David is a young investigator at the Institut Pasteur in the department of Microbiology where he started his group in 2014. David obtained a engineer degree from AgroParisTech as well as a master degree and PhD from Paris Diderot University for his work performed at the Institut Pasteur on the integron bacterial recombination system. He then joined the laboratory of Luciano Marraffini at the Rockefeller University as a postdoctoral fellow where he started to work on CRISPR systems. David is interested in applying engineering principles to better understand and fight pathogenic bacteria.
ERC starting grant – CRISPAIR: Study of the interplay between CRISPR interference and DNA repair pathways towards the development of novel CRISPR tools
CRISPR-Cas loci are the adaptive immune system of archaea and bacteria. They can capture pieces of invading DNA and use this information to degrade target DNA through the action of RNA-guided nucleases. The consequences […]
Prophage elements as a significant source of genetic diversity among epidemic C. difficile 027 strains
The capacity to rapidly and accurately identify virulent strains of C. difficile in order to prevent severe and fatal infections remains a major chal- lenge for clinicians and investigators. Also, the evolution of an […]
Studying and fighting pathogenic bacteria with the help of CRISPR
CRISPR loci and the associated Cas genes are the adaptive immune system of archeae and bacteria. They have the ability to capture pieces of DNA from viruses and other invading genetic elements , and […]
PhageTerm: Determining Bacteriophage Termini and Packaging using NGS data.
Bacteriophages are the most abundant viruses on earth and display an impressive genetic as well as morphologic diversity. Among those, the most common order of phages is the Caudovirales, whose viral particles packages linear […]
2017PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data, Sci Rep 2017 Aug;7(1):8292.
2017Inhibition of NHEJ repair by type II-A CRISPR-Cas systems, https://doi.org/10.1101/170647.
2017Engineered CRISPR-Cas9 system enables noiseless, fine-tuned and multiplexed repression of bacterial genes, bioRxiv, July, 164384. doi:10.1101/164384..
2017Using CRISPR-Cas systems as antimicrobials, Current Opinion in Microbiology 2017, 37:155–160.
2017Differences in Integron Cassette Excision Dynamics Shape a Trade-Off between Evolvability and Genetic Capacitance, MBio 2017 Mar;8(2).
2017PhageTerm: a Fast and User-friendly Software to Determine Bacteriophage Termini and Packaging Mode using randomly fragmented NGS data, Garneau JR, Depardieu F, Fortier LC, Bikard D, Monot M. 2017. PhageTerm: a Fast and User-friendly Software to Determine Bacteriophage Termini and Packaging Mode using randomly fragmented NGS data. bioRxiv doi: 10.1101/108100.
2017Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response, Molecular Cell 65 (1), 168-175.
2016A Eukaryotic-like Serine/Threonine Kinase Protects Staphylococci against Phages, Cell Host Microbe 2016 Sep;.
2016Consequences of Cas9 cleavage in the chromosome of Escherichia coli, Nucleic Acids Res. 2016 Apr;.
2015Adapting to new threats: the generation of memory by CRISPR-Cas immune systems, Mol. Microbiol. 2014 Jul;93(1):1-9.
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