I am interested in the molecular events that control the entry of the bacterial pathogen Listeria monocytogenes within mammalian host cells. In particular, I investigate the signaling pathways that are activated downstream of the adhesion molecule E-cadherin and the hepatocyte growth factor receptor Met, which are the cellular receptors for the L. monocytogenes surface proteins InlA and InlB. Applying a systems biology strategy, my team has recently analysed diverse siRNA, microRNA and drug libraries to identify novel signaling networks that up- or down-regulate L. monocytogenes infection. At present, we are studying the function of these novel networks not only within an infection context, but also from a broader cellular biology perspective by using L. monocytogenes as a molecular tool to manipulate host cell functions. Finally, I am also interested in the characterization of novel bacterial virulence factors present only in epidemic L. monocytogenes strains, including the first bacteriocin reported for this species which modulates the host microbiota to favor infection.
LabEx IBEID – Integrative Biology of Emerging Infectious Diseases
Presentation The aim of the Integrative Biology of Emerging Infectious Diseases (IBEID) project, coordinated by Professors Philippe Sansonetti and Pascale Cossart, is to develop a structure to anticipate and tackle emerging infectious diseases (EIDs). […]
What’s best to study humans and their infectious deseases than Human emulation system ? It’s now possible at Pasteur to develop real Organs-on-chips with cells from different sources, patient derived or cell line. At […]
Systems biology of cell infection by the bacterial pathogen Listeria monocytogenes
In the context of the Swiss consortium InfectX (www.infectx.ch), we have previously performed siRNA, microRNA and drug screens to investigate signaling pathways modulating invasion of host cells by the bacterial pathogen Listeria monocytogenes. In […]
Host microbiota modification by epidemic Listeria monocytogenes
Listeriolysin S is a bacteriocin specifically produced in the intestine of orally infected mice and necessary for Listeria monocytogenes survival in this organ. In order to investigate whether this toxin might influence the composition […]
Welcome to the club! Welcome to Microfluidics@Pasteur! Our goal is to serve as the major entry point to all the scientists on the campus (beginners and experts) interested in undertaking projects where microfluidics is involved. Our […]
PTR521-2015 microRNAs & Listeria
In collaboration with the swiss consortium InfectX (https://www.infectX.ch) we have previously performed high through-put microRNA screens to identify novel gene networks modulating the infection of host cells by Listeria. We are now : 1) […]
Infectious diseases are a major public health problem, and identification of novel potentialtherapeutic candidates is mandatory in a context of growing antibiotic resistance. As classical infection biology approaches are nearly exhausted in their current […]
Diversity of Listeria spp. in Costa Rica
Incidence of listeriosis in Costa Rica or Central America has not been previously studied. The only listeriosis outbreak reported in Costa Rica was described in 1989 (Schuchat et al., 1991). Nevertheless, the Costa Rican […]
Dual microscopy assay to discriminate Listeria cellular entry & vacuolar escape
We developed a novel image-based microscopy assay which allows to discriminate Listeria monocytogenes cellular entry from vacuolar escape, enabling high-content screening to identify factors specifically involved in these two steps. We generated a L. […]
InlC as a tool to label late cellular infection stages by Listeria
Bacterial intracellular pathogens can be conceived as molecular tools to dissect cellular signaling cascades due to their capacity to exquisitely manipulate and subvert cell functions which are required for the infection of host target […]
Medium-Throughput Gentamicin Invasion Assay
This gentamicin invasion assay protocol to measure bacterial invasion of mammalian in vitro cultures is compatible with medium-throughput small interfering RNA screens in 96-well plates (Kühbacher et al. 2014. Internalization Assays for Listeria monocytogenes. Methods in […]
Javier Pizarro-Cerda obtained B.Sc. and M.Sc. degrees from the University of Costa Rica while working in the laboratory of Edgardo Moreno, studying the adaptations of the outer membrane of the Gram-negative pathogen Brucella abortus to cationic peptides. He then obtained a D.E.A. and a Ph.D. from the University of Aix-Marseilles while working in the laboratory of Jean-Pierre Gorvel, identifying the intracellular trafficking of Brucella abortus in epithelial cells. Subsequently, he joined the laboratory of Pascale Cossart at the Pasteur Institute in Paris to investigate signaling cascades triggered by the Gram-positive pathogen Listeria monocytogenes during invasion of host mammalian cells. In 2001 Javier Pizarro-Cerda joins the Pasteur Institute as a permanent researcher and obtains his first grant from the French Ministry of Research. Since, he has been involved in local (Pasteur Institute Transversal Programs), national (French National Agency for Research, Defense Innovation Agency) and international (ERANET, Systems X, National Institutes of Health) research initiatives, investigating the adaptations of bacterial pathogens to intracellular life. He has been actively involved in teaching and has participated in practical and theoretical courses in France, Argentina, Costa Rica, Grèce and China. During his career, Javier Pizarro-Cerda has developed his research as invited investigator in different international laboratories including the Center for Microscopy & Microanalysis (Queensland University, Australia), the Weizmann Institute (Rehovot, Israel), the University of Texas-Southwestern Medical Center (Dallas, USA), the Max Planck Institute for Infection Biology (Berlin, Germany) and the Biozentrum (Basel, Switzerland). In 1998, Javier Pizarro-Cerda obtains the Costarrican National Award for Science ‘Clodomiro Picado-Twight’, in 2012 he becomes member of the Costarrican Academy of Sciences and in 2015 he receives the High Chamber Medal from the French Senate for his contribution fostering interactions between France and Latin America. In 2016, Javier Pizarro-Cerda becomes head of the ‘Systems Biology of Bacterial Infections’ Group and is promoted as Research Director at the Pasteur Institute. In 2017 he receives the ‘Canetti Prize’ from the Pasteur Institute Scientific Council for his global contributions to the field of infectious diseases and becomes head of the Yersinia Research Unit as well as Director of the World Health Organization (WHO) Collaborating Reference and Research Centre for Yersinia. In 2018 he becomes member of the WHO International Roster of Experts (Plague Panel) while in 2019 Javier Pizarro-Cerda is admited as member of the Academia Europaea.
2019Can we make human plague history? A call to action, BMJ Global Health 2019;4:e001984. doi:10.1136/ bmjgh-2019-001984.
2019Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine, bioRxiv 2019, 822221.
2019Bread feeding is a robust and more physiological enteropathogen administration method compared to oral gavage, bioRxiv 2019, 808832.
2019A Listeria monocytogenes Bacteriocin Can Target the Commensal Prevotella copri and Modulate Intestinal Infection, Cell Host Microbe 2019 11;26(5):691-701.e5.
2019Philippe Sansonetti and Cellular Microbiology, Cell. Microbiol. 2019 Oct;:e13134.
2019Genus-wide Yersinia core-genome multilocus sequence typing for species identification and strain characterization, Microb Genom 2019 Oct;.
2019Reassessing the role of internalin B in Listeria monocytogenes virulence using the epidemic strain F2365, Clin. Microbiol. Infect. 2019 Feb;25(2):252.e1-252.e4.
2019Yersinia pestis and plague: an updated view on evolution, virulence determinants, immune subversion, vaccination and diagnostics, Microbes Infect. 2019 Jun;.
2019Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging, Methods Mol Biol. 2019;2010:85-97..
2019Microbe Profile: Listeria monocytogenes: a paradigm among intracellular bacterial pathogens, Microbiology (Reading, Engl.) 2019 Jul;165(7):719-721.
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