Since September 2005: platform of recombinant proteins (group J.Bellalou) Production of recombinant proteins in microfermentors battery (BIOPOD F800). Optimization of protein production protocols in E. coli. Protein expression analysis: electrophoresis SDS-PAGE gels, Western-blot. Protein purifications on AKTA pure and AKTA Express systems. 2002 – 2005 viral immunopathology unit at Pasteur Institute Research project on HIV. PTR in collaboration with AVENTIS-PASTEUR Company. Cell cultures (dendritic cells) . Elisa/Elispot, use of a flow cytometer (FACS) bioassay 1994 – 2002 Collection of Bacteria of the Pasteur Institute Lyophilization of bacterial strains. Production of spores suspensions Studies of the biochemical identification characters of bacterial strains Electrophoresis and chromatography techniques Participation at the quality assurance system ISO Safety and hygiene correspondent at the PFPR.
Implementation of automated procedures for high-throughput screening cultures and analytical purification of protein
The availability of recombinant proteins remains a bottleneck for many scientific projects. E.coli is the most widely expression system used, but the production of soluble proteins requires the simultaneous assessment and combination of multiple […]
Development of an immunoassay for the diagnosis of porcine cysticercosis
Neurocysticercosis (NCC) caused by Taenia solium, is one of the most important parasitic infections of the central nervous system. Both human and pigs can act as intermediate hosts carrying the larvae stage, i.e. developing […]
Rod-cone protein interactions and therapeutic perspective of retinal degenerations.
Loss of cone photoreceptors in retinitis pigmentosa (RP) leads to complete blindness. The group of Thierry Leveillard at the “Institut de la Vision” showed that injection of the RdCVF protein is a promising therapy […]
Molecular mechanisms of action of sigmaS in bacterial fitness and adaptation to the environment.
In Escherichia coli and Salmonella enterica serovar Typhimurium, the regulatory protein Crl regulates gene expression by increasing the performance of RpoS/σS sigma factor to modify global gene transcription in stationary phase and under stress […]