David is a young investigator at the Institut Pasteur in the department of Microbiology where he started his group in 2014. David obtained a engineer degree from AgroParisTech as well as a master degree and PhD from Paris Diderot University for his work performed at the Institut Pasteur on the integron bacterial recombination system. He then joined the laboratory of Luciano Marraffini at the Rockefeller University as a postdoctoral fellow where he started to work on CRISPR systems. David is interested in applying engineering principles to better understand and fight pathogenic bacteria.
LabEx IBEID – Integrative Biology of Emerging Infectious Diseases
Presentation The aim of the Integrative Biology of Emerging Infectious Diseases (IBEID) project, coordinated by Professors Philippe Sansonetti and Pascale Cossart, is to develop a structure to anticipate and tackle emerging infectious diseases (EIDs). […]
ERC CoG: In situ genetic perturbation of gut bacteria with engineered phage vectors and CRISPR
Humans live in a symbiotic relationship with trillions of microorganisms that inhabit our bodies and play an important role in health and disease. There is a growing interest in manipulating the microbiome to improve […]
Impact of chromosomal integrons on bacterial life and evolution (Chromintevol)
Bacteria exchange and recombine DNA to accelerate their evolution and adapt to new stress. Their evolvability is illustrated by the rapid emergence of multi resistant bacteria after the introduction of antibiotics. The most powerful […]
ERC starting grant – CRISPAIR: Study of the interplay between CRISPR interference and DNA repair pathways towards the development of novel CRISPR tools
CRISPR-Cas loci are the adaptive immune system of archaea and bacteria. They can capture pieces of invading DNA and use this information to degrade target DNA through the action of RNA-guided nucleases. The consequences […]
Prophage elements as a significant source of genetic diversity among epidemic C. difficile 027 strains
The capacity to rapidly and accurately identify virulent strains of C. difficile in order to prevent severe and fatal infections remains a major chal- lenge for clinicians and investigators. Also, the evolution of an […]
Studying and fighting pathogenic bacteria with the help of CRISPR
CRISPR loci and the associated Cas genes are the adaptive immune system of archeae and bacteria. They have the ability to capture pieces of DNA from viruses and other invading genetic elements , and […]
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2022Phages and their satellites encode hotspots of antiviral systems., Cell Host Microbe 2022 Mar; (): .
2021Improving sequence-based modeling of protein families using secondary structure quality assessment., Bioinformatics 2021 Jun; (): .
2021Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane, promoting fitness upon envelope stress., Elife 2021 Apr; 10(): .
2021The impact of genetic diversity on gene essentiality within the Escherichia coli species., Nat Microbiol 2021 03; 6(3): 301-312.
2021Generating functional protein variants with variational autoencoders., PLoS Comput Biol 2021 Feb; 17(2): e1008736.
2020Structure-specific DNA recombination sites: Design, validation, and machine learning-based refinement., Sci Adv 2020 Jul; 6(30): eaay2922.
2020On-target activity predictions enable improved CRISPR-dCas9 screens in bacteria, Nucleic Acids Res. 2020 Apr;.
2020Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements, Nucleic Acids Res. 2020 Jan;48(2):748-760.
2020Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects, eLife 2020;9:e51998.
2019Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels, Methods 2019 Aug;.
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