Plateforme de Cytométrie

Need training? Someone to look over your data? Panel design? Teaching? Plan your experiment? Got a new application or developing a new technique and you’d like to set up a collaboration with us?

Contact us (pfc@pasteur.fr) to discuss all aspects of flow cytometry, from feasibility to execution, instrument training and theory teaching and advice with sample preparation. We are available for Departmental seminars, advanced user training  and other outreach activities (secondary and undergraduate teaching). Engage the experts and ensure that your flow cytometry experimentation is done right, is reproducible and publishable.

Training for automomous use to the equipment

Assistance in using an cytometer

Advice and technical support

Scientific collaborations

Access to different data analysis software

Protocols and Cytometry Tutorials

Cytometry Glossary

Resources

Twice a week, our morning routine at the flow cytometry core facility starts roughly, at 9:30, and we basically have 2 hours to set up all the instruments, do the fluidics cleaning and quality control checks, so that we make sure that the instruments are performing well for the researchers that typically arrive around 11:30 and start their experiments.

Cell sorting

Cytometry staff are highly experienced in multicolor immune cell sorting of human and mouse cells, bacteria, virus, and others sub cellular particules. We provide upport for for RNA Seq applications (sorting in bulk or in P384).
We propose cell sorting instrumentation in BSL1, BSL2 and BSL2+ environments (see individual equipment description)

Cell Analysers

The Cytometry platform offer a range of conventional and spectral cytometers catering to the multitude of applications demanded by our researchers. We instrumentation in BSL1, BSL2 and BSL2+ environments (see individual equipment description)

• Conventional Flow Cytometry
  Today, instruments are available designed to detect up to 50 different bio‐markers on an individual cell. Typically these markers are fluorescently tagged antibodies, molecular sensors, as well as genetically encoded reporters. Our high parameters flow cytometers are a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations.
• Spectral Flow cytometry
  Since 2016, we have developed our expertise in spectral cytometry.
  For spectral flow cytometry, the “one detector, one marker” paradigm is changed.
  Emerging technology that revolutionizes the cytometry by identifying specific fluorochrome “signatures”and by eliminating need for compensation. These instruments employs a prism array to disperse the collected light over a 32 channel PMT. The measured spectra from single cells are subsequently unmixed by using reference spectra of all used dyes and the autofluorescence spectrum. Least Square Fitting algorithms are used to calculate the most accurate fit for all reference spectra, leading to an accurate determination of which dyes are present on each cell and at which intensity. These capabilities allow the detection of really close emission spectra dyes to achieve greater flexibility for panel design and more accurate visualization of results. The sensitivity is increased by removal of auto-fluorescence.

We offer you various tools to optimize your cytometry experiments :

• cytometry memory aid poster
• different cytometry protocols (antibody titration, apoptosis, cell cycle, isolation of PBMCs, calcium analysis, membrane permeability, etc.)