Signalling and Molecular interactions

About

We study the role of the protein-protein interaction domains called PDZ. PDZ domains are scaffold modules regulating the cellular trafficking, the auto-association and the catalytic activity of essential cellular proteins. We have two lines of research: the rabies virus neuronal infection and the mutations in PDZ Binding Motif (PBM)-containing proteins associated to the Usher syndrome.

Members

Nicolas Wolff

Group Leader

Head

Célia Caillet-Saguy

Permanent Researcher

Mariano Genera

PhD Student

Yanlei Zhu

PhD Student

Baptiste COLCOMBET-CAZENAVE

PhD Student

Groups

Group

Signalling and Molecular interactions

Nicolas Wolff

Former Members

Florence Cordier

Virginie Girault

Florent Delhommel

Damien Samson

Juan Ramon Ramirez Ramos

Alain Chaffotte

Emilie (de La Llave) Giraud

Tiziana Lo Campo

Projects

project

Ongoing

5 Members

Mutations in PDZ Binding Motif (PBM)-containing proteins associated to the Usher syndrome

Nicolas Wolff (PI)

Mutations in PDZ Binding Motif (PBM)-containing proteins are also associated to the Usher syndrome, a genetic disease resulting in both hearing loss and visual impairment. Most Usher proteins are present in auditory hair cells […]

project

Ongoing

4 Members

Rabies virus neuronal infection

Nicolas Wolff (PI)

We have shown that proteins from Rabies virus during infection disrupts the interactions of the kinase MAST2 and phosphatase PTPN4 (PDZ domain) with their respective PDZ Binding Motif (PBM)-containing cellular ligands, the phosphatase PTEN […]

Fundings

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Publications

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• 2019
  Caillet J, Baron B, Boni IV, Caillet-Saguy C, Hajnsdorf E, Identification of protein-protein and ribonucleoprotein complexes containing Hfq, Sci Rep 2019 Oct;9(1):14054.
• 2019
  Khan Z, Terrien E, Delhommel F, Lefebvre-Omar C, Bohl D, Vitry S, Bernard C, Ramirez J, Chaffotte A, Ricquier K, Vincentelli R, Buc H, Prehaud C, Wolff N, Lafon M, Structure-based optimization of a PDZ-binding motif within a viral peptide stimulates neurite outgrowth, J. Biol. Chem. 2019 Sep;294(37):13755-13768.
• 2019
  Genera M, Samson D, Raynal B, Haouz A, Baron B, Simenel C, Guerois R, Wolff N, Caillet-Saguy C, Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein, Sci Rep 2019 May;9(1):7438.
• 2019
  Duhoo Y, Girault V, Turchetto J, Ramond L, Durbesson F, Fourquet P, Nominé Y, Cardoso V, Sequeira AF, Brás JLA, Fontes CMGA, Travé G, Wolff N, Vincentelli R, High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay, Methods Mol. Biol. 2019;2025:439-476.
• 2018
  Delhommel F, Cordier F, Saul F, Chataigner L, Haouz A, Wolff N, Structural plasticity of the HHD2 domain of whirlin, FEBS J. 2018 10;285(20):3738-3752.
• 2017
  Bruzzoni-Giovanelli H, Alezra V, Wolff N, Dong CZ, Tuffery P, Rebollo A, Interfering peptides targeting protein-protein interactions: the next generation of drugs?, Drug Discov. Today 2018 Feb;23(2):272-285.
• 2017
  Delhommel F, Cordier F, Bardiaux B, Bouvier G, Colcombet-Cazenave B, Brier S, Raynal B, Nouaille S, Bahloul A, Chamot-Rooke J, Nilges M, Petit C, Wolff N, Structural Characterization of Whirlin Reveals an Unexpected and Dynamic Supramodule Conformation of Its PDZ Tandem, Structure 2017 11;25(11):1645-1656.e5.
• 2017
  Caillet-Saguy C, Toto A, Guerois R, Maisonneuve P, di Silvio E, Sawyer K, Gianni S, Wolff N, Regulation of the Human Phosphatase PTPN4 by the inter-domain linker connecting the PDZ and the phosphatase domains, Sci Rep 2017 Aug;7(1):7875.
• 2017
  Bahloul A, Pepermans E, Raynal B, Wolff N, Cordier F, England P, Nouaille S, Baron B, El-Amraoui A, Hardelin JP, Durand D, Petit C, Conformational switch of harmonin, a submembrane scaffold protein of the hair cell mechanoelectrical transduction machinery, FEBS Lett. 2017 08;591(15):2299-2310.
• 2016
  Delhommel F, Wolff N, Cordier F, (1)H, (13)C and (15)N backbone resonance assignments and dynamic properties of the PDZ tandem of Whirlin, Biomol NMR Assign 2016 10;10(2):361-5.
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Contact

Phone:+33 1 45 68 88 72 Email:nicolas.wolff@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France

Published on: 16 Jul 2015 • Modified on: 28 May 2019