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© Research
Project

Microinjection of “External” Mutant ES Cells And Subsequently Germ-Line Chimera Generation

Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
26
Jan 2015
Status
Ongoing
Members
4
Structures
1

About

The easy availability of modified ES cells obtained from KOMP and EUCOMM high-throughput gene-targeting mutagenesis programs (mainly JM8.N4, JM8 A3.N1 ES cell lines) has considerably increased the requests for these direct microinjections in the platform. As for the “CIGM internal mutant ES cells”, the external ES mutant cells are microinjected into appropriated mouse blastocysts (mainly BALB/cN) to generate mouse chimeras which will give rise to the desired mutant heterozygous mice.