Dorian Obino

Born and raised in the south of France, I moved to Paris to study at the Magistère Européen de Génétique (Université Paris VII, now Université Paris Cité) where I specialized in immuno-genetics and cell biology. I then joined the lab of Ana-Maria Lennon-Duménil at Institut Curie (Paris, France) for my PhD, where I studied the cellular and molecular mechanisms governing B lymphocyte polarization, their involvement in the regulation of antigen extraction and presentation, as well as the impact played by the lymphoid microenvironment in tuning these functions. As a postdoctoral fellow, I joined Guillaume Duménil’s lab at Institut Pasteur (Paris, France) to decipher the innate immune response to meningococcal intravascular infections, focusing on the role of neutrophils and macrophage subsets. Since October 2022, I have been recruited as a Permanent Research Scientist at INSERM to explore the function of perivascular macrophages at the blood-tissue interface and uncover their roles in regulating skin health and disease.

EDUCATION

• PhD in Immuno-cell biology – Institut Curie, INSERM U932, Paris, France (2016)
• Magistère Européen de Génétique – Major: Immuno-genetics – Universités Paris VII & Paris V, Paris, France (2009-2012)
• Master in Genetics – Université Paris VII (Paris, France), (2010-2012)
• Bachelor (Licence) in Genetics – Université Paris VII, Paris, France (2009-2010)
• DUT (Diplôme Universitaire de Technologie) in Génie Biologique – Université du Sud Toulon Var (2007 – 2009)

RESEARCH EXPERIENCE

• Since October 2022: INSERM Research Scientist (Chargé de Recherche CRCN – CSS3) – Institut Pasteur, Paris, France, Pathogenesis of Vascular Infections unit, INSERM U1225, Head: Dr. Guillaume Duménil
• 2017-2022: Postdoctral Fellow – Institut Pasteur, Paris, France, Pathogenesis of Vascular Infections unit, INSERM U1225, Head: Dr. Guillaume Duménil
• 2012-2016: PhD Student – Institut Curie, Paris, France – INSERM U932, Head: Dr. Ana-Maria Lennon-Duménil
• Ap-Aug 2011: Trainee – St Jude Children’s Research Hospital, Memphis, TN, USA – Lab of Dr. Peter J. Murray
• June-Aug 2010: Trainee – Institut Jacques Monod, Paris, France – UMR 7592, Head: Pr. Catherine Alcaïde-Loridan, Supervisor: Dr. Frédérique Deshayes

FELLOWSHIP

• 2018-2020: Pasteur-Roux Postdoctoral Fellowship – Institut Pasteur Paris
• 2015-2016: 4^th year PhD Fellowship – Fondation pour la Recherche Médicale
• 2012-2015: PhD Fellowship – BioSPC Graduate School, Universités Paris V & VII

AWARDS

• 2016: Académie Nationale de Pharmacie, Prix de Thèse en Sciences Biologiques

COMMUNICATIONS

Invited speaker

• 2023: Institut de Recherche Saint Louis, Paris, France – “Innate immune response to meningococcal intravascular infection”
• 2018: Seoul National University, Seoul, Republic of Korea – “Development of a vessels-on-a-chip device to study meningococcal intravascular infection“
• 2016: Plenary session of the Académie Nationale de Pharmacie, Paris, France – “Molecular mechanisms regulating B lymphocyte polarization”

Oral presentations selected from abstract

• 2020: Departmental seminar Cell Biology & Infection, Online – “Dermal Lyve-1⁺ perivascular macrophages: Origin & functions in health & disease”
• 2016: Journées Scientifiques et Médicales, Institut Curie, Paris, France – “Elevator talk: My PhD in 180 seconds”
• 2016: 7^th Edition of the YRLS (Young Researchers in Life Science) Conference, Paris, France – “Actin nucleation at the centrosome controls lymphocyte polarity”
• 2013: SFI (French Society of Immunology) annual meeting, Paris, France – “Galectin-8 promotes efficient antigen processing and presentation by B lymphocytes”

Poster presentations

• 2019: Departmental retreat Cell Biology & Infection, San Feliu de Guíxols, Spain – “Dermal perivascular macrophages sample blood vessels in search of pathogens”
• 2015: Actin and microtubule cytoskeleton in cell motility and morphogenesis: an integrated view. J. Monod conference, Roscoff, France – “Actin nucleation at the centrosome controls lymphocyte polarity”
• 2013: SFI (French Society of Immunology) annual meeting, Paris, France – “Galectin-8 promotes efficient antigen processing and presentation by B lymphocytes”
• 2012: Current Trends in Biochemistry, Biophysics and Cell Biology, 39^th Winter School of Faculty of Biochemistry, Biophysics and Biotechnology, Zakopane, Poland – “Tuning B lymphocyte responses through cell polarity and lysosome exocytosis”

TEACHING   

• 2022: Scientific advisor for students – Double diplôme “Politique et identité du vivant” Sciences Po – Université Paris Cité – Licence Science du Vivant. Chair: F. Deshayes
• 2018, 2019 & 2020: Co-organizer & Teaching of the Practicum: “How to make a tube” – Master 2 Pasteur Course: Molecular Biology of the Cell, Chairs: C. Zurzolo & R. Bruzzone
• 2016 & 2017: Invited lecturer – Master2 “Biologie Cellulaire, Physiologie et Pathologie, Spécialité Biologie et Développement Cellulaires”, Course: Membrane dynamics and signaling, Université Paris Descartes, Chair: A. Benmerah
• 2015 & 2016: Teaching assistant – Bachelor’s degree, Practical course: Molecular biology, Université Paris VII,  Chair: V. Joliot
• 2013 & 2014: Invited lecturer – Bachelor’s degree, Course: Cell Biology, Magistère Européen de Génétique, Université Paris VII, Chair: C. Alcaïde-Loridan

PUBLICATIONS

Adé K*, Saenz Coronilla J*, Obino D, Weinberger T, Kaiser C, Mella S, Chen C, Katsimpardi L, Werts C, Li H, Dardenne P, Lallemand Y, Gomez Perdiguero E. Inflammation drives age-induced loss of tissue resident macrophages. BioRxiv (2022).10.02.510513.

Saez JJ, Diaz J, Ibanez J, Bozo JP, Cabrera Reyes F, Alamo M, Gobert FX, Obino D, Bono MR, Lennon-Dumenil AM, Yeaman C, Yuseff MI. The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells. Cell Biol. (2019). 218(7):2247-64.

Inoue D*, Obino D*, Pineau J, Farina F, Gaillard J, Guerin C, Blanchoin L, Lennon-Dumenil AM, Thery M. Actin filaments regulate microtubule growth at the centrosome. EMBO J. (2019). 38(11). *equal contribution

Obino D, Fetler L, Soza A, Malbec O, Saez JJ, Labarca M, Oyanadel C, Del Valle Batalla F, Goles N, Chikina A, Lankar D, Segovia-Miranda F, Garcia C, Léger T, Gonzalez A, Espéli M, Lennon-Duménil AM, Yuseff MI, Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse, Cell Reports (2018), Dec;25(11):3110-3122.e6.

Obino D*, Diaz J*, Sáez JJ, Ibañez-Vega J, Sáez PJ, Alamo M, Lankar D, Yuseff MI, Vamp-7-dependent secretion at the immune synapse regulates antigen extraction and presentation in B-lymphocytes, Mol. Biol. Cell. (2017), Apr;28(7):890-897. * equal contribution

Bretou M, Kumari A, Malbec O, Moreau HD, Obino D, Pierobon P, Randrian V, Sáez PJ, Lennon-Duménil AM, Dynamics of the membrane-cytoskeleton interface in MHC class II-restricted antigen presentation, Immunol. Rev. (2016), Jul;272(1):39-51. All authors equally contributed to this work.

Ibáñez L, Abou-Ezzi G, Ciucci T, Amiot V, Belaïd N, Obino D, Mansour A, Rouleau M, Wakkach A, Blin-Wakkach C, Inflammatory Osteoclasts Prime TNFα-Producing CD4(+) T Cells and Express CX3 CR1, J. Bone Miner. Res. (2016), Oct;31(10):1899-1908.

Obino D, Farina F, Malbec O, Sáez PJ, Maurin M, Gaillard J, Dingli F, Loew D, Gautreau A, Yuseff MI, Blanchoin L, Théry M, Lennon-Duménil AM, Actin nucleation at the centrosome controls lymphocyte polarity, Nature Communications (2016), Mar;7:10969.

Vargas P, Maiuri P, Bretou M, Sáez PJ, Pierobon P, Maurin M, Chabaud M, Lankar D, Obino D, Terriac E, Raab M, Thiam HR, Brocker T, Kitchen-Goosen SM, Alberts AS, Sunareni P, Xia S, Li R, Voituriez R, Piel M, Lennon-Duménil AM, Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells, Nat. Cell Biol. (2016), Jan;18(1):43-53.

Reversat A^#, Yuseff MI^#, Lankar D*, Malbec O*, Obino D*, Maurin M, Penmatcha NV, Amoroso A, Sengmanivong L, Gundersen GG, Mellman I, Darchen F, Desnos C, Pierobon P, Lennon-Duménil AM, Polarity protein Par3 controls B-cell receptor dynamics and antigen extraction at the immune synapse, Mol. Biol. Cell. (2015), Apr;26(7):1273-85. ^#,*equal contributions

Obino D, Lennon-Duménil AM, A critical role for cell polarity in antigen extraction, processing, and presentation by B lymphocytes, Adv. Immunol. (2014), 123:51-67.

Ghislin S, Obino D, Middendorp S, Boggetto N, Alcaide-Loridan C, Deshayes F, LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro, BMC Cancer (2012), Oct;12:455.

Ghislin S, Obino D, Middendorp S, Boggetto N, Alcaide-Loridan C, Deshayes F, Junctional adhesion molecules are required for melanoma cell lines transendothelial migration in vitro, Pigment Cell Melanoma Res. (2011) Jun;24(3):504-11.