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© Research
Publication : Methods in Molecular Biology

Single-Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in Molecular Biology - 01 Apr 2022

Flora Plessier, Sandrine Schmutz, Sophie Novault, Heather Marlow

Link to Pubmed [PMID] – 35359329

Link to DOI – 10.1007/978-1-0716-2172-1_30

Methods Mol Biol 2022;2450:565-581

Cnidarians have historically served as excellent laboratory models for regenerative development given their capacity to regrow large portions of the adult organism. This capacity is notably absent or poorly developed in the powerful genetic laboratory models Drosophila, C. elegans, and mouse. Increasingly, development of genetic and genomic resources and the application of next-generation sequencing-based techniques in cnidarian systems has further expanded the potential of cnidarian regenerative models. Here, we present a workflow for the characterization of the regenerative response in the sea anemone Nematostella vectensisutilizing fluorescence-activated cell sorting and a plate-based single-cell RNA-sequencing pipeline. This approach can characterize the transcriptional response during regeneration in distinct populations of cells, thus providing a quantitative view of a whole organism process at cellular resolution.

https://pubmed.ncbi.nlm.nih.gov/35359329/