David is a young investigator at the Institut Pasteur in the department of Microbiology where he started his group in 2014. David obtained a engineer degree from AgroParisTech as well as a master degree and PhD from Paris Diderot University for his work performed at the Institut Pasteur on the integron bacterial recombination system. He then joined the laboratory of Luciano Marraffini at the Rockefeller University as a postdoctoral fellow where he started to work on CRISPR systems. David is interested in applying engineering principles to better understand and fight pathogenic bacteria.
LabEx IBEID – Integrative Biology of Emerging Infectious Diseases
Presentation The aim of the Integrative Biology of Emerging Infectious Diseases (IBEID) project, coordinated by Professors Philippe Sansonetti and Pascale Cossart, is to develop a structure to anticipate and tackle emerging infectious diseases (EIDs). […]
ERC starting grant – CRISPAIR: Study of the interplay between CRISPR interference and DNA repair pathways towards the development of novel CRISPR tools
CRISPR-Cas loci are the adaptive immune system of archaea and bacteria. They can capture pieces of invading DNA and use this information to degrade target DNA through the action of RNA-guided nucleases. The consequences […]
Prophage elements as a significant source of genetic diversity among epidemic C. difficile 027 strains
The capacity to rapidly and accurately identify virulent strains of C. difficile in order to prevent severe and fatal infections remains a major chal- lenge for clinicians and investigators. Also, the evolution of an […]
Studying and fighting pathogenic bacteria with the help of CRISPR
CRISPR loci and the associated Cas genes are the adaptive immune system of archeae and bacteria. They have the ability to capture pieces of DNA from viruses and other invading genetic elements , and […]
2019Editing the microbiome the CRISPR way, Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2019 May;374(1772):20180103.
2019A matter of background: DNA repair pathways as a possible cause for the sparse distribution of CRISPR-Cas systems in bacteria, Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2019 May;374(1772):20180088.
2018Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors, Rousset F, Cui L, Siouve E, Becavin C, Depardieu F, et al. (2018) Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors. PLOS Genetics 14(11): e1007749..
2018A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9, Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5.
2018Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial genes, Molecular Systems Biology (2018) 14, e7899.
2017Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria, Nature Communications 8, 2094..
2017PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data, Sci Rep 2017 Aug;7(1):8292.
2017Using CRISPR-Cas systems as antimicrobials, Current Opinion in Microbiology 2017, 37:155–160.
2017Differences in Integron Cassette Excision Dynamics Shape a Trade-Off between Evolvability and Genetic Capacitance, MBio 2017 Mar;8(2).
2017Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response, Molecular Cell 65 (1), 168-175.
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