David is a young investigator at the Institut Pasteur in the department of Microbiology where he started his group in 2014. David obtained a engineer degree from AgroParisTech as well as a master degree and PhD from Paris Diderot University for his work performed at the Institut Pasteur on the integron bacterial recombination system. He then joined the laboratory of Luciano Marraffini at the Rockefeller University as a postdoctoral fellow where he started to work on CRISPR systems. David is interested in applying engineering principles to better understand and fight pathogenic bacteria.
LabEx IBEID – Integrative Biology of Emerging Infectious Diseases
Presentation The aim of the Integrative Biology of Emerging Infectious Diseases (IBEID) project, coordinated by Professors Philippe Sansonetti and Pascale Cossart, is to develop a structure to anticipate and tackle emerging infectious diseases (EIDs). […]
ERC starting grant – CRISPAIR: Study of the interplay between CRISPR interference and DNA repair pathways towards the development of novel CRISPR tools
CRISPR-Cas loci are the adaptive immune system of archaea and bacteria. They can capture pieces of invading DNA and use this information to degrade target DNA through the action of RNA-guided nucleases. The consequences […]
Prophage elements as a significant source of genetic diversity among epidemic C. difficile 027 strains
The capacity to rapidly and accurately identify virulent strains of C. difficile in order to prevent severe and fatal infections remains a major chal- lenge for clinicians and investigators. Also, the evolution of an […]
Studying and fighting pathogenic bacteria with the help of CRISPR
CRISPR loci and the associated Cas genes are the adaptive immune system of archeae and bacteria. They have the ability to capture pieces of DNA from viruses and other invading genetic elements , and […]
2020Structure-specific DNA recombination sites: Design, validation, and machine learning-based refinement., Sci Adv 2020 Jul; 6(30): eaay2922.
2020On-target activity predictions enable improved CRISPR-dCas9 screens in bacteria, Nucleic Acids Res. 2020 Apr;.
2020Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements, Nucleic Acids Res. 2020 Jan;48(2):748-760.
2020Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects, eLife 2020;9:e51998.
2019Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels, Methods 2019 Aug;.
2019Editing the microbiome the CRISPR way, Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2019 May;374(1772):20180103.
2019A matter of background: DNA repair pathways as a possible cause for the sparse distribution of CRISPR-Cas systems in bacteria, Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2019 May;374(1772):20180088.
2018Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors, PLOS Genetics 14(11): e1007749..
2018A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9, Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5.
2018Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial genes, Molecular Systems Biology (2018) 14, e7899.
+View full list of publications