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© Institut Pasteur
Macrophages et lymphocytes de souris. Image colorisée.
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Timeline

CV

Birth date : 3/3/1987
Citizenship : French

  • EDUCATION :
    • 2011 – 2013 : Master 2 Biologie Santé Ecologie voie Pro, Ecole Pratique des Hautes Etudes (EPHE), Paris, mention Bien.
    • 2011 : Certificat d’Etudes Supérieures en Recherche BioMédicale.
    • 2007 – 2010 : BTS BioAnalyses et Contrôles, AFBB – ESTBA, Paris.
  • PROFESSIONNAL EXPERIENCES  :

2014 : Assistant engineer, Institut Cochin, INSERM U 1016, Cell cultures and dosages in microplates, multiparametric flow Cytometry (13 to 14 colors on BD LSRII), cell sortingTris cellulaires (magnetic columns and FACS on BD ARIA III), molecular screening.

Fin 2013: Platform engineer, Institut Cochin, INSERM, Immuno-biologie CYBIO.
Calibration and cytometers maintenance, support technique et formation des utilisateurs

2011-2013 : Master EPHE training, Institut Cochin, INSERM U 1016. Équipe Présentation de l’antigène par les cellules dendritiques. “Caractérisation de l’équivalent simien mDC humaines CD141+ et murines CD8a+ chez des macaques rhésus sains et infectés par le SIV”

2010-2011 : Training, Institut Cochin, INSERM U 1016. “Etude de la déplétion des cellules dendritiques myéloïdes circulantes et de l’augmentation des monocytes M-DC8+ pro-inflammatoires au cours de l’infection chronique par le VIH-1”

BTS training, Université René DESCARTES. “Etude de la cytotoxicité de candidats médicaments dans le traitement de la maladie de Wilson”

  • SCIENTIFIC SKILLS 

Microbiology : isolations, seedings, identifications, antibiograms, cell enumerations
Biochemistry : Titrages, chromatographies, spectrophotométrie, électrophorèses, Dosages enzymatiques, PCR
Immunology : sera and hemolytic toxins titration, serum typing, ELISA, ELISPOT, CBA (Cytometric Beads Array), immunological labeling for phenotypical characterisation of cells and intra-membrane labeling
Diverses : Cell culture and dosages in microplates, multiparametric flow cytometry Cytométrie(13 to 14 coulors on BD LSRII), cell sortings (magnetic columns and FACS on BD ARIA III)

  • PUBLICATIONS

April 16,  2014: TLR3-Responsive, XCR1+, CD141 (BDCA-3)+/CD8 α+-Equivalent Dendritic Cells Uncovered in Healthy and Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Immunol
Dutertre CA1, Jourdain JP, Rancez M, Amraoui S, Fossum E, Bogen B, Sanchez C, Couëdel-Courteille A, Richard Y, Dalod M, Feuillet V, Cheynier R, Hosmalin A.
2014 May 15;192(10):4697-708.
doi: 10.4049/jimmunol.1302448.
Epub 2014 Apr 16.
PMID : 24740505

July 16, 2012 : Dutertre, C.A., Amraoui, S., DeRosa, A., Jourdain, J.P., Vimeux, L., Goguet, M., Degrelle, S., Feuillet, V.,Liovat, A.S., Müller-Trütwin, M., Decroix, N., Deveau, C., Meyer, L., Goujard, C., Loulergue, P., Launay, O., Richard, Y. et Homalin, A. (2012).
Pivotal role of M-DC8+ monocytes from viremic HIV-infected patients in TNF over-production in response to microbial products. Blood,
2012 Sep 13;120(11):2259-68. : 10.1182/blood-2012-03-418681.
Epub 2012 Jul 16. PMID : 22802339

  • LANGUAGES

English : Bilingual
German : Professional

  • INFORMATIC SKILLS

Office: Microsoft Office Word, Excel, Powerpoint
Infography: Adobe Photoshop
Data bases: NCBI, EBI, Blast, PDB, FASTA Bibliographie PubMed, EndNote
Flow cytometry: BD FACS Diva, FlowJo