I am interested in the molecular events that control the entry of the bacterial pathogen Listeria monocytogenes within mammalian host cells. In particular, I investigate the signaling pathways that are activated downstream of the adhesion molecule E-cadherin and the hepatocyte growth factor receptor Met, which are the cellular receptors for the L. monocytogenes surface proteins InlA and InlB. Applying a systems biology strategy, my team has recently analysed diverse siRNA, microRNA and drug libraries to identify novel signaling networks that up- or down-regulate L. monocytogenes infection. At present, we are studying the function of these novel networks not only within an infection context, but also from a broader cellular biology perspective by using L. monocytogenes as a molecular tool to manipulate host cell functions. Finally, I am also interested in the characterization of novel bacterial virulence factors present only in epidemic L. monocytogenes strains, including the first bacteriocin reported for this species which modulates the host microbiota to favor infection.
Department of Microbiology, co-organized with Cell Biology & Infection “Black Spot, Black Death, Black Pearl: The Tales of Bacterial Effectors”, by Prof. Kim ORTH
Modeling the Mammalian Microbiota Host Superorganism – www.mam-2018.org
Systems biology of cell infection by the bacterial pathogen Listeria monocytogenes
In the context of the Swiss consortium InfectX (www.infectx.ch), we have previously performed siRNA, microRNA and drug screens to investigate signaling pathways modulating invasion of host cells by the bacterial pathogen Listeria monocytogenes. In […]
Host microbiota modification by epidemic Listeria monocytogenes
Listeriolysin S is a bacteriocin specifically produced in the intestine of orally infected mice and necessary for Listeria monocytogenes survival in this organ. In order to investigate whether this toxin might influence the composition […]
Welcome to the club! Welcome to Microfluidics@Pasteur! Our goal is to serve as the major entry point to all the scientists on the campus (beginners and experts) interested in undertaking projects where microfluidics is involved. Our […]
PTR521-2015 microRNAs & Listeria
In collaboration with the swiss consortium InfectX (https://www.infectX.ch) we have previously performed high through-put microRNA screens to identify novel gene networks modulating the infection of host cells by Listeria. We are now : 1) […]
Infectious diseases are a major public health problem, and identification of novel potentialtherapeutic candidates is mandatory in a context of growing antibiotic resistance. As classical infection biology approaches are nearly exhausted in their current […]
Diversity of Listeria spp. in Costa Rica
Incidence of listeriosis in Costa Rica or Central America has not been previously studied. The only listeriosis outbreak reported in Costa Rica was described in 1989 (Schuchat et al., 1991). Nevertheless, the Costa Rican […]
Dual microscopy assay to discriminate Listeria cellular entry & vacuolar escape
We developed a novel image-based microscopy assay which allows to discriminate Listeria monocytogenes cellular entry from vacuolar escape, enabling high-content screening to identify factors specifically involved in these two steps. We generated a L. […]
InlC as a tool to label late cellular infection stages by Listeria
Bacterial intracellular pathogens can be conceived as molecular tools to dissect cellular signaling cascades due to their capacity to exquisitely manipulate and subvert cell functions which are required for the infection of host target […]
Medium-Throughput Gentamicin Invasion Assay
This gentamicin invasion assay protocol to measure bacterial invasion of mammalian in vitro cultures is compatible with medium-throughput small interfering RNA screens in 96-well plates (Kühbacher et al. 2014. Internalization Assays for Listeria monocytogenes. Methods in […]
Javier Pizarro-Cerda obtained B.Sc. and M.Sc. degrees from the University of Costa Rica while working in the laboratory of Edgardo Moreno, studying the adaptations of the outer membrane of the Gram-negative pathogen Brucella abortus to cationic peptides. He then obtained a D.E.A. and a Ph.D. from the University of Aix-Marseilles while working in the laboratory of Jean-Pierre Gorvel, identifying the intracellular trafficking of Brucella abortus in epithelial cells. Subsequently, he joined the laboratory of Pascale Cossart at the Pasteur Institute in Paris to investigate signaling cascades triggered by the Gram-positive pathogen Listeria monocytogenes during invasion of host mammalian cells. In 2001 Javier Pizarro-Cerda joins the Pasteur Institute as a permanent researcher and obtains his first grant from the French Ministry of Research. Since, he has been involved in local (Pasteur Institute Transversal Programs), national (National Agency for Research) and international (ERANET, System X) research initiatives, investigating the adaptations of bacterial pathogens to intracellular life. He has been actively involved in teaching and has participated in practical and theoretical courses in France, Argentina, Costa Rica, Grèce and China. During his career, Javier Pizarro-Cerda has developed his research as invited investigator in different international laboratories including the Center for Microscopy & Microanalysis (Queensland University, Australia), the Weizmann Institute (Rehovot, Israel), the University of Texas-Southwestern (Dallas, USA), the Max Planck Institute for Infection Biology (Berlin, Germany) and the Biozentrum (Basel, Switzerland). In 1998, Javier Pizarro-Cerda obtains the Costarrican National Award for Science ‘Clodomiro Picado-Twight’, in 2012 he becomes member of the Costarrican Academy of Sciences and in 2015 he receives the Senate Medal from the French Senate for his contribution fostering interactions between France and Latin America. In 2016, Javier Pizarro-Cerda becomes head of the ‘Systems Biology of Bacterial Infections’ Group and is promoted as Director of Research at the Pasteur Institute, while in 2017 he receives the ‘Canetti Prize’ from the Pasteur Institute Scientific Council for his global contributions to the field of infectious diseases.
2018Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic Listeria monocytogenes using the chicken embryo infection model, Vet. Res. 2018 Feb;49(1):13.
2018Listeria costaricensis sp. nov, Int. J. Syst. Evol. Microbiol. 2018 Jan;.
2017Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles, Nat Commun 2017 Nov;8(1):1396.
2017Listeriolysin S Is a Streptolysin S-Like Virulence Factor That Targets Exclusively Prokaryotic Cells In Vivo, MBio 2017 Apr;8(2).
2017Listeriolysin S: a bacteriocin from epidemic Listeria monocytogenes strains that targets the microbiota, Gut Microbes 2017 Feb;.
2017The Diverse Family of Arp2/3 Complexes, Trends Cell Biol. 2017 Feb;27(2):93-100.
2017Assessing Vacuolar Escape of Listeria Monocytogenes, Methods Mol. Biol. 2017;1535:173-195.
2016Role of Listeria monocytogenes exotoxins in virulence, Microbial Toxins 2016 Sep;1-20.
2016Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia, Semin. Cell Dev. Biol. 2016 Jul;.
2016Cell Biology and Microbiology: A Continuous Cross-Feeding, Trends Cell Biol. 2016 May;.
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