Dorian Obino

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EDUCATION

• PhD in Immuno-cell biology – Institut Curie, INSERM U932, Paris, France (2016)
• Magistère Européen de Génétique – Major: Immuno-genetics – Universités Paris VII & Paris V, Paris, France (2009-2012)
• Master in Genetics – Université Paris VII (Paris, France), (2010-2012)
• Bachelor (Licence) in Genetics – Université Paris VII, Paris, France (2009-2010)
• DUT (Diplôme Universitaire de Technologie) in Génie Biologique – Université du Sud Toulon Var (2007 – 2009)

RESEARCH EXPERIENCE

• Since October 2022: INSERM Research Scientist (Chargé de Recherche CRCN – CSS3) – Institut Pasteur, Paris, France, Pathogenesis of Vascular Infections unit, INSERM U1225, Head: Dr. Guillaume Duménil
• 2017-2022: Postdoctral Fellow – Institut Pasteur, Paris, France, Pathogenesis of Vascular Infections unit, INSERM U1225, Head: Dr. Guillaume Duménil
• 2012-2016: PhD Student – Institut Curie, Paris, France – INSERM U932, Head: Dr. Ana-Maria Lennon-Duménil
• Ap-Aug 2011: Trainee – St Jude Children’s Research Hospital, Memphis, TN, USA – Lab of Dr. Peter J. Murray
• June-Aug 2010: Trainee – Institut Jacques Monod, Paris, France – UMR 7592, Head: Pr. Catherine Alcaïde-Loridan, Supervisor: Dr. Frédérique Deshayes

FELLOWSHIP

• 2018-2020: Pasteur-Roux Postdoctoral Fellowship – Institut Pasteur Paris
• 2015-2016: 4^th year PhD Fellowship – Fondation pour la Recherche Médicale
• 2012-2015: PhD Fellowship – BioSPC Graduate School, Universités Paris V & VII

AWARDS

• 2016: Académie Nationale de Pharmacie, Prix de Thèse en Sciences Biologiques

COMMUNICATIONS

Invited speaker

• 2023: Institut de Recherche Saint Louis, Paris, France – “Innate immune response to meningococcal intravascular infection”
• 2018: Seoul National University, Seoul, Republic of Korea – “Development of a vessels-on-a-chip device to study meningococcal intravascular infection“
• 2016: Plenary session of the Académie Nationale de Pharmacie, Paris, France – “Molecular mechanisms regulating B lymphocyte polarization”

Oral presentations selected from abstract

• 2020: Departmental seminar Cell Biology & Infection, Online – “Dermal Lyve-1⁺ perivascular macrophages: Origin & functions in health & disease”
• 2016: Journées Scientifiques et Médicales, Institut Curie, Paris, France – “Elevator talk: My PhD in 180 seconds”
• 2016: 7^th Edition of the YRLS (Young Researchers in Life Science) Conference, Paris, France – “Actin nucleation at the centrosome controls lymphocyte polarity”
• 2013: SFI (French Society of Immunology) annual meeting, Paris, France – “Galectin-8 promotes efficient antigen processing and presentation by B lymphocytes”

Poster presentations

• 2019: Departmental retreat Cell Biology & Infection, San Feliu de Guíxols, Spain – “Dermal perivascular macrophages sample blood vessels in search of pathogens”
• 2015: Actin and microtubule cytoskeleton in cell motility and morphogenesis: an integrated view. J. Monod conference, Roscoff, France – “Actin nucleation at the centrosome controls lymphocyte polarity”
• 2013: SFI (French Society of Immunology) annual meeting, Paris, France – “Galectin-8 promotes efficient antigen processing and presentation by B lymphocytes”
• 2012: Current Trends in Biochemistry, Biophysics and Cell Biology, 39^th Winter School of Faculty of Biochemistry, Biophysics and Biotechnology, Zakopane, Poland – “Tuning B lymphocyte responses through cell polarity and lysosome exocytosis”

TEACHING   

• 2022: Scientific advisor for students – Double diplôme “Politique et identité du vivant” Sciences Po – Université Paris Cité – Licence Science du Vivant. Chair: F. Deshayes
• 2018, 2019 & 2020: Co-organizer & Teaching of the Practicum: “How to make a tube” – Master 2 Pasteur Course: Molecular Biology of the Cell, Chairs: C. Zurzolo & R. Bruzzone
• 2016 & 2017: Invited lecturer – Master2 “Biologie Cellulaire, Physiologie et Pathologie, Spécialité Biologie et Développement Cellulaires”, Course: Membrane dynamics and signaling, Université Paris Descartes, Chair: A. Benmerah
• 2015 & 2016: Teaching assistant – Bachelor’s degree, Practical course: Molecular biology, Université Paris VII,  Chair: V. Joliot
• 2013 & 2014: Invited lecturer – Bachelor’s degree, Course: Cell Biology, Magistère Européen de Génétique, Université Paris VII, Chair: C. Alcaïde-Loridan

PUBLICATIONS

Adé K*, Saenz Coronilla J*, Obino D, Weinberger T, Kaiser C, Mella S, Chen C, Katsimpardi L, Werts C, Li H, Dardenne P, Lallemand Y, Gomez Perdiguero E. Inflammation drives age-induced loss of tissue resident macrophages. BioRxiv (2022).10.02.510513.

Saez JJ, Diaz J, Ibanez J, Bozo JP, Cabrera Reyes F, Alamo M, Gobert FX, Obino D, Bono MR, Lennon-Dumenil AM, Yeaman C, Yuseff MI. The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells. Cell Biol. (2019). 218(7):2247-64.

Inoue D*, Obino D*, Pineau J, Farina F, Gaillard J, Guerin C, Blanchoin L, Lennon-Dumenil AM, Thery M. Actin filaments regulate microtubule growth at the centrosome. EMBO J. (2019). 38(11). *equal contribution

Obino D, Fetler L, Soza A, Malbec O, Saez JJ, Labarca M, Oyanadel C, Del Valle Batalla F, Goles N, Chikina A, Lankar D, Segovia-Miranda F, Garcia C, Léger T, Gonzalez A, Espéli M, Lennon-Duménil AM, Yuseff MI, Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse, Cell Reports (2018), Dec;25(11):3110-3122.e6.

Obino D*, Diaz J*, Sáez JJ, Ibañez-Vega J, Sáez PJ, Alamo M, Lankar D, Yuseff MI, Vamp-7-dependent secretion at the immune synapse regulates antigen extraction and presentation in B-lymphocytes, Mol. Biol. Cell. (2017), Apr;28(7):890-897. * equal contribution

Bretou M, Kumari A, Malbec O, Moreau HD, Obino D, Pierobon P, Randrian V, Sáez PJ, Lennon-Duménil AM, Dynamics of the membrane-cytoskeleton interface in MHC class II-restricted antigen presentation, Immunol. Rev. (2016), Jul;272(1):39-51. All authors equally contributed to this work.

Ibáñez L, Abou-Ezzi G, Ciucci T, Amiot V, Belaïd N, Obino D, Mansour A, Rouleau M, Wakkach A, Blin-Wakkach C, Inflammatory Osteoclasts Prime TNFα-Producing CD4(+) T Cells and Express CX3 CR1, J. Bone Miner. Res. (2016), Oct;31(10):1899-1908.

Obino D, Farina F, Malbec O, Sáez PJ, Maurin M, Gaillard J, Dingli F, Loew D, Gautreau A, Yuseff MI, Blanchoin L, Théry M, Lennon-Duménil AM, Actin nucleation at the centrosome controls lymphocyte polarity, Nature Communications (2016), Mar;7:10969.

Vargas P, Maiuri P, Bretou M, Sáez PJ, Pierobon P, Maurin M, Chabaud M, Lankar D, Obino D, Terriac E, Raab M, Thiam HR, Brocker T, Kitchen-Goosen SM, Alberts AS, Sunareni P, Xia S, Li R, Voituriez R, Piel M, Lennon-Duménil AM, Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells, Nat. Cell Biol. (2016), Jan;18(1):43-53.

Reversat A^#, Yuseff MI^#, Lankar D*, Malbec O*, Obino D*, Maurin M, Penmatcha NV, Amoroso A, Sengmanivong L, Gundersen GG, Mellman I, Darchen F, Desnos C, Pierobon P, Lennon-Duménil AM, Polarity protein Par3 controls B-cell receptor dynamics and antigen extraction at the immune synapse, Mol. Biol. Cell. (2015), Apr;26(7):1273-85. ^#,*equal contributions

Obino D, Lennon-Duménil AM, A critical role for cell polarity in antigen extraction, processing, and presentation by B lymphocytes, Adv. Immunol. (2014), 123:51-67.

Ghislin S, Obino D, Middendorp S, Boggetto N, Alcaide-Loridan C, Deshayes F, LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro, BMC Cancer (2012), Oct;12:455.

Ghislin S, Obino D, Middendorp S, Boggetto N, Alcaide-Loridan C, Deshayes F, Junctional adhesion molecules are required for melanoma cell lines transendothelial migration in vitro, Pigment Cell Melanoma Res. (2011) Jun;24(3):504-11.