Laboratoire

Dynamique structurale des macromolécules

Responsable

Marc Delarue

CNRS UMR3528

Département de Biologie structurale et chimie

• Adaptive immunity
• Biophysics
• DNA biosynthesis, modification, repair and degradation
• DNA repair
• Evolution
• Molecular and Structural Biology and Chemistry
• Molecular interactions
• Neurochemistry and neuropharmacology
• Replication
• Scientific computing
• Structural bioinformatics
• Structural biology

VOIR TOUSFermer

Présentation

Désolé, cet article est seulement disponible en Anglais Américain.

Membres

Marc Delarue

Responsable de Structure

Responsable

Pierre Raia

Etudiant(e) en thèse

Sandrine Rosario

Technicien(ne)

Zeineb Fourati-Kammoun

Post-doctorant(e)

Haidai Hu

Etudiant(e) en thèse

Thomas Ybert

Post-doctorant(e)

Frédéric Poitevin

Post-doctorant(e)

Ludovic Sauguet

Chercheur(euse) Permanent(e)

Jérôme Loc’h

Post-doctorant(e)

Projets

Development of methods in computational structural biology

We are developing new computational methods to calculate the electrostatics of proteins, understand their dynamical properties and simulate transitions between two known conformations of the same macromolecule.

DNA Replication and Synthetic Biology

We work with archaeal DNA polymerases to make them accept xeno-nucleotides and synthesize variants of DNA and RNA in vivo.

DNA Repair and Cancer

We study the mechanism of DNA Repair of (DNA) Double Strand Breaks  through the so-called Non-Homologous End Joining (NHEJ) process  in mammals using x-ray crystallography and structural studies of pol mu and Tdt

Ligand-gated Ion channels

 We study the structure and function of ligand-gated ion channels by X-ray crystallography to understand i) the gating mechanism (opening of the pore upon agonist binding) ii) the permeation mechanism (transport of ions through the pore) iii) […]

Logiciel

AquaSol

 We have developed a new way to calculate electrostatics properties of biological macromolecules in a polarizable solvent: AquaSol. We can then compute the solvent density around proteins as well as their SAXS spectra: AquaSAXS.

NOMAD-Ref

Please visit our older web page on Normal Mode Analysis and related subjects: Nomad-Ref

Financements

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Publications

• 2015
  Structural basis for a novel mechanism of DNA bridging and alignment in eukaryotic DSB DNA repair - Gouge J, Rosario S, Romain F, Poitevin F, Béguin P, Delarue M - The EMBO journal
• 2015
  Genuine open form of the pentameric ligand-gated ion channel GLIC - Fourati Z, Sauguet L, Delarue M - Acta crystallographica. Section D, Biological crystallography
• 2015
  Allosteric and hyperekplexic mutant phenotypes investigated on an α1 glycine receptor transmembrane structure - Moraga-Cid G, Sauguet L, Huon C, Malherbe L, Girard-Blanc C, Petres S, Murail S, Taly A, Baaden M, Delarue M, Corringer PJ - Proceedings of the National Academy of Sciences of the United States of America
• 2014
  Crystallographic studies of pharmacological sites in pentameric ligand-gated ion channels - Sauguet L, Shahsavar A, Delarue M - Biochimica et biophysica acta
• 2013
  Crystal structures of a pentameric ligand-gated ion channel provide a mechanism for activation - Sauguet L, Shahsavar A, Poitevin F, Huon C, Menny A, Nemecz À, Haouz A, Changeux JP, Corringer PJ, Delarue M - Proceedings of the National Academy of Sciences of the United States of America
• 2013
  Structures of intermediates along the catalytic cycle of terminal deoxynucleotidyltransferase: dynamical aspects of the two-metal ion mechanism - Gouge J, Rosario S, Romain F, Beguin P, Delarue M - Journal of molecular biology
• 2013
  Structural basis for ion permeation mechanism in pentameric ligand-gated ion channels - Sauguet L, Poitevin F, Murail S, Van Renterghem C, Moraga-Cid G, Malherbe L, Thompson AW, Koehl P, Corringer PJ, Baaden M, Delarue M - The EMBO journal
• 2013
  Structural basis for potentiation by alcohols and anaesthetics in a ligand-gated ion channel - Sauguet L, Howard RJ, Malherbe L, Lee US, Corringer PJ, Harris RA, Delarue M - Nature communications
• 2012
  Molecular recognition of canonical and deaminated bases by P. abyssi family B DNA polymerase - Gouge J, Ralec C, Henneke G, Delarue M - Journal of molecular biology
• 2012
  Structure and pharmacology of pentameric receptor channels: from bacteria to brain - Corringer PJ, Poitevin F, Prevost MS, Sauguet L, Delarue M, Changeux JP - Structure (London, England : 1993)
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  Voir la liste complète de publications

Contact

Marie-Claire Rozier

Phone: Ext. 39 45
Email: marie-claire.rozier@pasteur.fr
Unite de Dynamique Structurales des Macromolecules Biologiques
25-28 Rue du Docteur Roux
75015, Paris
France