The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions.
This 5-days course covers the following topics:
Overview of technologies for single cells omics and fundamentals of NGS (0.5 day); Separation of single cells by FACS (0.5 day); Single cell RNA prep by droplet-based assays (10xGenomics) (0.5 day); Preparation and quantification of single cell RNA-Seq libraries (1 day); Introduction to microfluidics-based approaches for single cell profiling (0.5 day); Basics of single cell transcriptome data analysis using state-of-the-art data analysis pipelines (2 days)
Quality Controls, best practices and “tips and tricks” are discussed.
Scientists, students and Core Facility staff interested in applying advanced single-cell transcriptome sequencing in their research are welcome. Basic knowledge of molecular biology and basics in “R” are desirable.
Online registration: webcampus.pasteur.fr/ip/Single-cell2019
Deadline for application: 31 March 2020
Attendees: 12 participants