Signalling and Molecular interactions

About

We study the role of the protein-protein interaction domains called PDZ. PDZ domains are scaffold modules regulating the cellular trafficking, the auto-association and the catalytic activity of essential cellular proteins. We have two lines of research: the rabies virus neuronal infection and the mutations in PDZ Binding Motif (PBM)-containing proteins associated to the Usher syndrome.

Members

Nicolas Wolff

Group Leader

Head

Célia Caillet-Saguy

Permanent Researcher

Mariano Genera

PhD Student

Yanlei Zhu

PhD Student

Emilie (de La Llave) Giraud

Post-doc

Baptiste COLCOMBET-CAZENAVE

PhD Student

Tiziana Lo Campo

Undergraduate Student

Former Members

Florence Cordier

Virginie Girault

Florent Delhommel

Damien Samson

Juan Ramon Ramirez Ramos

Alain Chaffotte

Projects

project

Ongoing

4 Members

Mutations in PDZ Binding Motif (PBM)-containing proteins associated to the Usher syndrome

Nicolas Wolff (PI)

Mutations in PDZ Binding Motif (PBM)-containing proteins are also associated to the Usher syndrome, a genetic disease resulting in both hearing loss and visual impairment. Most Usher proteins are present in auditory hair cells […]

project

Ongoing

4 Members

Rabies virus neuronal infection

Nicolas Wolff (PI)

We have shown that proteins from Rabies virus during infection disrupts the interactions of the kinase MAST2 and phosphatase PTPN4 (PDZ domain) with their respective PDZ Binding Motif (PBM)-containing cellular ligands, the phosphatase PTEN […]

Fundings

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Publications

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• 2019
  Genera M, Samson D, Raynal B, Haouz A, Baron B, Simenel C, Guerois R, Wolff N, Caillet-Saguy C, Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein, Sci Rep 2019 May;9(1):7438.
• 2019
  Giraud E, Rouault E, Fiette L, Colle JH, Smirlis D, Melanitou E, Osteopontin in the host response to Leishmania amazonensis, BMC Microbiol. 2019 Feb;19(1):32.
• 2017
  Delhommel F, Cordier F, Bardiaux B, Bouvier G, Colcombet-Cazenave B, Brier S, Raynal B, Nouaille S, Bahloul A, Chamot-Rooke J, Nilges M, Petit C, Wolff N, Structural Characterization of Whirlin Reveals an Unexpected and Dynamic Supramodule Conformation of Its PDZ Tandem, Structure 2017 11;25(11):1645-1656.e5.
• 2017
  Caillet-Saguy C, Toto A, Guerois R, Maisonneuve P, di Silvio E, Sawyer K, Gianni S, Wolff N, Regulation of the Human Phosphatase PTPN4 by the inter-domain linker connecting the PDZ and the phosphatase domains, Sci Rep 2017 Aug;7(1):7875.
• 2017
  Bahloul A, Pepermans E, Raynal B, Wolff N, Cordier F, England P, Nouaille S, Baron B, El-Amraoui A, Hardelin JP, Durand D, Petit C, Conformational switch of harmonin, a submembrane scaffold protein of the hair cell mechanoelectrical transduction machinery, FEBS Lett. 2017 08;591(15):2299-2310.
• 2016
  Delhommel F, Wolff N, Cordier F, (1)H, (13)C and (15)N backbone resonance assignments and dynamic properties of the PDZ tandem of Whirlin, Biomol NMR Assign 2016 Oct;10(2):361-5.
• 2016
  Maisonneuve P, Caillet-Saguy C, Vaney MC, Bibi-Zainab E, Sawyer K, Raynal B, Haouz A, Delepierre M, Lafon M, Cordier F, Wolff N, Molecular Basis of the Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-activated Protein Kinase p38γ, J. Biol. Chem. 2016 08;291(32):16699-708.
• 2015
  Vincentelli R, Luck K, Poirson J, Polanowska J, Abdat J, Blémont M, Turchetto J, Iv F, Ricquier K, Straub ML, Forster A, Cassonnet P, Borg JP, Jacob Y, Masson M, Nominé Y, Reboul J, Wolff N, Charbonnier S, Travé G, Quantifying domain-ligand affinities and specificities by high-throughput holdup assay, Nat. Methods 2015 Aug;12(8):787-93.
• 2015
  Delhommel F, Chaffotte A, Terrien E, Raynal B, Buc H, Delepierre M, Cordier F, Wolff N, Deciphering the unconventional peptide binding to the PDZ domain of MAST2, Biochem. J. 2015 Jul;469(1):159-68.
• 2015
  Caillet-Saguy C, Maisonneuve P, Delhommel F, Terrien E, Babault N, Lafon M, Cordier F, Wolff N, Strategies to interfere with PDZ-mediated interactions in neurons: What we can learn from the rabies virus, Prog. Biophys. Mol. Biol. 2015 Oct;119(1):53-9.
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Contact

Phone:+33 1 45 68 88 72
Email:nicolas.wolff@pasteur.fr

Address
25-28 Rue du Docteur Roux
75015, Paris
France

Published on: 16 Jul 2015 • Modified on: 07 Sep 2018