My group is interested in understanding the molecular mechanisms leading to large expansions of trinucleotide repeats involved in human disorders (Steinert disease, Huntington disease, fragile-X syndrome, etc.), using budding yeast as a model organism.
Over the recent years, we have been developing an original approach, using highly specific DNA endonucleases (TALEN, Cas nucleases) to make a double-strand break within an expanded trinucleotide repeats in order to contract it below its pathological length in humans. To that end, we use mouse and human cell lines from patients affected by these disorders.
Members
Former Members
2000
2000
Name
Position
Alix Kerrest
PhD student
Grégoire Michoud
Master student
Valentine Mosbach
PhD student
Cyril saguez
Post-doctoral fellow
Lucie Poggi
PhD student
Olivia Frenoy
Research engineer
Lisa Emmenegger
Research engineer
Wilhelm Vaysse-Zinkhöfer
PhD student
Fundings
Featured publications
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2021Differential efficacies of Cas nucleases on microsatellites involved in human disorders and associated off-target mutations., Nucleic Acids Res 2021 08; 49(14): 8120-8134.
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2021Alternative DNA Structures In Vivo: Molecular Evidence and Remaining Questions., Microbiol Mol Biol Rev 2021 Feb; 85(1): .
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2020Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay., Methods Mol Biol 2020 ; 2056(): 113-120.
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2019Trinucleotide repeat instability during double-strand break repair: from mechanisms to gene therapy., Curr Genet 2019 Feb; 65(1): 17-28.
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2018TALEN-Induced Double-Strand Break Repair of CTG Trinucleotide Repeats., Cell Rep. 2018 Feb 20;22(8):2146-2159.
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2015Shortening trinucleotide repeats using highly specific endonucleases: a possible approach to gene therapy?, Trends Genet. 2015 Apr;31(4):177-86.
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2014Highly specific contractions of a single CAG/CTG trinucleotide repeat by TALEN in yeast, PLoS ONE 2014;9(4):e95611.
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2009SRS2 and SGS1 prevent chromosomal breaks and stabilize triplet repeats by restraining recombination, Nat. Struct. Mol. Biol. 2009 Feb;16(2):159-67.
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2008Comparative genomics and molecular dynamics of DNA repeats in eukaryotes, Microbiol. Mol. Biol. Rev. 2008 Dec;72(4):686-727.
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2003Analysis of microsatellites in 13 hemiascomycetous yeast species: mechanisms involved in genome dynamics, J. Mol. Evol. 2003 Jun;56(6):730-41.
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2003Contractions and expansions of CAG/CTG trinucleotide repeats occur during ectopic gene conversion in yeast, by a MUS81-independent mechanism, J. Mol. Biol. 2003 Feb;326(3):769-82.
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2002Transcription and nuclear transport of CAG/CTG trinucleotide repeats in yeast, Nucleic Acids Res. 2002 Aug;30(16):3540-7.
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2001Microsatellite typing as a new tool for identification of Saccharomyces cerevisiae strains, J. Clin. Microbiol. 2001 Feb;39(2):551-9.
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1999Trinucleotide repeats and other microsatellites in yeasts, Res. Microbiol. 1999 Nov-Dec;150(9-10):589-602.
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Contact
Phone: +33-1-45-68-84-36 Email: gfrichar@pasteur.fr Address: 25-28 Rue du Docteur Roux 75015, Paris France