David is a young investigator at the Institut Pasteur in the department of Microbiology where he started his group in 2014. David obtained a engineer degree from AgroParisTech as well as a master degree and PhD from Paris Diderot University for his work performed at the Institut Pasteur on the integron bacterial recombination system. He then joined the laboratory of Luciano Marraffini at the Rockefeller University as a postdoctoral fellow where he started to work on CRISPR systems. David is interested in applying engineering principles to better understand and fight pathogenic bacteria.
“Evolutionary ecology of CRISPR-Cas” by Edze WESTRA – MICROBIOLOGY DEPARTEMENTAL SEMINAR
Prophage elements as a significant source of genetic diversity among epidemic C. difficile 027 strains
The capacity to rapidly and accurately identify virulent strains of C. difficile in order to prevent severe and fatal infections remains a major chal- lenge for clinicians and investigators. Also, the evolution of an […]
Studying and fighting pathogenic bacteria with the help of CRISPR
CRISPR loci and the associated Cas genes are the adaptive immune system of archeae and bacteria. They have the ability to capture pieces of DNA from viruses and other invading genetic elements , and […]
PhageTerm: Determining Bacteriophage Termini and Packaging using NGS data.
Bacteriophages are the most abundant viruses on earth and display an impressive genetic as well as morphologic diversity. Among those, the most common order of phages is the Caudovirales, whose viral particles packages linear […]
2017Inhibition of NHEJ repair by type II-A CRISPR-Cas systems, https://doi.org/10.1101/170647.
2017Engineered CRISPR-Cas9 system enables noiseless, fine-tuned and multiplexed repression of bacterial genes, bioRxiv, July, 164384. doi:10.1101/164384..
2017Differences in Integron Cassette Excision Dynamics Shape a Trade-Off between Evolvability and Genetic Capacitance., MBio. 2017 Mar 28;8(2). pii: e02296-16. doi: 10.1128/mBio.02296-16..
2017PhageTerm: a Fast and User-friendly Software to Determine Bacteriophage Termini and Packaging Mode using randomly fragmented NGS data, Garneau JR, Depardieu F, Fortier LC, Bikard D, Monot M. 2017. PhageTerm: a Fast and User-friendly Software to Determine Bacteriophage Termini and Packaging Mode using randomly fragmented NGS data. bioRxiv doi: 10.1101/108100.
2017Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response, Molecular Cell 65 (1), 168-175.
2016A Eukaryotic-like Serine/Threonine Kinase Protects Staphylococci against Phages, Cell Host Microbe 2016 Sep;.
2016Consequences of Cas9 cleavage in the chromosome of Escherichia coli, Nucleic Acids Res. 2016 Apr;.
2015Adapting to new threats: the generation of memory by CRISPR-Cas immune systems, Mol. Microbiol. 2014 Jul;93(1):1-9.
2015Cas9 specifies functional viral targets during CRISPR-Cas adaptation, Nature 2015 Mar;519(7542):199-202.
2014Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials, Nat. Biotechnol. 2014 Nov;32(11):1146-50.
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