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  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Starting Date
03
Oct 1991
Ending Date
11
Oct 2017
Status
Ongoing
Members
8
Structures
1

About

NMR spectroscopy

800 MHz NMR spectrometer installed in Pasteur

Three NMR spectrometers with 11.7, 14 and 18 Tesla magnets (500 MHz, 600 MHz and 800Mhz), well suited for study of biomolecules are currently in use in the lab. The 500Mhz was upgraded in 2009. The 600Mhz has been upgraded in 2016, the 800Mhz has installed in 2016. Both spectrometers are equipped with four channels (1H, 13C, 15N, 2H).

The Varian NMR System 500 spectrometer is equipped with several probes (triple resonance, broadband, indirect detection).

The 600Mhz and 800Mhz spectrometers are equipped with the latest generation 5 mm triple resonance 1H{13C/15N} cryoprobe with increased sensitivity for both 1H and 13C.

The wet-lab, other facilities

We are currently using different tools for the expression, purification and biochemical characterisation of proteins.
The lab’s facilities include absorption and fluorescence spectrometers.
We have the practical knowledge and an access to numerous technical resources available at several Institut Pasteur’s technological platforms. Among them, the Biophysics Platform (microcalorimetry, circular dichroïsm, infrared spectroscopy, fluorescence, surface plasmon resonance…), Protein Microsequencing and Analysis, Production of Recombinant Proteins.

Fundings