In the Ghigo laboratory, the activity of the group focuses on two biofilm-specific phenomena: initial adhesion to surfaces or between bacteria – a key step of any colonization process, and biofilms recalcitrance towards antibiotic , a major biofilm behavior responsible for chronic and recurrent infections. Beyond fundamental aspects of understanding the molecular mechanisms controlling these two biofilm-properties , these studies also display potential for translation to clinically relevant situations involving biofilms.
PTR OmNega: A multi-disciplinary investigation of the Negativicutes: atypical Firmicutes with LPS-outer membranes that inhabit the human gut
The Negativicutes are a poorly studied lineage of bacteria that include common inhabitants of the human oral and gut microbiome such as the anaerobe Veillonella, and which can also develop into opportunistic pathogens. Despite […]
Commensal and pathogenic bacteria such as Escherichia coli produce various surface structures promoting their interaction with living or inert surfaces. We study the structure and regulation of E. coli adhesins and extracellular matrix polysaccharides contributing to biofilm […]
Biofilm Tolerance to Antibiotics
One of the of the hallmarks of biofilm physiology is its tolerance to a high level of antimicrobials. We are currently investigating the molecular mechanisms that lead to this transient ability to withstand antimicrobial treatments. […]
Biofilm formation on medical devices is associated with hospital-acquired (nosocomial) infections. Due to biofilms’ high tolerance to antibiotics, classical treatments lack efficacy, and removal of the biofilm-contaminated device is often the only therapeutic option. […]
Biophysics of Bacteria-Surface Interactions
Biofilm formation relies as much on bacterial adhesion properties than on surface chemistry, and many questions remain regarding biophysics of surface adhesion. In collaboration with physico-chemists and biophysicists, we used multidisciplinary approaches to investigate bacterial […]
2018Increased osmolarity in biofilm triggers RcsB-dependent lipid A palmitoylation in Escherichia coli, MBio. 2018 Aug 21;9(4). pii: e01415-18. doi: 10.1128/mBio.01415-18..
2018Probing the influence of cell surface polysaccharides on nanodendrimer binding to Gram-negative and Gram-positive bacteria using single-nanoparticle force spectroscopy, Nanoscale. 2018 Jul 9;10(26):12743-12753. .
2018Asymetric adhesion in rod-shaped bacteria controls microcolony morphogenesis, Nat Commun. 2018 Mar 16;9(1):1120. doi: 10.1038/s41467-018-03446-y.
2017Comparative Analysis of Bacterial Community Composition and Structure in Clinically Symptomatic and Asymptomatic Central Venous Catheters, mSphere 2017 Sep-Oct;2(5).
2017YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation, Sci Rep. 2017 Sep 12;7(1):11326. doi: 10.1038/s41598-017-10902-0..
2017Long-term stability of gentamicin sulfate-ethylenediaminetetraacetic acid disodium salt (EDTA-Na2) solution for catheter locks, J. Pharm. Anal. 2017 in press.
2017Outer Membrane Proteome of Veillonella parvula: A Diderm Firmicute of the Human Microbiome, Front. Microbiol., 30 June 2017, 8:6979-17.
2017Central venous catheters and biofilms: where do we stand in 2017?, APMIS. 2017 Apr;125(4):365-375. doi: 10.1111/apm.12665..
2017Understanding biofilm formation in intravascular device-related infections, Intensive Care Medicine. Mar;43(3):443-446. doi: 10.1007. [Epub 2016 Aug 6].
2016Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes, Elife. 2016 Aug 31;5. pii: e14589..
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