Bacteria are remarkable models for biology and have phenotypic characteristics of great cognitive, health or biotechnological values. Most bacteria are constantly exposed to fluctuating environments, thus are ideally set to detect, react and adapt to various stress conditions. The Pasteur unit “Adaptation to stress and metabolism of enterobacteria” aims to understand the stress response at the populational, cellular and molecular level. Our unit comprises 12 scientists, including Pasteur and CNRS researchers , engineers and technicians, post-docs and students.
We study how a wide range of basic functional processes are modified, and possibly coordinated, to control cellular homeostasis. Our research mainly uses E. coli as a model, but concepts will be as well tested on pathogens such as Salmonella or Shigella. Focusing on two global cellular processes, Fe-S cluster biogenesis and lipid homeostasis, allows us to study the impact of stress on aerobic and anaerobic metabolism, cell envelope and membrane homeostasis, redox changes, limitation of nutrients, metabolic and antibiotics stresses.
Molecular, biochemical and genetic approaches, as well as cutting-edge technologies (-omics, imaging, single-cell analysis), aim to achieve an integrated and mechanistic vision of the bacterial cell. To multiply and diversify the ways to approach a process is very rewarding, and we collaborate with biochemists, structuralists, chemists, biophysicists, bioinformaticians and phylogeneticists.
Members
Former Members
2000
2000
Name
Position
2018
2020
Alessandra Lo Sciuto
Post-doc
Transversal Project
Publications
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2021Iron-sulfur biology invades tRNA modification: the case of U34 sulfuration., Nucleic Acids Res 2021 Mar; (): .
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2020Oxidative stress antagonizes fluoroquinolone drug sensitivity via the SoxR-SUF Fe-S cluster homeostatic axis., PLoS Genet 2020 Nov; 16(11): e1009198.
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2020Quantification of guanosine triphosphate and tetraphosphate in plants and algae using stable isotope-labelled internal standards., Talanta 2020 Nov; 219(): 121261.
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2020The O2-independent pathway of ubiquinone biosynthesis is essential for denitrification in Pseudomonas aeruginosa., J Biol Chem 2020 Jul; 295(27): 9021-9032.
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2020Making iron-sulfur cluster: structure, regulation and evolution of the bacterial ISC system., Adv Microb Physiol 2020 ; 76(): 1-39.
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2020SlyA Transcriptional Regulator Is Not Directly Affected by ppGpp Levels., Front Microbiol 2020 ; 11(): 1856.
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2020Deciphering the specific interaction between the acyl carrier protein IacP and the T3SS-major hydrophobic translocator SipB from Salmonella., FEBS Lett 2020 01; 594(2): 251-265.
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2019RSH enzyme diversity for (p)ppGpp metabolism in Phaeodactylum tricornutum and other diatoms., Sci Rep 2019 11; 9(1): 17682.
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2019The Varied Role of Efflux Pumps of the MFS Family in the Interplay of Bacteria with Animal and Plant Cells, Microorganisms 2019 Aug;7(9).
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2019The SUF system: An ABC ATPase-dependent protein complex with a role in Fe-S cluster biogenesis, Res. Microbiol. 2019 Aug;.
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