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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Modular gene over-expression strategies for Candida albicans

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2012

Cabral V, Chauvel M, Firon A, Legrand M, Nesseir A, Bachellier-Bassi S, Chaudhari Y, Munro CA, d'Enfert C

Link to Pubmed [PMID] – 22328378

Methods Mol. Biol. 2012;845:227-44

Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.

http://www.ncbi.nlm.nih.gov/pubmed/22328378