Single cell gene expression and beyond • Research - Institut Pasteur

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Post-doc
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Deputy Director of Center
Deputy Director of Department
Deputy Director of National Reference Center
Deputy Head of Facility
Director of Center
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Honorary President of the Departement
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Behind Evolutionary Trees: Mathematics and Algorithms in Phylogenetics – Montevideo 2019

Scientific Fields

Diseases

Organisms

Applications

Technique

Starting Date

01

Jul 2019

Ending Date

05

Jul 2019

Time

09:00:00

28 Rue du Docteur Roux, Paris, France

Address

2019-07-01 09:00:00 2019-07-05 17:00:00 Europe/Paris Single cell gene expression and beyond Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […] 28 Rue du Docteur Roux, Paris, France Valentina Libri valentina.libri@pasteur.fr

Course Overview

The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions.

Program
This 5-days course covers the following topics:

Overview of technologies for single cells omics and fundamentals of NGS (0.5 day); Separation of single cells by FACS (0.5 day); Single cell RNA prep by droplet-based assays (10xGenomics) (0.5 day); Preparation and quantification of single cell RNA-Seq libraries (1 day); Introduction to microfluidics-based approaches for single cell profiling (0.5 day); Basics of single cell transcriptome data analysis using state-of-the-art data analysis pipelines (2 days)

Quality Controls, best practices and “tips and tricks” are discussed.

Audience
Scientists, students and Core Facility staff interested in applying advanced single-cell transcriptome sequencing in their research are welcome. Basic knowledge of molecular biology and basics in “R” are desirable.

Online registration: webcampus.pasteur.fr/ip/Single-cell2019

Co-directors:
Valentina Libri
Bernd Jagla
Milena Hasan

Deadline for application: 31 March 2020
Attendees: 12 participants
Contact: formation@pasteur.fr

Location

Location: 28 Rue du Docteur Roux, Paris, France

Published on: 12 Nov 2019

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