Présentation
Successful extension of mass spectrometry-based (MS) proteomics to single cells is very challenging, mainly due to low analysis throughput and insufficient proteome coverage in single cells. To address these challenges, new pipelines have emerged that combine microfluidic nanodroplet (nanoPoTS: Zhu Y, Nat Commun, 2018) or flow cytometry (SCOPED-MS: Budnik B, Gen Biol, 2018; Specht H, BiorXiv, 2019) with recent quantitative proteomics approaches, such as Tandem Mass TagTM (TMT) isobaric labeling.
In an ongoing collaborative project with the Proteomics platform of the Institut Curie, the Institut Pasteur Proteomics Platform works on implementing recently established high-throughput methods for single cell proteomic analysis to the campus. Moreover, new pipelines developed in the framework of the “Emerging and Future Proteomics Technologies” Joined Research Activity (JRA) coordinated by the UTechS MSBio within of the EPIC-XS consortium will be transferred to the Institut Pasteur. EPIC-XS is a European infrastructure gathering 18 European leading groups in proteomics, including the MSBio Unit at the Institut Pasteur for France. The Proteomics Platform is open to collaborations with the scientific community to establish and apply innovative single-cell proteomics approaches.
In addition, ultrasensitive immuno-assay-based technologies are available at the CB UTechS to identify single-cell protein signatures of stimulated cells.
Technologies
SiMoA (Quanterix). Based on ultrasensitive, digital ELISA, the instrument enables quantification of 1-9 analytes in biological liquids or cell lysates with pM sensitivity, from low sample volumes
SP-X (Quanterix). Based on ultrasensitive, digital ELISA, like SiMoA, with a higher multiplexing capacity.