I am interested in the discovery of microbial factors and host mechanisms playing a critical role in the pathogenesis of infectious diseases and whose characterization could help develop new diagnostic and therapeutic tools. I focus most of my research activities on the identification of new virulence mechanisms of the model pathogen Listeria monocytogenes. I have been involved in the first comparative genomic analysis of L. monocytogenes and the non pathogenic species Listeria innocua. This genomic approach led to the identification of many new bacterial factors necessary for efficient colonization of the host, including a bile salt hydrolase required for resistance to bile and a serine/threonine phosphatase regulating the superoxide dismutase antioxidant activity and the elongation factor EF-Tu. Our current research is aimed at understanding the strategies evolved by Listeria to subvert the host immune response and to spread within the host, using a combination of genomic and in vivo transcriptomic approaches.
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Inhibitors of bacterial NAD kinases
Sylvie Pochet (PI)
NAD mimics as potential antibacterial agents The NAD(P) biosynthesis pathways have attracted recent interest as a source of enzyme targets for the development of antimicrobial agents. Our own research focused on the structure-based development […]
2019Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging, Methods Mol Biol. 2019;2010:85-97..
2019Yersinia pestis and plague: an updated view on evolution, virulence determinants, immune subversion, vaccination, and diagnostics, Genes Immun. 2019 Apr;.
2017Listeriolysin S Is a Streptolysin S-Like Virulence Factor That Targets Exclusively Prokaryotic Cells In Vivo, MBio 2017 Apr;8(2).
2014The bacterial pathogen Listeria monocytogenes and the interferon family: type I, type II and type III interferons, Front Cell Infect Microbiol 2014;4:50.
2012The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection, PLoS ONE 2012;7(8):e43455.
2012Screening and in situ synthesis using crystals of a NAD kinase lead to a potent antistaphylococcal compound, Structure 2012 Jun;20(6):1107-17.
2012Both TLR2 and TRIF contribute to interferon-β production during Listeria infection, PLoS ONE 2012;7(3):e33299.
2011OatA, a peptidoglycan O-acetyltransferase involved in Listeria monocytogenes immune escape, is critical for virulence, J. Infect. Dis. 2011 Sep;204(5):731-40.
2011Recruitment of the major vault protein by InlK: a Listeria monocytogenes strategy to avoid autophagy, PLoS Pathog. 2011 Aug;7(8):e1002168.
2011Identity, regulation and in vivo function of gut NKp46+RORγt+ and NKp46+RORγt- lymphoid cells, EMBO J. 2011 Jul;30(14):2934-47.
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