We provide users with expertise in routine and advanced sample preparation and imaging techniques.
Depending on the question we embed samples by routine room temperature embedding and thin sectioning. We provide expertise in preservation and embedding by high-pressure freezing and freeze substitution. More advanced techniques include cryo-EM of vitreous sections. To localize proteins (or certain lipids) we use EM-immuno-labeling of thawed cryo-sections according to Tokuyasu.
Imaging includes routine scanning- and transmission EM, 3D EM by electron tomography or focused ion beam-SEM as well as cryo-EM of particles. We provide assistance in reconstructions and rendering for tomography and FIB-SEM. Together with the imagopole we provide support in developing work-flows for correlated light- and electron microscopy adapted to the questions asked. Finally, we provide help in quantitative analyses of the observations.
Our team will assist you in choosing the appropriate methods for sample preparation, imaging and data analyses.