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Scientific Fields
Diseases
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Published in Journal of cell science - 22 Jun 2020

Rosazza T, Lecoeur H, Blisnick T, Moya-Nilges M, Pescher P, Bastin P, Prina E, Späth GF,

Link to Pubmed [PMID] – 32501279

Link to DOI [DOI] – jcs24277610.1242/jcs.242776

J Cell Sci 2020 Jun; 134(5):

Leishmania spp. are obligate intracellular parasites that infect phagocytes, notably macrophages. No information is available on how Leishmania parasites respond to pyroptosis of their host cell, which is known to limit microbial infection. Here, we analyzed the pyroptotic process and the fate of intracellular amastigotes at the single-cell level using high-content real-time imaging. Bone marrow-derived macrophages were infected with virulent Leishmania amazonensis amastigotes and sequentially treated with lipopolysaccharide and ATP to induce pyroptosis. Real-time monitoring identified distinct pyroptotic phases, including rapid decay of the parasitophorous vacuole (PV), progressive cell death and translocation of the luminal PV membrane to the cell surface in 40% of macrophages, resulting in the extracellular exposure of amastigotes that remained anchored to PV membranes. Electron microscopy analyses revealed an exclusive polarized orientation of parasites, with the anterior pole exposed toward the extracellular milieu, and the parasite posterior pole attached to the PV membrane. Exposed parasites retained their full infectivity towards naïve macrophages suggesting that host cell pyroptosis may contribute to parasite dissemination.

https://pubmed.ncbi.nlm.nih.gov/32501279