering common requirements in cytokinesis, exosome formation and HIV budding.

https://pubmed.ncbi.nlm.nih.gov/32321914