The flow cytometry facility organizes various courses in flow cytometry every year. We offer basic training to users for a range of instruments, assays, and software. Our courses involve the fundamental aspects of flow cytometry and its applications and there are practical sessions. The facility also offers tailor-made training courses.
Registered users can see all training request types in PPMS: https://ppms.eu/pasteur/login/?pf=10
♦ Basics of Cytometry TRAINING
This training is addressed to those who are new to flow cytometry and want to understand the basics of the technology before attending instrument operating training.
The course starts with an introduction to this quickly evolving technology, its importance and the resources that are available. One of the main goals of this course is to give the participants a firm understanding of fluorescence, fluorochromes and the working of a cytometer. This theoretical part is followed by a practical session on the instrument, focused on experiment set up, fluorescence compensations, basics of experimental design and reporting of your flow cytometry data.
This training is mandatory for all new users in flow cytometry. It is also highly recommended that all new users with low experience in flow cytometry attend this training before attending other training modules. This confirms that the basics of flow cytometry have been grasped before going to more advanced topics and trainings.
This 1-day course covers the fundamentals of the technology and builds up the knowledge of the delegates through a series of lectures, exercises, practical examples, and interactive learning.
The learning objectives of this training are :
– Gain an overview of the technology and its applications
– Understand how a cytometer works
– Recognize the need for good sample preparation
– Understand the principle of fluorescence compensation
– Be able to design a simple flow cytometry experiment, and understand the importance of fluorochrome choice
– Have a greater understanding on how to optimize your results
– Be able to communicate your results to your peers
♦ CytoFlex S TRAINING
We provide training for operating our 4-laser 13-parameter cytometers.
Details: instrument training provided upon request.
Prerequisite: basics of cytometry training.
Duration: 4hr of theoretical/ hands on session.
Location: Flow Cytometry Platform (FJ level 1).
Register: facility users can request training using the PPMS training request form.
♦ BD Fortessa/ Symphony A5 TRAINING
We provide training for operating our 5-laser 18 or 30-parameter cytometers.
Details: instrument training provided upon request.
Prerequisite: basics of cytometry training.
Duration: 2hr of theoretical session, 1 day of hands on session.
Location: Flow Cytometry pf (FJ level 1).
Register: facility users can request training using the PPMS training request form.
♦ BD AriaIII/ Fusion/Symphony S6 TRAINING
We provide training for operating our 5-laser 18 or 30-parameters cell sorters.
Details: instrument training provided upon request.
Prerequisite: basics of flow cytometry, DIVA software, PMTs & compensation settings, Panel design, experiment set up.
Duration: 2hr of theoretical session, 1 day of hands on session.
Location: Flow Cytometry pf (FJ level 1).
Register: facility users can request training using the PPMS training request form.
♦ FlowJO basic TRAINING
We provide training for various kinds of analysis software, including FlowJo analysis of flow cytometry data.
Prerequisite: FlowJo tutorials and online webinars.
Duration: 1 day of hands on session.
Location: Pasteur n°20.
Register: facility users can request training using the PPMS training request form.
♦ FlowJO plugins TRAINING
We provide training for unsupervised analysis by using R packages
Details: instrument training provided upon request.
Prerequisite: flowJO basics.
Duration: 1 day of hands on session.
Location: Pasteur n°20.
Register: facility users can request training using the PPMS training request form.
♦ Kaluza TRAINING
We provide training for various kinds of analysis software, including FlowJo analysis of flow cytometry data.
Prerequisite: FlowJo tutorials and online webinars.
Duration: 1 day of hands on session.
Location: Flow Cytometry pf (FJ level 1).
Register: facility users can request training using the PPMS training request form.
