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  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
20
Nov 2018
Status
Ongoing
Members
6
Structures
2

About

The Zebrafish Projects Hub (Zorgl’hub) aims to provide Pasteur research groups with the possibility to conduct short term zebrafish trial projects,

  • as a functional complement to existing projects using other models/cells or
  • to obtain proof of principle evidence to support grant applications.

Why the zebrafish (click here) ?

Zebrafish larva (72 hours, approx. size 3 mm) (left) and adult (3 months, approx. size 3 cm) (right).

Who ?

The two teams running the Zebrafish Project Hub have complementary expertise and are joining forces to cover several scientific fields of interest within the Pasteur campus, to offer you the knowledge and tools permitting the development of short-term zebrafish projects.

Unit Zebrafish Neurogenetics (ZEN):  developmental biology / cell biology / neuroscience
Resp. : Laure Bally-Cuif

Group Response to Infection in the Whole Organism (RIWO):  immunology / infection
Resp.: Jean-Pierre Levraud

What services?

  • Production of zebrafish embryos and adults
  • Zebrafish husbandry
  • Support to developmental biology, cell biology and neuroscience projects
  • Testing infection models

Prices cover only consumables and equipment depreciation.

The exact services offered and approximate prices can be downloaded here.

What we do not offer  : click here

Example of a pipeline:

a-d: Experimental pipeline: breeding (a), microinjections (b,c) and embryo analysis (d). e: her5:mCherry;cmcl2:gfp transgenic embryo used for the analysis of neural phenotypes (her5: midbrain-hindbrain boundary of the neural tube, cmcl2: cardiac myosin, used as a transgenic reporter). f,g: examples of larvae analysed for pathogen infections: larva infected with red bacteria and showing green neutrophils (f) and larvae showing red macrophages and green neutrophils (g).

Current projects

Identification of Tunneling Nanotubes in vivo Chiara Zurzolo, Anna Pepe, Diégo Cordero Cervantes, Department of Cell Biology & Infection, Pasteur Institute, Paris FR

Production of wildtype embryos to study microbiota-based resistance to bacterial infection in zebrafish larvae David Perez-Pascual, Bianca Audrain and Jean-Marc Ghigo, Genetics of Biofilms Unit, Institut Pasteur

Access Protocol

To place a request, please download the form here and email the completed form at zorglhub@pasteur.fr.

Choice of the projects is subject to decision by the Zorgl’hub committee.

The expected duration of each project is a maximum of 6 months, and a maximum of 2 projects can be run at a time by each team of the hub.

Criteria for selection include:

  • technical feasibility and scientific soundness for using the zebrafish model for the project
  • fitting with the scientific expertise of the Zorgl’hub teams (alternatively, more appropriate labs can be suggested)
  • project feasibility with available equipment on site (in the fish facilities and Zorgl’hub teams, or at Institut Pasteur)

The Zebrafish Projects Hub teams should be acknowledged in publications but no co-authorship is requested (unless the project develops into a collaboration).